EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16141

Hcfc1r1 host cell factor C1 regulator 1 (XPO1-dependent) ( MGI:2663619)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013570. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013570_01	euxassay_013570_02	euxassay_013570_03	euxassay_013570_04

euxassay_013570_05	euxassay_013570_06	euxassay_013570_07	euxassay_013570_08	euxassay_013570_09

euxassay_013570_10	euxassay_013570_11	euxassay_013570_12	euxassay_013570_13	euxassay_013570_14

euxassay_013570_15	euxassay_013570_16	euxassay_013570_17	euxassay_013570_18	euxassay_013570_19

euxassay_013570_20	euxassay_013570_21	euxassay_013570_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16141_wholemount_strong.wlz

16141_wholemount_moderate.wlz

16141_wholemount_weak.wlz

16141_wholemount_possible.wlz

16141_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16141_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 07 08

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 06 07

facial vii ganglion

moderate

regional

moderate expression: see section 04 16 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 06 15

ventral grey horn

moderate

regional

moderate expression: see section 07 08 09 11 12 weak expression: see section 10

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45428
Entity Detected:	Hcfc1r1, host cell factor C1 regulator 1 (XPO1-dependent) ( MGI:2663619)
Sequence:	sense strand is shown >T45428 GATACCCCCAGTCCCTACTACCCTGCGTCCCCAGCTGGGGATATCGTGGAGCTCTGAGTATTGATGGCCT GTGTCCCTCCCTATCTTCTTTCTGACAACAAAGACCAGCAACTCCCAGAAAGGGATGAAGTCCTTCTGCA AACCTGGCCACCAGGACCTTCCCTCTGACAAGAGGACACTAGCTCGGTGGAACCCTCAGTGAAAGGCAGC CTGGGAAAGGGAAAATGTTACCCCATTAGTGAATAAAGACCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 193369. Forward Primer - name:193369_F_exon_Hcfc1r1, sequence:GATACCCCCAGTCCCTACTACC; Reverse Primer - name:193369_N_SP6_exon_Hcfc1r1, sequence:CAGGTCTTTATTCACTAATGGGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16140 same embryo
	EMAGE:16138 same embryo
	EMAGE:16139 same embryo
	EMAGE:16137 same embryo
	EMAGE:16136 same embryo
	EurExpress:euxassay_013570 same experiment
	MGI:4825332 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source