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EMAGE:15369

Sf3a3 splicing factor 3a, subunit 3 ( MGI:1922312)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369
"Pseudo-wholemount" of euxassay_010567. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010567_01 euxassay_010567_02 euxassay_010567_03 euxassay_010567_04
EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369
euxassay_010567_05 euxassay_010567_06 euxassay_010567_07 euxassay_010567_08 euxassay_010567_09
EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369
euxassay_010567_10 euxassay_010567_11 euxassay_010567_12 euxassay_010567_13 euxassay_010567_14
EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369 EMAGE:15369
euxassay_010567_15 euxassay_010567_16 euxassay_010567_17 euxassay_010567_18 euxassay_010567_19
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euxassay_010567_20 euxassay_010567_21 euxassay_010567_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15369Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15369_wholemount_strong.wlz
15369_wholemount_moderate.wlz
15369_wholemount_weak.wlz
15369_wholemount_possible.wlz
15369_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15369_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30823
Entity Detected:Sf3a3, splicing factor 3a, subunit 3 ( MGI:1922312)
Sequence:sense strand is shown

>T30823
CGGAAGCACCCAAATGAGATCTGTGTGCCAATGTCAGTGGAATTTGAGGAGCTGCTGAAGGCTCGAGAGA
ATCCAAGTGAAGAGGCACAAAACTTGGTGGAGTTCACAGATGAAGAGGGATATGGTCGCTACCTTGATCT
CCATGACTGCTACCTCAAATACATTAACCTGAAGGCATCTGAGAAGCTGGATTACATCACATACCTGTCC
ATCTTTGACCAGTTGTTTGACATTCCTAAAGAAAGAAAGAATGCAGAGTACAAAAGATACCTGGAGATGC
TGCTGGAGTACCTACAGGATTACACAGATAGAGTGAAGCCCCTTCAGGATCAGAACGAGCTCTTTGGGAA
GATTCAGACCGACTTTGAGAAGAAGTGGGATAATGGCACCTTTCCTGGATGGCCGAAAGAGACAAGCAGC
GCCCTGACTCACGCGGGAGCCCATCTTGATCTCTCCGCATTCTCCTCCTGGGAGGAGTTGGCATCCCTGG
GTCTGGATAGGTTGAAATCTGCACTATTGGCATTGGGCCTGAAGTGTGGCGGGACCCTAGAAGAACGAGC
TCAGAGGCTCTTCAGCACCAAAGGAAAGTCTTTGGAGTCACTCGACACTTCTCTGTTTGCCAAAAATCCC
AAGTCAAAGGGCACAAAAAGAGACACTGAGAGGAACAAAGACATTGCTTTCCTGGAAGCCCAGATCTATG
AATACGTGGAGATTCTCGGGGAACAGCGACAGCTCACTCATGAAAATGTTCAGCGCAAGCAAGCCAGGAC
AGGCGAGGAGCGGGAGGAGGAGGAGGAGGAGCAGATCAGCGAGAGCGAAAGTGAGGACGAAGAGAATGAG
ATCATTTACAACCCCAAGAACCTGCCGCTGGGCTGGGACGGCAAGCCCATCCCCTAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3598647), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15665. Forward Primer - name:015665_F_IRAV16-19_P10_Sf3a3, sequence:CGGAAGCACCCAAATGAG; Reverse Primer - name:015665_R_SP6_IRAV16-19_P10_Sf3a3, sequence:AGTAGGGGATGGGCTTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15368 same embryo
 EMAGE:15366 same embryo
 EMAGE:15367 same embryo
 EMAGE:15370 same embryo
 EMAGE:15365 same embryo
 EurExpress:euxassay_010567 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS