EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16228

Plin1 perilipin 1 ( MGI:1890505)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017090. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017090_01	euxassay_017090_02	euxassay_017090_03	euxassay_017090_04

euxassay_017090_05	euxassay_017090_06	euxassay_017090_07	euxassay_017090_08	euxassay_017090_09

euxassay_017090_10	euxassay_017090_11	euxassay_017090_12	euxassay_017090_13	euxassay_017090_14

euxassay_017090_15	euxassay_017090_16	euxassay_017090_17	euxassay_017090_18	euxassay_017090_19

euxassay_017090_20	euxassay_017090_21	euxassay_017090_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16228_wholemount_strong.wlz

16228_wholemount_moderate.wlz

16228_wholemount_weak.wlz

16228_wholemount_possible.wlz

16228_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16228_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8307
Entity Detected:	Plin1, perilipin 1 ( MGI:1890505)
Sequence:	sense strand is shown >T8307 GGCACGAGGCTGAGACTGAGGTGGCGGTCTGCTGCACGTGGAGAGTAAGGATGTCAATGAACAAGGGCCC AACCCTGCTGGATGGAGACCTCCCTGAGCAGGAGAACGTGCTCCAGAGAGTTCTGCAGCTGCCTGTGGTG AGCGGGACCTGTGAGTGCTTCCAGAAGACCTACAACAGCACCAAAGAAGCCCACCCCCTGGTGGCCTCTG TGTGCAATGCCTATGAGAAGGGTGTACAGGGTGCCAGCAACCTGGCTGCCTGGAGCATGGAGCCGGTGGT CCGTCGGCTGTCCACCCAGTTCACAGCTGCCAATGAGTTGGCCTGCAGAGGCCTGGACCACCTGGAGGAA AAGATCCCGGCTCTTCAATACCCTCCAGAAAAGATCGCCTCTGAACTGAAGGGCACCATCTCTACCCGCC TTCGAAGCGCCAGGAACAGCATCAGTGTGCCCATTGCAAGCACCTCTGACAAGGTTCTGGGGGCCACTCT GGCCGGCTGCGAGCTTGCCTTGGGGATGGCCAAAGAGACAGCAGAATATGCCGCCAACACCCGGGTTGGC CGACTGGCCTCTGGAGGGGCTGATCTGGCTCTGGGAAGCATCGAGAAGGTGGTAGAGTTCCTCCTGCCAC CAGACAAGGAGTCAGCCCCTTCTTCCGGACGGCAGAGGACCCAGAAGGCTCCCAAGGCCAAACCAAGCCT TGTGAGGAGGGTCAGCACCCTGGCCAACACTCTTTCTCGACACACCATGCAAACCACAGCATGGGCCCTG AAGCAGGGCCACTCTCTGGCCATGTGGATCCCGGGTGTGGCACCCCTGAGCAGCCTGGCCCAGTGGGGCG CATCGGCAGCCATGCAGGTGGTGTCCCGGCGGCAGAGTGAGGTGCGGGTGCCCTGGCTGCACAACCTGGC AGCCTCTCAGGATGAGAGCCATGACGACCAGACAGACACAGAGGGAGAGGAGACAGACGACGAGGAGGAG GAAGAAGAGTCCGAGGCTGAGGAGAACGTGCTCAGAGAGGTTACAGCCCTGCCCAACCCGAGAGGCCTCC TGGGTGGTGTGGTACACACCGTGCAGAACACTCTCCGGAACACCATCTCCGCAGTGACCTGGGCACCTGC GGCTGTGCTGGGCACGGTGGGAAGGATCCTGCACCTCACACCAGCCCAGGCTGTCTCCTCTACCAAAGGG AGGGCCATGTCCCTATCCGATGCCCTGAAGGGTGTTACGGATAACGTGGTAGACACTGTGGTACACTATG TGCCGCTTCCCAGGCTGTCCCTGATGGAGCCCGAGAGCGAATTCCGAGACATCGATAACCCTTCAGCAGA GGCGGAGCGCAAAGGGTCCGGGGCGCGGCCCGCCAGCCCGGAGTCCACCCCGCGCCCGGGCCAGCCCCGC GGCAGCTTGCGCAGCGTGCGGGGTCTCAGCGCGCCCTCCTGCCCCGGCCTGGACGACAAAACCGAGGCGT CAGCGCGTCCCGGCTTCCTGGCTATGCCCAGAGAGAAGCCTGCGCGCAGAGTCAGCGACAGCTTCTTCCG GCCCAGCGTCATGGAGCCCATCCTGGGCCGCGCGCAGTACAGCCAGCTGCGCAAGAAGAGCTGAGCAGAC TGCGCCCCTGCTCGCCCCACGGGAAGGTCGCTTCTCGTCAAGGGCCTTTCCTTTAGGGTGGCCTGAGCCA CGACCCCACGATGCTCTCGAGGCACACATCATGTCTAAAGCATCCTTTGGGGGCTTGACCATCAGAACCA ATTTTTAAGGGGCACCAGAGCCGCGGTCGACTCTCCTTTCTCGTGGGCAGATTCTCTCATGGCTTCTTCT TCCTCTTCTTTTTTTAAAAACTATAAAAGCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1448913 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1448913 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16225 same embryo
	EMAGE:16227 same embryo
	EMAGE:16224 same embryo
	EMAGE:16223 same embryo
	EMAGE:16226 same embryo
	EurExpress:euxassay_017090 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source