EMAGE mouse gene expression spatial database

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EMAGE:15155

Bhlhe23 basic helix-loop-helix family, member e23 ( MGI:2153710)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010323. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010323_01	euxassay_010323_02	euxassay_010323_03	euxassay_010323_04

euxassay_010323_05	euxassay_010323_06	euxassay_010323_07	euxassay_010323_08	euxassay_010323_09

euxassay_010323_10	euxassay_010323_11	euxassay_010323_12	euxassay_010323_13	euxassay_010323_14

euxassay_010323_15	euxassay_010323_16	euxassay_010323_17	euxassay_010323_18	euxassay_010323_19

euxassay_010323_20	euxassay_010323_21	euxassay_010323_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15155_wholemount_strong.wlz

15155_wholemount_moderate.wlz

15155_wholemount_weak.wlz

15155_wholemount_possible.wlz

15155_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15155_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10 12 13 moderate expression: see section 06 07 08 14 15

midbrain mantle layer

strong

regional

strong expression: see section 09 12 13 moderate expression: see section 04 05 06 07 08 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37592
Entity Detected:	Bhlhe23, basic helix-loop-helix family, member e23 ( MGI:2153710)
Sequence:	sense strand is shown >T37592 CAAGATAGCCACACTTCTGCTGGCCAAAAACTACATCCTCATGCAGGCGCAGGCCCTGGAGGAGATGCGG CGCTTGGTAGCATACCTCAACCAAGGCCAGGGCCTCGCGGCGCCGGTGGCCGCCGCGCCTCTAACGCCCT TTGGCCAGGCAGCGATTTACCCGTTCTCGGCGGGCACTGCGCTCGGGCCCTGCCCAGACAAGTGTGCCAC CTTCTCAGGATCGCCCTCGGCGCTTTGCAAACACTGCGGCGAGAAGCCCTGAGGGCTCCGATCCCCTCTG CAGCCGCCTTTCTATTTAGACTGTTCCCTGTCCTCGCTGGCGGGACTCTATGAGAAAGCCCAGTACTCCG CTTACAGAACTGTTGGGTGCAGAATGAGAAGTCCGTCTGAGAAAGCCAGAGAGTTGGCGCTGGACTCTAG ACCCTGCCGCATCCACTGAACTATCTCAGCTAAAAGGCTACGGGAGTGAGCAGTTAGTGAATGGTCCCAC ATCTGACAGGTGACTGCCCAAGGCTCAGGAGCGCAGGCACAGAGCATCGCGCTTGACCACATCCTAGCGT TGGATGGGGAACCCAGCCCCTTCTAAGCCACTTGCAGGGCCTTGCTTGCTTCAACTAGACCTGTTCCTCA GGACGGGTGCGGCCCCTCACCTCGGGCCTCTGTTGGCTCCAAGACTCCAGACCCGGCCTAAAAGCAAAAT TACGCCGAGGTCCTTTGGAGAGGTCGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94574. Forward Primer - name:094574_F_cDNA_Bhlhb4, sequence:CAAGATAGCCACACTTCTGCTG; Reverse Primer - name:094574_N_SP6_cDNA_Bhlhb4, sequence:ACGACCTCTCCAAAGGACCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15151 same embryo
	EMAGE:15154 same embryo
	EMAGE:15153 same embryo
	EMAGE:15152 same embryo
	EurExpress:euxassay_010323 same experiment
	MGI:4823467 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source