EMAGE mouse gene expression spatial database

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EMAGE:15815

2310045N14Rik RIKEN cDNA 2310045N14 gene ( MGI:1924005)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013221. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013221_01	euxassay_013221_02	euxassay_013221_03	euxassay_013221_04

euxassay_013221_05	euxassay_013221_06	euxassay_013221_07	euxassay_013221_08	euxassay_013221_09

euxassay_013221_10	euxassay_013221_11	euxassay_013221_12	euxassay_013221_13	euxassay_013221_14

euxassay_013221_15	euxassay_013221_16	euxassay_013221_17	euxassay_013221_18	euxassay_013221_19

euxassay_013221_20	euxassay_013221_21	euxassay_013221_22	euxassay_013221_23	euxassay_013221_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15815_wholemount_strong.wlz

15815_wholemount_moderate.wlz

15815_wholemount_weak.wlz

15815_wholemount_possible.wlz

15815_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15815_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 05 20 23 24 weak expression: see section 04

upper leg muscle

moderate

regional

moderate expression: see section 01 02 03 05 06 17 18 19 20 21 22 weak expression: see section 04

diaphragm

moderate

regional

moderate expression: see section 01 02 03 05 06 07 08 09 17 18 19 20 21 22 23 weak expression: see section 04 16

shoulder rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03 23 24

foot mesenchyme

moderate

regional

moderate expression: see section 07 08 17 18 19

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03 21 23 24 weak expression: see section 04

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue muscle

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

tail mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39572
Entity Detected:	2310045N14Rik, RIKEN cDNA 2310045N14 gene ( MGI:1924005)
Sequence:	sense strand is shown >T39572 CCAGAATGACCAATTCTCTTCCGAGAAAGAAAAGATTACCAAACTTAACAAGAAAAATTCAGAGAAGACC AGTGCATTAGAAGTGCAGAGGAGTTGTGTCATATGTCACGTGGAAAGACAAGACAAGATAGAATTTGTTC AGAGGAATTCCACAGAGCTAAGAGAGAATGCAGGTGCAAAAGATGCTCCATGCATAGAAAGTCTGGAGAA ACAAGAAAGCAACAACCTGAGAGATAAACAAGACACTGAAGATAACAATGGAGGAGAAAATGGGATTTCT TCTAAGGACGATTCCAGAGGAGTAGAAGCTGAGAACAGAGCTTCAGCAGCTGCAAGTGGAGCAGTGCTAG ACAAACACTCGACTGAAAGCCAGCACACTGAGGACATGAATGGTGACCCGTCCCATGAAAGGTGCATCAG TAATATAAAGACCACTTCTACACCAGGAATCCTTGGAAATTCAGCATCAGGATCCACTTTCCAGAATGCT GAACAGACAGAGTCTACTGGCAGCAGTAAGCGCAGGGTGGCAGAGGAGTGCTTCCTCAGTGAGGACAACA AAAAGAGCAGAATAGATGTAGTGAGAAGAGGGGAAGAGCCCACAGCTAGGACAGAGGAAACCAGGCAAGC CAGAAGCCTGGGTGATAGACTCCAGGATGGAATGACCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 110377. Forward Primer - name:110377_F_cDNA_LOC238564, sequence:CCAGAATGACCAATTCTCTTCC; Reverse Primer - name:110377_N_SP6_cDNA_LOC238564, sequence:GTGGTCATTCCATCCTGGAGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15817 same embryo
	EMAGE:15816 same embryo
	EMAGE:15820 same embryo
	EMAGE:15818 same embryo
	EMAGE:15819 same embryo
	EurExpress:euxassay_013221 same experiment
	MGI:4822666 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source