Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15411

Eif4ebp2 eukaryotic translation initiation factor 4E binding protein 2 ( MGI:109198)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411
"Pseudo-wholemount" of euxassay_010591. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010591_01 euxassay_010591_02 euxassay_010591_03 euxassay_010591_04
EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411
euxassay_010591_05 euxassay_010591_06 euxassay_010591_07 euxassay_010591_08 euxassay_010591_09
EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411
euxassay_010591_10 euxassay_010591_11 euxassay_010591_12 euxassay_010591_13 euxassay_010591_14
EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411 EMAGE:15411
euxassay_010591_15 euxassay_010591_16 euxassay_010591_17 euxassay_010591_18 euxassay_010591_19
EMAGE:15411 EMAGE:15411 EMAGE:15411
euxassay_010591_20 euxassay_010591_21 euxassay_010591_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15411Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15411_wholemount_strong.wlz
15411_wholemount_moderate.wlz
15411_wholemount_weak.wlz
15411_wholemount_possible.wlz
15411_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15411_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30862
Entity Detected:Eif4ebp2, eukaryotic translation initiation factor 4E binding protein 2 ( MGI:109198)
Sequence:sense strand is shown

>T30862
CTGCCCTCTGCCCAGTTAAGGTTAGGTTGATGAATAAGCCCTTGGATTATTCTGTGGAGCTGACCAGTCC
ACAGAAGAAAGCAGTCCCTGTAGCTTCCCTGGTCATTTCCCAAGAATCTTCCTGCCCTGTTGAGACTTGC
CCCAAGTCTAGGGCAGTCTCCAAGCAGCACCTCTGCTTGTAGGGGTTGGGGGTGAAGGAGGTTTAGTGCC
CATTGTGTTCCTGGGCTCTCCCTGTCCTTCCCTACAGCCCACTACTGGTGGAGCGCTGGATGTGGGGGAC
ATTTGTCCTCAGCTCTGGAGCACAGTCCTGTACCTCCTGCACCTCTGCTGCATTCCTGGGACATGTGACA
GCAGCCTCCCCTTTCTCTGGGAGGAAGGCTTCTGTCTTGTCTTGGGTGTGATAGCTCATCATCCCCCCCC
CCCCCATTCCTTTACCCATTTCATTGGCACGGGTATTTTTTAAGCCCTCCTGAAGGGACCCCTTGGTGAC
CAGCTGGTCTTTGCCTGTCTGACATTCTTTCTACCTGCATCCCGTAGGCAGAGTCTGCCCTGGCACACCC
GTGGCTCTGTCTGCTTTTAGAGCAGAAGTGCCAACACCTTGGCCTGCACCTGGTGAGTCCAGCCTGCTCT
CAGCGGTCTGCCTGAGAATACATCAGGGGCCTCTGAAGAGGACTGAGCCGCCGTGCAGCCAGCCTCGGGG
GTCATGGTTTTAAAACCAGGTGTACCTTACGTTGGGTGTCCTGCAGGTTACTATACCTTGAGTTTAAAGT
CGACTTCCTCTTACATTTCTCCCCTGCTTTGGATCTGCTTTGTGCTTGGCTGCTGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4218820), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17135. Forward Primer - name:017135_F_IRAV24-27_C05_Eif4ebp2, sequence:CTGCCCTCTGCCCAGTTA; Reverse Primer - name:017135_R_SP6_IRAV24-27_C05_Eif4ebp2, sequence:CTCAGCAGCCAAGCACAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15410 same embryo
 EMAGE:15406 same embryo
 EMAGE:15407 same embryo
 EMAGE:15408 same embryo
 EMAGE:15409 same embryo
 EurExpress:euxassay_010591 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS