EMAGE mouse gene expression spatial database

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EMAGE:15200

Zfp57 zinc finger protein 57 ( MGI:99204)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010422. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010422_01	euxassay_010422_02	euxassay_010422_03	euxassay_010422_04

euxassay_010422_05	euxassay_010422_06	euxassay_010422_07	euxassay_010422_08	euxassay_010422_09

euxassay_010422_10	euxassay_010422_11	euxassay_010422_12	euxassay_010422_13	euxassay_010422_14

euxassay_010422_15	euxassay_010422_16	euxassay_010422_17	euxassay_010422_18	euxassay_010422_19

euxassay_010422_20	euxassay_010422_21	euxassay_010422_22	euxassay_010422_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15200_wholemount_strong.wlz

15200_wholemount_moderate.wlz

15200_wholemount_weak.wlz

15200_wholemount_possible.wlz

15200_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15200_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 16 17

trigeminal v nerve

moderate

regional

moderate expression: see section 09 16

spinal cord

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

weak

regional

weak expression: see section 08

cervical ganglion

weak

regional

weak expression: see section 08

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 12 13 weak expression: see section 14 15

neural retina

strong

regional

strong expression: see section 02 03 04 05 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30557
Entity Detected:	Zfp57, zinc finger protein 57 ( MGI:99204)
Sequence:	sense strand is shown >T30557 TGGTACTGCGGAGAAGGCTCTACCACAGTGCAGGAAGCTAGTAACTGGGTGCTACGGAGGCACTTATTCC TTACTCTCTCCCTTTGCTGATATTATCTATTTTTCTTAAACCTTAGATTTCTAGAAGTGGGCACCAACAA ATTGTGGCTAGAAGCAGTCTGGAATAGAAGTCAAACGCCTAGGACCAGCCTGGCATTACCAAACAATGGC AGCTAGGAAACAGTCTTCCCAGCCATCCAGGACACCAGTCAGTTATGAGGACGTGGCAGTGTCTTTCACC CAGGAAGAATGGGAATATCTTACTTCTACACAGAAGACCCTTTACCAGAAAGTGATGTCAGAAACCTTCA AGAACCTGACATTTGTCGGAAGCAAGAAGAAACCTCAAGAACCTAGCTCAGATCTGCAAGATAAGAACGA GGAGCAGGAGAAGTCCTCCAGTTGCACAGGGGTATTCAAAGGTGGACCATTCTTTTTCTGTCTGACCTGT GGCAAATGTTTCAAAAAGAACACCTTCCTCTTTAATCACCAGTTTCCTGTGAGGTCCCGGAGGCTGGCAG TCACAAATCCACAAAGCCGCAAAGGCAAGGGCTACAAGGCTCAGCATCGTGGAGAGAGGCCTTTCTTTTG TAATTTCTGTGGCAAGACTTACCGTGATGCTTCTGGACTGAGCCGTCACCGACGTGCTCATTTAGGTTAT AGGCCCCGTTCATGCCCTGAGTGTGGAAAGTGTTTCCGGGATCAGTCTGAGGTCAACCGTCACCTGAAGG TACACCAAAACAAGCCAGCAGCTAGCAACCAGGCTGGCAACCAGGCTAGCAACCAGAGGCTGAAGAGTAG GGTTCCACCTACAACACCTAGATCCCAAGCGCCCGCCCTCAAGTATGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5686931), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59067. Forward Primer - name:059067_F_IRAV111_g09_Zfp57, sequence:TGGTACTGCGGAGAAGGC; Reverse Primer - name:059067_R_SP6_IRAV111_g09_Zfp57, sequence:CACATACTTGAGGGCGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15201 same embryo
	EMAGE:15199 same embryo
	EMAGE:15204 same embryo
	EMAGE:15202 same embryo
	EMAGE:15203 same embryo
	EurExpress:euxassay_010422 same experiment
	MGI:4829321 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source