EMAGE mouse gene expression spatial database

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EMAGE:15589

Rhbdl2 rhomboid, veinlet-like 2 (Drosophila) ( MGI:3608413)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010755. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010755_01	euxassay_010755_02	euxassay_010755_03	euxassay_010755_04

euxassay_010755_05	euxassay_010755_06	euxassay_010755_07	euxassay_010755_08	euxassay_010755_09

euxassay_010755_10	euxassay_010755_11	euxassay_010755_12	euxassay_010755_13	euxassay_010755_14

euxassay_010755_15	euxassay_010755_16	euxassay_010755_17	euxassay_010755_18	euxassay_010755_19

euxassay_010755_20	euxassay_010755_21	euxassay_010755_22	euxassay_010755_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15589_wholemount_strong.wlz

15589_wholemount_moderate.wlz

15589_wholemount_weak.wlz

15589_wholemount_possible.wlz

15589_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15589_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 14 15 16 17 18 19 20

fibula

moderate

regional

moderate expression: see section 01 02

tibia

moderate

regional

moderate expression: see section 01 02

femur

moderate

regional

moderate expression: see section 03 04 weak expression: see section 20 21 22

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 22 weak expression: see section 02

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 16 17 18 19 20 21 22

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 06 07 23

clavicle

moderate

regional

moderate expression: see section 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38150
Entity Detected:	Rhbdl2, rhomboid, veinlet-like 2 (Drosophila) ( MGI:3608413)
Sequence:	sense strand is shown >T38150 AAGGTAAAGACTTCCCCAGGAGCAGAAAGGTCCACAGGATCGTCTCCAAGTGGATGCTTCCTGAGCCAGT CCGAAGAACATACTTAGAGAGAGCCAACTGCCTCCCGCCGCCCCTGTTCATCATCCTCATCAGTCTGGCT GAGGTAAACGCCGCCCTGCTGAGGGGCCTCACATCACTTCAGCCTGGAGGCTGCGAAGTCAGGACAAAAC CTACCTAGAGAAGAAACCATGTAGGAAGAGAGCACAGAGGGACGGGAGTTGCGCGTATGAAGCCTCCACC CCCTACCAGCTCCTGAGCTGATTTGCCCAGTCACTGCCCACCCAGGGGTTGGTTTCATCCACATCAAAAA TCCGCTGCCAGGGTTGACTTCGGGAGCTTCATTTCGTTTTAGCTAGAGCGATCTTTAGAAACCTTGGGCC TGCTAATTAGCCTTTTTATTTTTATTTTATTTTATTCTTTTGAGATGGGATTTGACATTATCACCCAGGC TGACCTCTCACTCACTATGTAGCTCAGGCTGGCCACACACTCACTATAATCCTTTCGTCATAATCACCTG GGTGTTGCGATTCTAGGCATGCACCACCATGCTCAGCTTGCAAATTGACTTTATCTCATGAAACAAAATA GACTGGTTGGTCAGGTCAGTCTGGGGTATATATTTAGAACAGTCACCAGCTGCCTGAGCTTTCGGAGGGA AAATGTGGCATTTGCTGTGGGTGTCTACTTTGGGTGGACGGGATATATTCTCAGACTATGACCTGCTTAG CTTTCTTGGTTTCCCGAAGGAGGGCAAAGCTCAGAGAGGTGAGGTGAGTCACCCAAGGGTGCGTCTCTGT GACTGGGAAGGGCCAGGTTCCTCCCGTGCTTTGTGATAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105722. Forward Primer - name:105722_F_cDNA_9130416B15, sequence:AAGGTAAAGACTTCCCCAGGAG; Reverse Primer - name:105722_N_SP6_cDNA_9130416B15, sequence:GTATCACAAAGCACGGGAGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15590 same embryo
	EMAGE:15587 same embryo
	EMAGE:15586 same embryo
	EMAGE:15585 same embryo
	EMAGE:15588 same embryo
	EurExpress:euxassay_010755 same experiment
	MGI:4827727 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source