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EMAGE:15409

Tank TRAF family member-associated Nf-kappa B activator ( MGI:107676)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409
"Pseudo-wholemount" of euxassay_010622. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010622_01 euxassay_010622_02 euxassay_010622_03 euxassay_010622_04
EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409
euxassay_010622_05 euxassay_010622_06 euxassay_010622_07 euxassay_010622_08 euxassay_010622_09
EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409
euxassay_010622_10 euxassay_010622_11 euxassay_010622_12 euxassay_010622_13 euxassay_010622_14
EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409 EMAGE:15409
euxassay_010622_15 euxassay_010622_16 euxassay_010622_17 euxassay_010622_18 euxassay_010622_19
EMAGE:15409
euxassay_010622_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15409Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15409_wholemount_strong.wlz
15409_wholemount_moderate.wlz
15409_wholemount_weak.wlz
15409_wholemount_possible.wlz
15409_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15409_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 08 14 15
mandible
moderate moderate
single cellmoderate expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19
maxilla
moderate moderate
single cellmoderate expression: see section 05 06 07 10 11 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31153
Entity Detected:Tank, TRAF family member-associated Nf-kappa B activator ( MGI:107676)
Sequence:sense strand is shown

>T31153
AAGAATCCGCCAAGGGTCTCAACATCCCTCTGCATCACGAAAGGGATAATATAGAGAAGACTTTCTGGGA
CCTTAAAGAAGAATTTCATAGGATTTGCTTGCTAGCAAAAGCACAGAAAGACCACTTAAGCAAACTTAAT
ATACCAGATATTGCAACTGACACACAGTGTTCTGTGCCTATACAGTGTACTGATAAAACAGAGAAACAAG
AAGCGCTGTTTAAGCCCCAGGCTAAAGATGATATAAATAGAGGTATGTCGTGCGTCACAGCTGTCACACC
AAGAGGACTGGGCCGGGATGAGGAAGATACCTCTTTTGAATCACTTTCTAAATTCAATGTCAAGTTTCCG
CCTATGGACAATGACTCTATTTTTCTACATAGCACTCCAGAGGCCCCAAGCATCCTTGCTCCTGCCACAC
CTGAGACAGTGTGCCAGGACCGATTTAATATGGAAGTCAGAGACAACCCAGGAAACTTTGTTAAAACAGA
AGAAACTTTATTTGAAATTCAGGGAATTGACCCCATAACTTCAGCTATACAAAACCTTAAAACAACTGAC
AAAACAAACCCCTCAAATCTTAGAGCGCCGTGTTTGCCAGCTGGAGACCACAATGTGTTCTATGTAAATA
CGTTCCCACTTCAAGACCCGCCTGACGCACCTTTTCCCTCACTGGATTCCCCAGGAAAGGCTGTCCGAGG
ACCACAGCAGCCCTTTTGGAAGCCTTTTCTTAACCAAGACACTGACTTAGTGGTACCAAGTGATTCAGAC
TCAGAGCTCCTTAAACCTCTAGTGTGTGAATTCTGTCAAGAGCTTTTCCCACCATCCATTACATCCAGAG
GGGATTTCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3492937), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 53373. Forward Primer - name:053373_F_IRAV44_g09_Tank, sequence:AAGAATCCGCCAAGGGTC; Reverse Primer - name:053373_R_SP6_IRAV44_g09_Tank, sequence:GGAGGAAATCCCCTCTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15410 same embryo
 EMAGE:15411 same embryo
 EMAGE:15406 same embryo
 EMAGE:15407 same embryo
 EMAGE:15408 same embryo
 EurExpress:euxassay_010622 same experiment
 MGI:4828596 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS