EMAGE mouse gene expression spatial database

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EMAGE:15316

Enox1 ecto-NOX disulfide-thiol exchanger 1 ( MGI:2444896)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010501. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010501_01	euxassay_010501_02	euxassay_010501_03	euxassay_010501_04

euxassay_010501_05	euxassay_010501_06	euxassay_010501_07	euxassay_010501_08	euxassay_010501_09

euxassay_010501_10	euxassay_010501_11	euxassay_010501_12	euxassay_010501_13	euxassay_010501_14

euxassay_010501_15	euxassay_010501_16	euxassay_010501_17	euxassay_010501_18	euxassay_010501_19

euxassay_010501_20	euxassay_010501_21	euxassay_010501_22	euxassay_010501_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15316_wholemount_strong.wlz

15316_wholemount_moderate.wlz

15316_wholemount_weak.wlz

15316_wholemount_possible.wlz

15316_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15316_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 10 11 15 17 18 19 20 21 22 23 weak expression: see section 01 09 12 13 14 16

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 18 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 16 17 18

spinal cord

moderate

regional

moderate expression: see section 07 08 10 11 weak expression: see section 09 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 15 16

neural retina

moderate

regional

moderate expression: see section 02 03 04 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30547
Entity Detected:	Enox1, ecto-NOX disulfide-thiol exchanger 1 ( MGI:2444896)
Sequence:	sense strand is shown >T30547 GGGTCGAGCACGGATAAAAAGGATTCCGGCCGCCTGCACGTGGATTTTGCTCAGGCCAGGGATGACTTCT ATGAGTGGGAATGCAAGCAAAGGATGCGCGCCCGGGAGGAGCGGCATCGACGCAAACTGGAAGAGGACAG GCTGCGGCCTCCCTCGCCGCCGGCCATCATGCACTACTCGGAGCACGAAGCTGCCCTGCTGGCTGACAAA CTGAAAGATGACAGCAAGTTCTCTGAGGCTATCACAGTTCTGCTGTCCTGGATCGAGCGTGGGGAGGTGA ACCGGCGCTCGGCAAACCAGTTCTACTCCATGGTTCAGTCGGCCAACAGCCACGTGCGCAGGCTCATGAA TGAAAAGGCTACCCACGAGCAGGAAATGGAGGAAGCAAAGGAGAACTTCAAAAATGCCTTAACGGGGATC CTCACTCAATTTGAGCAGATTGTGGCCGTTTTCAACGCTTCTACCAGACAAAAAGCTTGGGACCATTTCT CGAAAGCCCAGCGCAAGAACATAGACATTTGGCGAAAGCACTCTGAGGAGCTCCGGAACGCTCAAAGCGA GCAGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5703279), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59110. Forward Primer - name:059110_F_IRAV111_e01_D230005D02Rik, sequence:GGGTCGAGCACGGATAAA; Reverse Primer - name:059110_R_SP6_IRAV111_e01_D230005D02Rik, sequence:GAGCTGCTCGCTTTGAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15314 same embryo
	EMAGE:15312 same embryo
	EMAGE:15315 same embryo
	EMAGE:15311 same embryo
	EMAGE:15313 same embryo
	EurExpress:euxassay_010501 same experiment
	MGI:4824556 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source