EMAGE mouse gene expression spatial database

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EMAGE:16211

Rmnd5b required for meiotic nuclear division 5 homolog B (S. cerevisiae) ( MGI:1913339)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017066. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017066_01	euxassay_017066_02	euxassay_017066_03	euxassay_017066_04

euxassay_017066_05	euxassay_017066_06	euxassay_017066_07	euxassay_017066_08	euxassay_017066_09

euxassay_017066_10	euxassay_017066_11	euxassay_017066_12	euxassay_017066_13	euxassay_017066_14

euxassay_017066_15	euxassay_017066_16	euxassay_017066_17	euxassay_017066_18	euxassay_017066_19

euxassay_017066_20	euxassay_017066_21	euxassay_017066_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16211_wholemount_strong.wlz

16211_wholemount_moderate.wlz

16211_wholemount_weak.wlz

16211_wholemount_possible.wlz

16211_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16211_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4167
Entity Detected:	Rmnd5b, required for meiotic nuclear division 5 homolog B (S. cerevisiae) ( MGI:1913339)
Sequence:	sense strand is shown >T4167 GGGCTGAGCTGCAAGACTAGGTGACAGCCTGCCGGCTCCCGGTCCCGGGGTGCGGGGCGCCCCGACCAAA GCCTGGAGCAGTCGTCGGCCACACCCTCCAAGGGCTGGTGGAGTCGCCCGGACTCCGACGTCCCGTCCTA GTGCACCTGACTGAGTCAGAGGATCCACACTGGCCTGACCCAGTTGACATCACCAGGCAGAGGCCGCCAT GGAGCAGTGTGCCTGTGTGGAGAGGGAGCTGGACAAGGTCCTGCACAAGTTCCTAACCTACGGGCAGCAT TGCGAGCAGAGTCTGGAGGAGCTGCTGCACTCCGTGGGCCAGCTGAGAGCCGAGCTGGCCAGTGCAGCTC TCCAGGGCACCCCTCTCTCAGCCACGCTGTCCCTTGTGATGTCCCAGTGCTGTCGGAAGATCAGAGACAC AGTCCAGAAACTGGCTTCCGACCATAAGGACATTCACAGCAGCGTCTCTCGAGTGGGCAAAGCCATTGAC AGGAACTTCGACTCTGAGATCTGCGGCGTGGTCTCGGATGCTGTGTGGGACTCCCGTGAGAAGCAGCAGC AGATCCTGCAGATGGCCATCGTAGAGCACCTGTACCAGCAAGGCATGCTCAGTGTAGCGGAGGAGCTGTG CCAGGAATCGACATTGAATGTGGACCTGGACTTCAAGCAGCCCTTCTTGGAGTTGAATCGGATCTTGGAA GCTCTGCACGAGCAAGACCTAGGACCTGCGCTGGAATGGGCTGTATCTCACAGGCAGCGCCTGCTGGAGC TCAACAGCTCGCTGGAGTTCAAGCTGCACCGGCTGCACTTTATCCGCCTCCTAGCTGGGGGCCCCGAGAA GCAGCTGGAGGCCCTCAGCTATGCTCGGCACTTCCAGCCTTTTGCTAGGCTACACCAGAGAGAGATCCAG GTGATGATGGGCAGTCTGGTGTACCTGCGGCTGGGCTTGGAGAAGTCACCCTACTGCCACCTCTTAGACA ACAGCCACTGGGCGGAGATCTGTGAGACCTTTACTCGGGATGCATGCTCCCTCCTGGGACTTTCAGTGGA GTCCCCACTTAGTGTCAGCTTCGCGTCTGGCTGTGTGGCGCTGCCCGTGCTGATGAACATTAAAGCTGTG ATCGAACAGAGGCAGTGCACTGGGGTGTGGAGTCACAAGGATGAGTTGCCGATTGAGATAGAGCTGGGCA TGAAGTGCTGGTACCACTCAGTGTTTGCATGCCCTATCCTGCGACAGCAGACCTCAGATTCCAACCCCCC CATCAAGCTCATCTGTGGCCACGTCATCTCCAGAGATGCACTCAACAAACTCATCAATGGAGGAAAGCTA AAGTGTCCCTACTGTCCCATGGAACAGAATCCAGCAGATGGGAAACGCATCATATTCTGATTCCAAGGAA CATTCTAGAAGGTGTAATCCACCCATGGGCCTTTGTGGAGGGACTGGCTTCAGCAGACTGTGATGCCTCT CAGACCAGCATTACCAAGAGACCCAACCTGCCTAGGCAGAGGCCAGGGAAGGCTGTGAACCAGCAGCCTA TGCTTCCTTACCTTCTAGGGGGACTGGCTACTCTGTTCCTGCATTCTGTTTACACTTGCCCCTGACGGAG CAGCAAAGGTCTTGGTCATCAAATGCAGACATCTTTCTACTCCTGGCATCAAAGGGGTTTCCAGCTGTGG CCCACTTCCTACTGTACAGTCTCAACTGTATCTACCCATTCATTCTCCACTGTAAATAACCCGTTAGAAC TGAATGCTGTTTTATAACTTTGAGCAAATATATTCATAGCCCTTCTCCTTAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4485143 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4485143 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16212 same embryo
	EMAGE:16209 same embryo
	EMAGE:16213 same embryo
	EMAGE:16210 same embryo
	EMAGE:16214 same embryo
	EurExpress:euxassay_017066 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source