EMAGE mouse gene expression spatial database

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EMAGE:16335

Gm5478 predicted pseudogene 5478 ( MGI:3646318)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017175. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017175_01	euxassay_017175_02	euxassay_017175_03	euxassay_017175_04

euxassay_017175_05	euxassay_017175_06	euxassay_017175_07	euxassay_017175_08	euxassay_017175_09

euxassay_017175_10	euxassay_017175_11	euxassay_017175_12	euxassay_017175_13	euxassay_017175_14

euxassay_017175_15	euxassay_017175_16	euxassay_017175_17	euxassay_017175_18	euxassay_017175_19

euxassay_017175_20	euxassay_017175_21	euxassay_017175_22	euxassay_017175_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16335_wholemount_strong.wlz

16335_wholemount_moderate.wlz

16335_wholemount_weak.wlz

16335_wholemount_possible.wlz

16335_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16335_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5638
Entity Detected:	Gm5478, predicted pseudogene 5478 ( MGI:3646318)
Sequence:	sense strand is shown >T5638 CATTGTGTCTCTCCATATCCCATTTTCTTCTCTAGGAACCATGTCTACCAAATCCACCATCAGGATTCAA ACCAGCCACTGTGGCTTCAGTGCTAGCTCAGCCAGAGTACCTGGGCTCAACCGCTCTGGCTTCAGCAGTG TGTCCGTGTGCCGCTCCCGGGGCAGGGTGGCTCCAGTGCAATGTGTGGAGGAGCTGGCTTTGGCAGCAGG AGCCTCTATGGTGTGGGGAGCTCCAAGAGGATCTCCATCGGAGGGGGCAGCTGTGGCATTGGAGGAGGCT ATGGCAGCCGATTTGGAGGAGCCTATGGCAGCCGATTTGGAGGAAGCTTCGGCATTGGAGGTGGAGCTGG TAGTGGCTTTGGCTTCGGTGGTGGAGCTGGCTTTGGTGGTGGCTATGGGGGAGCTGGCTTCCCGGTGTGC CCACCTGGAGGCATCCAAGAGGTCACCATCAACCAGAACCTCCTCACCCCTCTGAACGTGCAAATCGACC CCACCATCCAGTGGGTCAGGACTGAGGAGAGGGAGCAGATCAAGACCCTCAACAACAAGTTTGCCTCCTT CATCGACAAGGTGCAGTTCCTGGAGCAGCAGAACAAGGTCCTGGACACCAAGTGGGCTCTGCTGCAGGAG CAGGGCACCAAGAACCAGAGGCCTGACTTGGAGACTCTGTTTGAGCAGTACATCAGCAACCTCCGCAGAC GACTGGACAGTATCACTGGAGAGAGGGGTCACCTGGATTCTGAGCTGAGAAAGATGCAGGACACAGCGGA GGACTACAAAGGCAAATATGAAGATGAAATCAACAAGCGCACAGCAGCAGAGAATGAATTTGTAACCGTG AAAAAGGATGTAGATGCTGCCTACATGACCAAGGTTGAACTGCAAGCCAAGGCAGATAGTGTTGCAGACG AGATCAACTTCCTGAGAGTTATTTTTGAGGCAGAACTGTCTCAGGTACAAACTGTCGTCTCAGACACAAC TGTGGTCCTCTCCATGGACAACAACCGTAGCCTGGACTTGGACAGCATCATTGCCGAGGTCAAGGCCAAG TATGAGGACATTGTTCAGAGAAGTCGTGCAGAAGCTGAGACTTTGTACCAGACTAAAGTGAGTGTTGGGA TGCAGGGCAATAAGGATGCCTGGGAACCACTCTAAACTCCATGAGTCTCTGAGTTCAGTCATGGAGACCC CTTAAAGAGAAGCTGGGGTGTTCTCACAGCAAATGTATTATGTCCTTGTGTTATGGACAGATGTTTTATT CTAAGAGAACAAGACCCTAGAGGCAGATGCAAAGTTAACATAAATGGTGTCCAGGGCTCTTTTTTCTTAT ACTCTGACCACGGATCCTCATGAGACACTAGAAAACGATTTCACTTCCTGAGGGAACAAAGGATTCTGTG GTCTACAGCTTGTCTTGCAGAAAAGCCATCACATCCATGGAAGTGTGTGCCACTTAGCGGTGGATTTGCT TCTGCATGTAACAACTCACATAAATTTTCTCTCTTTGTTTGGCCTTCAGTATGAGGAGCTACAGGTCACA GCTGGCAGACATGGGGACAACCTGCGCAACACCAAGCAGGAGATCGCTGAGATCAACCGCATTATCAGGA GGCTGAAAGCTGAGATTGACAGTGTTAAGAAGCAGCTTTCCAAACTGCAAGCCGAAATTGATGATGCTGA GAGACGTGGGGAGATGGCCCTGAACGATGCCAGGGGCAAGATGGAAGGGATGGAGGATGCCCTGCAGAAG GTCAAACAGGACACGGCCAGGATGCTGAATGAATACCAGGAGCTCATGAATGCCAAGCTGGGCCTTGATG TGGAAATCGCTGCCTATAGGGCAATTCTGGAAGGAGAGGAGTACAGGTGCGTAAGTACAACCTCCAGCCC CTGAGGACAGCTCCATGGTGCCAGCACTGAGCACAAGAAGAGGGAGCAGTGGCTGTGCTCACCATGGTTA CTTCAAGAAACTCCTTCCTGTGCTGGAGAGATGGCTCAGTGTTTAGTTCTTCTAGAGTTACTGACTTCAA TTCTCAGAAGCCATATGATGGCTCACTACCGTCTCTACTTGGATCAAGTTCCCTCTTCATGGGTGTCTAA AAACAGGTAGAGTGAACCCAAATACATAGAATAAATACAGGAGTATTAAAAAATAGATTTCTTTCCAGAA GATTCTGGACCTTCAACAGATTGAGGGTTCCCATTGGGACTGAGGTGATGGCTGCCTAGTTCTCACTTGC TGCTTTCCTCTCTTAGGTTCAGTGGAGAAGGTGCTGGACCAGTCAACACCTCTGTGAAGCAGCAGTCCAC CGTGTCCAGAGCCTATGGCAGCACAGGTGGTGCCAGCAGCAGCTTAGGCCCGGGCAGGGGCAGCAGCCAT TCCTATGCCAGTGTCCATGGTAGGTATGGCTTCAGTGCTGGAAGTGGCAGAGTCATCAGAGGTGGCCTCA ACTCCTCTGTTGGCAATCCTTCCACCATCAGTTCCAACCACCAGAGCTTGTTGTGGACCCCAAATAGCCT GGCTGTAATGCCCTGTGTACAGGGGCATCTCCTCCCTGCATCTCCTCCTTTGCTATCTGCTTGGGTGTGA GGAGGCTGTGTCCACTTGCTCCTCTTGGGATCTCACTGCTCATGTTTTGAGTCATCCTCTGAGCTCACAT CACGATTCACACACATTTTGTGCTTCATGCTGTAATTGGGTTTCTAGGCTCCGGCCTTCCTTCCTCTATC TCTGGGGTTCTTGTGAAAGGTTGTCTTTGTCTTCTTCATTCTGGAAATCATTGTCTTGTTCTTACTCAAG AAATGCACACGTCCCTTTGAGTTTCCAGCGGCCTGAGAAACCCCATCTTTCAACACCGGAAAATTCCCTG TACAGTCACTGGTCCTGTGATGTCTTTGCTCATGTGATGTTACCTCTGCTCTTTGCTCCCTTGTAATTGC TAAATAAAGCAGGTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6494053 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6494053 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16336 same embryo
	EMAGE:16337 same embryo
	EMAGE:16334 same embryo
	EurExpress:euxassay_017175 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source