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EMAGE:15313

Trim9 tripartite motif-containing 9 ( MGI:2137354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313
"Pseudo-wholemount" of euxassay_010509. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010509_01 euxassay_010509_02 euxassay_010509_03 euxassay_010509_04
EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313
euxassay_010509_05 euxassay_010509_06 euxassay_010509_07 euxassay_010509_08 euxassay_010509_09
EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313
euxassay_010509_10 euxassay_010509_11 euxassay_010509_12 euxassay_010509_13 euxassay_010509_14
EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313
euxassay_010509_15 euxassay_010509_16 euxassay_010509_17 euxassay_010509_18 euxassay_010509_19
EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313 EMAGE:15313
euxassay_010509_20 euxassay_010509_21 euxassay_010509_22 euxassay_010509_23 euxassay_010509_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15313Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15313_wholemount_strong.wlz
15313_wholemount_moderate.wlz
15313_wholemount_weak.wlz
15313_wholemount_possible.wlz
15313_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15313_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
thalamus mantle layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 15 16 18 moderate expression: see section 14 17
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23 moderate expression: see section 02 13 15 16 17
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 09 10 11 12 13 14
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
pons mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
pons ventricular layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15
spinal cord ventricular layer
strong strong
regionalstrong expression: see section 10 11
lower jaw molar
moderate moderate
regionalmoderate expression: see section 08 19
upper jaw molar
moderate moderate
regionalmoderate expression: see section 08 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30552
Entity Detected:Trim9, tripartite motif-containing 9 ( MGI:2137354)
Sequence:sense strand is shown

>T30552
ACCAACAGAGCAAAGCCGCGGCCCTCAAGTGCCAGCTCTGCGAGAAGGCGCCCAAGGAAGCCACGGTCAT
GTGTGAACAGTGCGATGTCTTCTACTGCGACCCTTGCCGTCTGCGCTGCCACCCGCCCCGGGGTCCCCTA
GCCAAACACCGTCTGGTGCCCCCGGCCCAGGGCCGCGTCAGCCGGCGGCTGAGCCCGCGCAAGGTCTCCA
CCTGCACAGACCACGAGCTGGAGAACCACAGCATGTACTGCGTGCAATGCAAGATGCCCGTTTGCTACCA
GTGCCTAGAGGAGGGCAAACACTCCAGCCACGAAGTCAAGGCTTTGGGGGCTATGTGGAAATTGCACAAG
AGCCAGCTCTCCCAGGCCCTGAATGGATTGTCGGACAGGGCCAAAGAAGCCAAGGAGTTCCTGGTGCAGC
TCCGCACCATGGTACAACAGATCCAGGAAAACAGTGTGGAGTTTGAGGCCTGCCTGGTGGCCCAGTGTGA
TGCGCTCATCGATGCCCTAAACCGAAGGAAGGCTCAGCTGCTGGCCCGGGTCAACAAGGAGCATGAACAC
AAGCTGAAGGTGGTTCGGGACCAGATCTCTCACTGCACGGTGAAGCTGCGTCAGACCACGGGCCTCATGG
AGTACTGCTTGGAAGTGATTAAGGAGAACGACCCCAGTGGCTTTTTGCAGATTTCAGATGCTCTCATAAG
GCGAGTTCACTTAACTGAGGACCAGTGGGGGAAGGGCACCCTCACTCCCAGGATGACCACAGACTTTGAT
CTGAGCCTGGACAACAGCCCTCTGCTGCAGTCTATTCACCAACTGGACTTCGTGCAAGTGAAAGCCTCCT
CTCCAGTCCCAGCAACCCCCATCCTACAGCTGGAGGAGTGTTGCACCCACAACAACAGTGCTACGCTGTC
CTGGAAACAGCCCCCTCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5684877), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59075. Forward Primer - name:059075_F_IRAV111_f04_Trim9, sequence:ACCAACAGAGCAAAGCCG; Reverse Primer - name:059075_R_SP6_IRAV111_f04_Trim9, sequence:ACAGAGGGGGCTGTTTCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15316 same embryo
 EMAGE:15314 same embryo
 EMAGE:15312 same embryo
 EMAGE:15315 same embryo
 EMAGE:15311 same embryo
 EurExpress:euxassay_010509 same experiment
 MGI:4828921 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS