EMAGE mouse gene expression spatial database

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EMAGE:16226

Mcee methylmalonyl CoA epimerase ( MGI:1920974)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017088. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017088_01	euxassay_017088_02	euxassay_017088_03	euxassay_017088_04

euxassay_017088_05	euxassay_017088_06	euxassay_017088_07	euxassay_017088_08	euxassay_017088_09

euxassay_017088_10	euxassay_017088_11	euxassay_017088_12	euxassay_017088_13	euxassay_017088_14

euxassay_017088_15	euxassay_017088_16	euxassay_017088_17	euxassay_017088_18	euxassay_017088_19

euxassay_017088_20	euxassay_017088_21	euxassay_017088_22	euxassay_017088_23	euxassay_017088_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16226_wholemount_strong.wlz

16226_wholemount_moderate.wlz

16226_wholemount_weak.wlz

16226_wholemount_possible.wlz

16226_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16226_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8314
Entity Detected:	Mcee, methylmalonyl CoA epimerase ( MGI:1920974)
Sequence:	sense strand is shown >T8314 CGCTGTGCAAGCCTGTAGCTTCTGGACATGAGGCGTGTAGTGAAGGCCGCCGCGTTGGCTGCGGGCGCTA CAGGGCTTTTCTCCAGAGTCCAAACTTCAGTTGCAATAGGGAGAAGTTTTTCCACACCACAGTCCCAGTT TCAAGAATCCAGTCCTGTGTGGAAGCTGGGTCGGCTCAATCACGTGGCTGTAGCAGTGCCAGATTTGGAA AAGGCCTCGTCATTTTACAGGGATGTTCTAGGGGCCCAGGTGAGTGAGGTGGTCCCTCTGCCGGAACATG GAGTATCTGTGGTTTTTGTCAACCTGGGAAATACCAAGATGGAACTGCTTCATCCACTGGGGAGTGATAG TCCAATCACAGGCTTTCTGCAGAAGAACAAGGCTGGAGGAATGCATCACGTCTGCATCGAGGTGGACAAC ATCAGTGCAGCTGTGATGGATCTGAAGAAGAAGAAGATCCGTAGTCTGAGTGATGAGGCCAAAATAGGAG CACATGGGAAACCTGTGATTTTCCTCCATCCCAAAGACTGTGGTGGGGTCCTTGTAGAACTGGAGCAAGC ATGATTTATAATTATGAGAAACTTAATTAATTGACCCAAGCAACCCTTATCAATATTATCAGATGTATTG TGCTACTACTTCCACTACTTTAAAACTTTAATGGCAGAAAGATTAAAATATATGCACATGTATATAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1479934 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1479934 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16225 same embryo
	EMAGE:16227 same embryo
	EMAGE:16224 same embryo
	EMAGE:16223 same embryo
	EMAGE:16228 same embryo
	EurExpress:euxassay_017088 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source