EMAGE mouse gene expression spatial database

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EMAGE:15203

Slc32a1 solute carrier family 32 (GABA vesicular transporter), member 1 ( MGI:1194488)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010420. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010420_01	euxassay_010420_02	euxassay_010420_03	euxassay_010420_04

euxassay_010420_05	euxassay_010420_06	euxassay_010420_07	euxassay_010420_08	euxassay_010420_09

euxassay_010420_10	euxassay_010420_11	euxassay_010420_12	euxassay_010420_13	euxassay_010420_14

euxassay_010420_15	euxassay_010420_16	euxassay_010420_17	euxassay_010420_18	euxassay_010420_19

euxassay_010420_20	euxassay_010420_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15203_wholemount_strong.wlz

15203_wholemount_moderate.wlz

15203_wholemount_weak.wlz

15203_wholemount_possible.wlz

15203_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15203_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 06 08 09 10 11 12 13 14 15

telencephalon

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

hindbrain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30559
Entity Detected:	Slc32a1, solute carrier family 32 (GABA vesicular transporter), member 1 ( MGI:1194488)
Sequence:	sense strand is shown >T30559 GCCATTCAGGGCATGTTCGTGCTGGGCCTACCCTACGCCATCCTCCACGGCGGCTACCTGGGGTTGTTCC TCATCATCTTCGCCGCAGTGGTGTGCTGCTACACCGGCAAGATCCTCATCGCGTGCCTGTACGAGGAGAA CGAAGACGGGGAGGTGGTGCGCGTGCGGGACTCGTATGTGGCCATAGCTAACGCATGCTGCGCTCCTCGA TTCCCCACCCTGGGCGGCCGCGTGGTCAATGTGGCGCAGATCATCGAGCTGGTGATGACGTGTATCTTGT ACGTCGTGGTGAGCGGCAACCTCATGTACAACAGTTTCCCGGGGCTGCCCGTGTCGCAGAAGTCCTGGTC CATCATAGCCACAGCGGTGCTGCTGCCCTGCGCCTTCCTGAAGAATCTCAAGGCCGTGTCCAAGTTCAGT CTGCTGTGTACGCTGGCCCACTTCGTCATCAACATCCTGGTCATCGCTTACTGTCTCTCTCGCGCGCGTG ATTGGGCCTGGGAGAAGGTGAAGTTCTACATCGACGTCAAGAAGTTTCCCATCTCCATTGGCATCATCGT GTTCAGCTACACGTCGCAGATCTTCCTGCCCTCTCTCGAAGGCAACATGCAGCAGCCCAGCGAATTCCAC TGCATGATGAACTGGACACACATCGCCGCCTGCGTGCTCAAGGGTCTCTTCGCGCTCGTCGCCTACCTCA CCTGGGCCGACGAGACCAAGGAAGTCATCACGGATAACCTGCCCGGCTCCATCCGCGCCGTGGTCAACCT CTTCCTGGTGGCCAAGGCGCTGCTGTCCTATCCGTTGCCCTTCTTCGCGGCCGTCGAAGTGCTGGAGAAG TCTCTCTTCCAGGAAGGCAGTCGCGCCTTCTTCCCCGCCTGCTATGGAGGCGACGGTCGCCTTAAGTCCT GGGGGCTGACGCTGCGCTGCGCGCTGGTGGTCTTCACGCTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5686843), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59058. Forward Primer - name:059058_F_IRAV111_h05_Viaat, sequence:GCCATTCAGGGCATGTTC; Reverse Primer - name:059058_R_SP6_IRAV111_h05_Viaat, sequence:AGCAGCGTGAAGACCACC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15201 same embryo
	EMAGE:15199 same embryo
	EMAGE:15204 same embryo
	EMAGE:15202 same embryo
	EMAGE:15200 same embryo
	EurExpress:euxassay_010420 same experiment
	MGI:4828194 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source