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EMAGE:15194

Bbs1 Bardet-Biedl syndrome 1 (human) ( MGI:1277215)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194
"Pseudo-wholemount" of euxassay_010425. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010425_01 euxassay_010425_02 euxassay_010425_03 euxassay_010425_04
EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194
euxassay_010425_05 euxassay_010425_06 euxassay_010425_07 euxassay_010425_08 euxassay_010425_09
EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194
euxassay_010425_10 euxassay_010425_11 euxassay_010425_12 euxassay_010425_13 euxassay_010425_14
EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194
euxassay_010425_15 euxassay_010425_16 euxassay_010425_17 euxassay_010425_18 euxassay_010425_19
EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194 EMAGE:15194
euxassay_010425_20 euxassay_010425_21 euxassay_010425_22 euxassay_010425_23 euxassay_010425_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15194Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15194_wholemount_strong.wlz
15194_wholemount_moderate.wlz
15194_wholemount_weak.wlz
15194_wholemount_possible.wlz
15194_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15194_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37583
Entity Detected:Bbs1, Bardet-Biedl syndrome 1 (human) ( MGI:1277215)
Sequence:sense strand is shown

>T37583
GTTGATCGAGGGCTTAGATGTCGGCTGGTACTGATCCTCTGCTGTCCTTTGAAGTTGCAGGGGTGTGGGT
GCTGCCAGCTTGGTTTATAGGCACATGTGCTAAGCTTTCTCAACTGAAGAGGGGCTCCACTTTCTACCCT
GAAGCCCAGGAGTCATGGGGCTCTGTCCCCAGGTCCTAGACGAGCCACAGAGATTGCCAGAGGAGCTGGT
GCCAGAAGTAGACGGGGTCCGTGTGAGGAGCTAGAGCCACACAGCCAGCAGCTGCTCCCGACATGGGTGC
TCATGGCCAGAGGCACTGTTCTGAAATGGAATGCGGAGCTAGATAGCGGAGCTGGGGCTGGTGGCACTGG
GAGAGGTCTGGACCTGGGACCTGGGAAGTGACCCAAGAGGCAGATGCCGACAAGAGCCTGTCAGAGTGCA
CTTAGGTCCAGGAGCCCGAGATGGTCCTGAGGGTGGAATGTTCACTGTGCAGTGGCCAGAAGATGCCTGA
GCTCACGGAAAAGGTGCACACCAGGGGTGTGGCATAAAGCCGAAGACTGAAGGTAGATGTGCTTAGATCT
CAAGGGTCAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86296. Forward Primer - name:086296_F_cDNA_Bbs1, sequence:GTTGATCGAGGGCTTAGATGTC; Reverse Primer - name:086296_N_SP6_cDNA_Bbs1, sequence:CCTGACCCTTGAGATCTAAGCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15198 same embryo
 EMAGE:15195 same embryo
 EMAGE:15193 same embryo
 EMAGE:15196 same embryo
 EMAGE:15197 same embryo
 EurExpress:euxassay_010425 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS