Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16138

Smok3c sperm motility kinase 3C ( MGI:3647925)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138
"Pseudo-wholemount" of euxassay_013567. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013567_01 euxassay_013567_02 euxassay_013567_03 euxassay_013567_04
EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138
euxassay_013567_05 euxassay_013567_06 euxassay_013567_07 euxassay_013567_08 euxassay_013567_09
EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138
euxassay_013567_10 euxassay_013567_11 euxassay_013567_12 euxassay_013567_13 euxassay_013567_14
EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138 EMAGE:16138
euxassay_013567_15 euxassay_013567_16 euxassay_013567_17 euxassay_013567_18 euxassay_013567_19
EMAGE:16138 EMAGE:16138
euxassay_013567_20 euxassay_013567_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16138Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16138_wholemount_strong.wlz
16138_wholemount_moderate.wlz
16138_wholemount_weak.wlz
16138_wholemount_possible.wlz
16138_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16138_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45342
Entity Detected:Smok3c, sperm motility kinase 3C ( MGI:3647925)
Sequence:sense strand is shown

>T45342
CTCCTGTGTGTATCCTGCATGTTGAACAAGGAGGGGAACTCACCATGCTAGGGGGAGGAAGTGGTGAGAA
GGAAGAGGAGGAGATGCTGAGGGAGGAGAGGATGGTATGTGATGGGAGCTAGGAGTGGGGGGAAGAGGTT
GGGACAGGAGGAGGCAACTTGGGGGAGCAGTGTGAAACAGGGTAACCACAGCTGGAGAGATGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 208867. Forward Primer - name:208867_F_exon_Smok2, sequence:CTCCTGTGTGTATCCTGCATGT; Reverse Primer - name:208867_N_SP6_exon_Smok2, sequence:GCATCTCTCCAGCTGTGGTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16140 same embryo
 EMAGE:16141 same embryo
 EMAGE:16139 same embryo
 EMAGE:16137 same embryo
 EMAGE:16136 same embryo
 EurExpress:euxassay_013567 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS