EMAGE mouse gene expression spatial database

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EMAGE:16336

Rccd1 RCC1 domain containing 1 ( MGI:2444156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017172. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017172_01	euxassay_017172_02	euxassay_017172_03	euxassay_017172_04

euxassay_017172_05	euxassay_017172_06	euxassay_017172_07	euxassay_017172_08	euxassay_017172_09

euxassay_017172_10	euxassay_017172_11	euxassay_017172_12	euxassay_017172_13	euxassay_017172_14

euxassay_017172_15	euxassay_017172_16	euxassay_017172_17	euxassay_017172_18	euxassay_017172_19

euxassay_017172_20	euxassay_017172_21	euxassay_017172_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16336_wholemount_strong.wlz

16336_wholemount_moderate.wlz

16336_wholemount_weak.wlz

16336_wholemount_possible.wlz

16336_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16336_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5590
Entity Detected:	Rccd1, RCC1 domain containing 1 ( MGI:2444156)
Sequence:	sense strand is shown >T5590 CCCACGCGTCCGCCTCAGTCCTGCGCTTGTTCAGTAGTGAACGACCTCCTTCCTTGTTCAAAGGGAATGG AGGGTGTCAGGGCTTGGTCTTCGCCCGCCGTTTCCCTCGGATCGTCAAGAGTATCCATTCATTCAGTGGT TCCAACCGCGTCCACTGCTGTTGGCGCGCACAGGAGACTTCAGCCTACTGCCTGGTGGCAGGTAACCCGG CGCATGTGTGCCGGTCAGGCGCGCCCCAGTGGTGACGTCACCGCTCGTGCCTGCGATTGGCTCCGAAAAG CTGGCGCACCGAATGACCGCGTTGCTTGGCGCGGAGGCCCTTAAAAAGTGCTTTACTCCGCCGGCCATCT GTTCCCCGAGCCATACATTACTATGCGCTGCTCACTTTCAAGGTTTCCTGCTTAAAGGGCTTTCCTGATG AGTTGAGGGTACAGGGCTAATGGGAAGATGAAAGATTCCTTTGGTTGGGGATCCAGGAGCTGTAGTCTCA GATCTGACTTAAGAGGCTCGAGAGTGTCTGTAAAATTCTGATCCATTCGAGTGAAGTGGCGTTGCATTTT GAACCGCGCGACTGTCTCCCAATTTCAGCCTGAGCTCCCTGGGACCCATAGTCATCACATTAAGTCAAAG GAAAGGCGCAGCAAAGACTTCTTGAATAAACGACCGAGTAAGGTCCAGTGGAAATTGTCCAAATGCCTCT GAAACCCGCTGTACGCTGGATTTGAGCCAAAAGTAGAAGAAGTGGTCAGCTGCAGACAGTTCAGCAGTCA AAAATCTGGCCGGATTTGCCAGCGTTCCTAAGGTGTCCTGTTTGCAAGAGTCTCCTTGGTTTGCAACCTT AATCTTGCATCCCTTAGTCCACACCACAGCCAAGTGGCCACCCACAGAGGACTCAACCTGGACATGGCCG AGAAACGGCACGGAGCCTGGTTCGGCTTTGGTTTCTGTGGCTTTGGGCAAGCGCTGGGTTCTGGGAACAG CCACCATTCAGTGTACAGTCCCGAACCACTGCATGCCAGTGATGATATTTGCCAAGTGAGCGCAGGCTGG AGCTACACTGCCCTGGTGACCCGTGGAGGCCGGGTGGAATTGTCAGGTTCTGTGAGCGGTGCAGCAGACG GCTGCAGGGACGTGTGGGCTTCGGAGGAACTCCTGGTGCTGCTGCGCAACAAGGGCGGCGCCAGCACGGA ACTGCAGGCCTGGGTACCAGGTTCCGCGTTACAGGGGGAGCCCCTCTGGGTCCAGAACTTGGTTTCTGGG GCAAAGGGCCAAGGGGAAGATGAACCCAGCAGGGAGTCTAGGATGGGGACCCTGCCATTGCTACCCTGCG CCCGTGCCTATGTGACCCCAGAACCACCCTTCTGCCAGCCTCTGGCCCCAGAGCTGCGGGTCCGCCAGCT GGAGCTGGGAGCTGAGCATGTGCTGCTGTTGTGCACAGCTGGCCAGGTGTTCTCCTGGGGCGCGGGCAGG CATGGACAGCTGGGCCACGGGACGCTGGAGGCAGAACTAGAGCCAAGACTGTTGGAGGCACTGCAGGGCC TGCGGATGGCTAAGGTGGCTGCAGGTGGCTGGCACTCTGTGTGTTTGAGTGAAACGGGGGATATTTATAT ATGGGGCTGGAATGAGTCAGGGCAGCTGGCCCTGCCCACAAGGAGTGGGACAGAGAAGAAAGCAGAGAGA GAGGAAGCCACAGAATTGAATGAAGATGGTCTCAAAGAGGAATTAGCTGTGGCTGATGCAGGAGCTCCTG CCCACTTCATAGCCATCCAGCCCTTCCCTGCTCTTCTGGATCTTCCCCTGGGCTCAGATGCAGTTATGGC CAGCTGCGGATCCCGACACACAGCTGTGGTGACACGCACAGGAGAACTCTATACCTGGGGCTGGGGTAAA TACGGACAGCTTGGCCACAAGGACAGCACCAGCTTGGATCGACCCTGCTGTGTGGAGTACTTTGTAGAAA GACAACTTGAAGTAAGGGCTGTGACATGTGGACCCTGGAATACCTATGTCTATGCAATGGAAAGAGACAA AAGCTGAACTATCCCTTAGTGGATCCCCACTTATGTGCCTGGGTGAGAAAGAACTCTTGCTTCAACATGC TGTCCTAGGTGCATGGAAACCTAAGTTTTGAAACAAGGTTCACAGCGATCTCCTCTGCCTTGTAAGTGCT GGGATTAAAGGTGCATGATTTCAGAATAAGACAAAGCTTACAAGGCATGTATGAAAGAATTGAAGATATA TATATATAAATAAATATTTTTGTATGTAAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6487223 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6487223 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16335 same embryo
	EMAGE:16337 same embryo
	EMAGE:16334 same embryo
	EurExpress:euxassay_017172 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source