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EMAGE:15820

Cntnap3 contactin associated protein-like 3 ( MGI:3588199)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820
"Pseudo-wholemount" of euxassay_013224. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013224_01 euxassay_013224_02 euxassay_013224_03 euxassay_013224_04
EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820
euxassay_013224_05 euxassay_013224_06 euxassay_013224_07 euxassay_013224_08 euxassay_013224_09
EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820
euxassay_013224_10 euxassay_013224_11 euxassay_013224_12 euxassay_013224_13 euxassay_013224_14
EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820
euxassay_013224_15 euxassay_013224_16 euxassay_013224_17 euxassay_013224_18 euxassay_013224_19
EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820 EMAGE:15820
euxassay_013224_20 euxassay_013224_21 euxassay_013224_22 euxassay_013224_23 euxassay_013224_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15820Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15820_wholemount_strong.wlz
15820_wholemount_moderate.wlz
15820_wholemount_weak.wlz
15820_wholemount_possible.wlz
15820_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15820_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon mantle layer
weak weak
regionalweak expression: see section 04 05 06 07 08 09 19 20 21 22 23
midbrain mantle layer
weak weak
regionalweak expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39574
Entity Detected:Cntnap3, contactin associated protein-like 3 ( MGI:3588199)
Sequence:sense strand is shown

>T39574
CTGTAATGACACCAGGAGTGGAGCCAGGATGTTCAGGTCACTGCAGCAGCTATGGACACCTGTGTCGAAA
TGGGGGAATGTGTCAAGAGAAACACAGAGGGATTGCTTGTGACTGTGCCTTCTCTGCCTACGATGGGCCA
CTGTGCTCATATGAGATTTCTGCATATTTTGAAACGGGTTCCTCAGTGACCTATAATTTTCAAGAATATT
CCACCCTGAGTGCAAACACCAGCTACCTGGCTTCTTCATTGCATGGGGATGTCATACTGTCCAGAGAAAC
CATCACGCTGAGCTTCCGAACCACACAGACACCATGCTTATTGCTTTATGTGACCTCTTTTTATGAGGAA
TACCTTTCCATTATCCTGGCCAACAATGGAAGTTTGCAGGTGAGGTACAAGTTGGACAGACATCGGAATG
CAGATGCCTTCAACTTTGACCTTAGAAACTTAGCAGATGGTCACTTTCACCAGCTGACGATTAACAGGGA
AGAAGCAGTAGTCTCTGTAGAGAGTACAAAGAGGCAAGTCATCTTGTCCTCAGGGACTGAATTCAATGCT
GTCAAATCGCTCGTATTGGGAAAGGTTTTAGAACCCCTGGATGCTGATCCTGAAACACAGCAGGCAGCCG
CTCAGGGCTTCACAGGCTGCCTCTCTTCTGTGAGATTTGGCCTGGAAGTCCCACTGAAGGCTGCACTGAA
TCAAAACCGGGCTAGAGTCACTATTCAAGGCAGTGTGGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 110422. Forward Primer - name:110422_F_cDNA_LOC238680, sequence:CTGTAATGACACCAGGAGTGGA; Reverse Primer - name:110422_N_SP6_cDNA_LOC238680, sequence:CAGCCACACTGCCTTGAATAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15815 same embryo
 EMAGE:15817 same embryo
 EMAGE:15816 same embryo
 EMAGE:15818 same embryo
 EMAGE:15819 same embryo
 EurExpress:euxassay_013224 same experiment
 MGI:4823966 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS