EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15379

Naga N-acetyl galactosaminidase, alpha ( MGI:1261422)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010563. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010563_01	euxassay_010563_02	euxassay_010563_03	euxassay_010563_04

euxassay_010563_05	euxassay_010563_06	euxassay_010563_07	euxassay_010563_08	euxassay_010563_09

euxassay_010563_10	euxassay_010563_11	euxassay_010563_12	euxassay_010563_13	euxassay_010563_14

euxassay_010563_15	euxassay_010563_16	euxassay_010563_17	euxassay_010563_18	euxassay_010563_19

euxassay_010563_20	euxassay_010563_21	euxassay_010563_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15379_wholemount_strong.wlz

15379_wholemount_moderate.wlz

15379_wholemount_weak.wlz

15379_wholemount_possible.wlz

15379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 15 16 17 18 19 20

humerus

weak

regional

weak expression: see section 01 02 20 21

femur

weak

regional

weak expression: see section 04 19 20

diencephalon roof plate

weak

regional

weak expression: see section 13

choroid invagination

weak

regional

weak expression: see section 06 07 08 09 10 11 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 04 weak expression: see section 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

mandible

moderate

regional

moderate expression: see section 04 05 06 07 08 09 15 16 17 18 19 weak expression: see section 03 10 14 20

maxilla

moderate

regional

moderate expression: see section 04 05 06 07 08 09 15 16 17 18 19 weak expression: see section 10 14 20

orbito-sphenoid

moderate

regional

moderate expression: see section 04 21 22 weak expression: see section 01 02 03 05

clavicle

moderate

regional

moderate expression: see section 06 15 weak expression: see section 03 04 05 07 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30836
Entity Detected:	Naga, N-acetyl galactosaminidase, alpha ( MGI:1261422)
Sequence:	sense strand is shown >T30836 CCTGGGCACTGGAATGACCCAGACATGCTGCTCATCGGGAACTTTGGCCTGAGCTTCGATGAATCCCGGG CACAAATGGCCCTGTGGACAGTGCTCGCAGCCCCCCTCCTCATGTCCACGGACCTGCGTACCATCTCCCC TCAGAATATGGACATTCTTCAGAACCCACTCATGATCAAAATCAATCAGGACCCCTTAGGCATCCAGGGA CGCAGGATCTTAAAGAGCAAATCCCATATCGAAGTGTTCAAGAGGTATCTGTCTAACCAGGCCAGTGCCC TGGTTTTCTTCAGCCGCAGGACAGACATGCCTTTCCGCTTTCATTGCTCCCTCTTGGAACTAAACTACCC CAAAGGCAGAGTGTATGAGGGCCAGAACGTCTTCACAGGAGATATCTTCAGTGGTCTCCAGACTGAAGTC AACTTCACTGTGATCATCAATCCTTCGGGGGTTGTGATGTGGTACCTCTATCCCATCAAAGACCTGGGGA TATCCACGATGATGTCCCACTGGTGAGGACCCAAGAATGACAGGCTACGGCAGCCCCACTGAGCCTGGAC CACAGAGCCTTGACAGGCCGAGGACATGGCAGGGCTCTGTCCTCCACAGGCTGCTTGGTGATGTGACCTC CACCCCCCAAAGTGCATTTGCAGGGCCCAGGTTCAATGCCAGGTCCAGCTGAAGCAGGGGCCTTCTTGGA AGCTTGGAACTCTTGGGACAGTTTCCTGTGGCCTTCCGGCCTCTGCCCCTACACAGCTCTGCAGATTTCA CTCGGCCAGCCTCCCGAGCCCCCGCAGGCAGGGTCCTGATGCCCACTGGACTCCAGCCTCTGACTTTGCT GACTCAGAAATCAGGATTTGGAATTTTTAAAATTAAGAAGAGAGACCTGACTCCTTGCCAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4019197), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16800. Forward Primer - name:016800_F_IRAV20-23_J03_Naga, sequence:CCTGGGCACTGGAATGAC; Reverse Primer - name:016800_R_SP6_IRAV20-23_J03_Naga, sequence:TCCTGGCAAGGAGTCAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15378 same embryo
	EMAGE:15377 same embryo
	EMAGE:15381 same embryo
	EMAGE:15380 same embryo
	EMAGE:15376 same embryo
	EurExpress:euxassay_010563 same experiment
	MGI:4826587 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source