EMAGE mouse gene expression spatial database

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EMAGE:16360

Phf17 PHD finger protein 17 ( MGI:1925835)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017199. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017199_01	euxassay_017199_02	euxassay_017199_03	euxassay_017199_04

euxassay_017199_05	euxassay_017199_06	euxassay_017199_07	euxassay_017199_08	euxassay_017199_09

euxassay_017199_10	euxassay_017199_11	euxassay_017199_12	euxassay_017199_13	euxassay_017199_14

euxassay_017199_15	euxassay_017199_16	euxassay_017199_17	euxassay_017199_18	euxassay_017199_19

euxassay_017199_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16360_wholemount_strong.wlz

16360_wholemount_moderate.wlz

16360_wholemount_weak.wlz

16360_wholemount_possible.wlz

16360_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16360_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5007
Entity Detected:	Phf17, PHD finger protein 17 ( MGI:1925835)
Sequence:	sense strand is shown >T5007 CGCGCGGGCGACAGCAGGGGCCCGCCCGGCTCCCCGCGGGGCCTCGGTGGCCCGGACCCATGCGGCGCGG CGCGCAGCCCGCGGAGGCCCAGCCCGTGCGCAGCAGGCTGCCTGCTGTCTCCCGGGGAGATCATGAAACG AGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCTTGTCAACGACATGGTCCCAGCAT TCTCGATCCCAGCATGGGAGGAGTAGTACCTGCTCTAGACCTGAAGATCGAAAGCCTTCTGAGGTGTTTA GGACCGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAACCCGGATGACTACTACGTGCT GGCTGATCCCTGGAGACAGGAGTGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCAGGGACCATCCCACAG CCTGTGGCCAGGGTTGTGTCTGAAGAGAAGTCTCTCATGTTCATCAGGCCCAAGAAGTACATCGCGTCGT CCGGCTCTGAGCCCCCAGCGTTAGGCTACGTCGATATCCGGACTCTGGCAGACAGTGTGTGTCGGTATGA CCTCAATGATATGGATGCTGCGTGGCTGGAAGTAACCAATGAAGAATTTAAGGAGATGGGGATGCCGGAG CTGGACGAGTACACCATGGAAAGGGTCTTGGAGGAATTCGAACAGCGATGCTATGACAATATGAATCATG CCATAGAGACAGAAGAGGGCTTGGGGATTGAATACGACGAAGACGTTGTCTGCGATGTCTGCCAGTCACC TGATGGCGAGGACGGCAACGAGATGGTGTTCTGTGACAAGTGTAACATCTGTGTGCACCAGGCTTGCTAT GGGATCCTCAAGGTGCCAGAGGGCAGTTGGCTATGTCGTACATGTGCCCTGGGAGTTCAGCCAAAATGTT TGCTGTGTCCCAAGAAAGGCGGAGCTATGAAGCCGACCCGCAGCGGAACCAAGTGGGTCCACGTCAGCTG TGCCCTGTGGATCCCTGAGGTAAGCATTGGCAGCCCTGAGAAGATGGAGCCCATCACAAAAGTGTCTCAC ATTCCCAGCAGCCGGTGGGCGCTTGTGTGCAGCCTCTGCAATGAGAAATTCGGGGCCTCCATACAGTGCT CTGTGAAGAACTGCCGGACAGCCTTCCACGTGACTTGTGCTTTTGACCGTGGCCTGGAGATGAAGACCAT ATTGGCAGAGAATGATGAAGTCAAATTCAAGTCCTACTGCCCAAAGCACAGCTCACACAGGAAACCCGAG GAGGGCCTGGGTGAAGGAGCAGCCCAGGAGAATGGGGCCCCTGAGAGTTCTCCCCAGAGCCCTCTGGAGC CCTATGGTAGTCTGGAGCCGAATCGAGAGGAGGCCCACCGGGTGAGTGTTCGCAAGCAGAAGCTACAGCA GCTGGAGGATGAGTTCTACACCTTTGTCAACCTGCTGGATGTGGCCAGGGCGCTGCGGCTGCCTGAGGAA GTGGTGGATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGATCAACTTCAACAAGCCCCTCATCACCC CAAAGAAAGACGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTGTTTAGGAGGCTGCAGCTGTT CACGCACCTGCGGCAGGACCTGGAGAGGGTTCGGAACCTCACTTACATGGTGACCCGCAGGGAAAAGATT AAACGGTCTGTGTGCAAAGTCCAGGAACAAATTTTCACTCAGTACACTAAGCTCTTGGAGCAAGAAAAAG TTTCAGGTGTGCCTTCTTCCTGCTCCTCCGCACTGGAGAACATGCTTTTTTTCAACAGTCCTTCTGTGGG CCCCAATGCTCCCAAGATAGAGGACTTGAAATGGCATTCTGCATTCTTCAGGAAACAAATGGGCACTTCC TTGGTTCATCCACTGAAAAAGTCCCATAAACGAGATGCAGTGCAGAATAGTTCTGGGACCGAGGGCAAGA CCTCGCATAAGCAGCCAGGTCTTTGTGGTAGAAGGGAGGGGCTGGAGGTCTCAGAAAGCTTGCTGAGCTT AGAAAAGACTTTTGCAGAAGCACGTCTCCTATCATCAGCACAACAGAAAAATGGTGTGGTGACCCCAGAC CATGGGAAAAGAAGAGACAATCGTTTTCATTGTGATCTCGTTAAAGGAGACTTAAAGGACAAATCTTTTA AGCAGAGTCACAAGCCTCTCAGGTCCACAGACACATCCCAGAGGCATCTGGACAACACAAGAGCTGCCAC CTCCCCTGGAGTAGGGCAGTCAGCACCTGGCACCAGGAAGGAGATTGTGCCCAAGTGCAATGGCTCCCTA GTCAAAGTGCCTATAACACCTGCCAGCCCAGTGAAAAGCTGGGGAGGATTCCGGATTCCAAAGAAGGGGG AGCGGCAGCAGCAAGGAGAAGCCCATGATGGGGCCTGCCACCAGCACTCAGACTGCTCCCATCTGGGTGT AAGCCGAGCTCCAGCCAAGGAGAGAGCGAAGAGCAGGTTAAGAGCTGACAGTGAGAATGATGGGTACGCC CCTGATGGGGAAATGAGTGACTCAGAGAGCGAGGCATCAGAGAAGAAATGTATCCATGCCAGCAGCACCA TCAGCAGGAGGACAGATATCATCAGGCGAAGCATCTTGGCCTCTTGATGAAACAGCCGGTGTGGAAGCAC ATGGCTTGTTGCCGTGTGCTGAGGAGAGCTCTGATAATAGGGGCAAGCAAATACCCATTGTTTCTGAGCT ACAGACATACTTCCTTGCAATTCAGATTGAATTTTTTTCCAGAGTCATTTATAAATCATTGTGAGAAGTT TGTGTTATTTGCAACTTGTTGAGGAAACAGAAGAGTAGACTGTAACCATAAGACACTAAGATTAGAACCT GAACTAAACACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:5250231 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5250231 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16362 same embryo
	EMAGE:16361 same embryo
	EMAGE:16363 same embryo
	EurExpress:euxassay_017199 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source