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EMAGE:15969

LOC434335 (LOC434335)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969
"Pseudo-wholemount" of euxassay_013407. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013407_01 euxassay_013407_02 euxassay_013407_03 euxassay_013407_04
EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969
euxassay_013407_05 euxassay_013407_06 euxassay_013407_07 euxassay_013407_08 euxassay_013407_09
EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969
euxassay_013407_10 euxassay_013407_11 euxassay_013407_12 euxassay_013407_13 euxassay_013407_14
EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969 EMAGE:15969
euxassay_013407_15 euxassay_013407_16 euxassay_013407_17 euxassay_013407_18 euxassay_013407_19
EMAGE:15969 EMAGE:15969
euxassay_013407_20 euxassay_013407_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15969Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15969_wholemount_strong.wlz
15969_wholemount_moderate.wlz
15969_wholemount_weak.wlz
15969_wholemount_possible.wlz
15969_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15969_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
vibrissa
weak weak
regionalweak expression: see section 08 09 21
hypothalamus mantle layer
strong strong
regionalstrong expression: see section 16 moderate expression: see section 08 09 10 13 14 15
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 10 11
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 03 04 moderate expression: see section 02 05 11 12 14
rest of cerebellum marginal layer
strong strong
regionalstrong expression: see section 03 04 13 14 15 16 moderate expression: see section 02 05 06 07 09 10 11 12
pons mantle layer
strong strong
regionalstrong expression: see section 04 15 moderate expression: see section 05 06
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40060
Entity Detected:LOC434335, (LOC434335)
Sequence:sense strand is shown

>T40060
CTAAACCACATTCTCCTCGTCCCGGCCGGCCGTCACCCTAGAGCAAGCACAGTTCTTCCTTCAGCTCCTC
TCCTGCTCACCATGGCTCAGCTGAAATCATTGCCTATGAGATAACCACTCACGGTTTCCCATCACCACAG
TGTTCTCATGGCCTGCCAAACGAGAACTTGCAGGCCTAGCATGTAAAGGGACCAGGGAAGGAGGCAGAGT
GGGGCCTGCTGTTGAGGCTGGCTGCTTCTGTGTTCTCTGCCTGTGCCAGGCTGCGTATGGTCTTCACTTG
CCGTCTTTGAACTGAGAGAACCTCAGCTGTAAGTGTGTTGGTGTGCGGGATGCTTCCCCAGAGGGATTCG
CTCTGCTCTGACAACACTTTGTCAATCTCGGGTAAAATGAGACCTTTCTCTTAAAGAGAAGGTTACAGCT
AATTTTCTGTAAGAGCAGTAAAGTTAAGAAGACCTTCCCTGAGGGTAGAAAAACCACAAAACCCAGGAGC
AGGGACTGTCTTCTTTCTTACAGTATTTACTATCCCCACCCCCACCCCTGCTGCCTTGCCATACCAGGCA
TGAAGGAACTAGTCACTTAAGCAAGAAAACTGTACTCCAGATCTTAAGAACCTCAATTCTCACCCTTATC
AGTGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109067. Forward Primer - name:109067_F_cDNA_LOC434335, sequence:CTAAACCACATTCTCCTCGTCC; Reverse Primer - name:109067_N_SP6_cDNA_LOC434335, sequence:GACACTGATAAGGGTGAGAATTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15968 same embryo
 EMAGE:15970 same embryo
 EMAGE:15967 same embryo
 EMAGE:15966 same embryo
 EMAGE:15971 same embryo
 EurExpress:euxassay_013407 same experiment
 MGI:4823548 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS