EMAGE mouse gene expression spatial database

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EMAGE:16369

Lrrc40 leucine rich repeat containing 40 ( MGI:1914394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017174. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017174_01	euxassay_017174_02	euxassay_017174_03	euxassay_017174_04

euxassay_017174_05	euxassay_017174_06	euxassay_017174_07	euxassay_017174_08	euxassay_017174_09

euxassay_017174_10	euxassay_017174_11	euxassay_017174_12	euxassay_017174_13	euxassay_017174_14

euxassay_017174_15	euxassay_017174_16	euxassay_017174_17	euxassay_017174_18	euxassay_017174_19

euxassay_017174_20	euxassay_017174_21	euxassay_017174_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16369_wholemount_strong.wlz

16369_wholemount_moderate.wlz

16369_wholemount_weak.wlz

16369_wholemount_possible.wlz

16369_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16369_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6171
Entity Detected:	Lrrc40, leucine rich repeat containing 40 ( MGI:1914394)
Sequence:	sense strand is shown >T6171 GCCTGCTGATTTTCTTTGCTGTCCAGTCTGTTGCGACTCAATCTTTCCAGTAACCAGCTGAAGAATTTGC CAGCAGAAATAAGCAGAATGAAAAGATTAAAGCACTTGGATTGTGATGCCAATCTCCTGGAGACTGTGCC TCCTGATGTGGGTAGCATGGAATCACTGGAATTGCTTTATTTGCGGAGGAATAAATTGCGTGTTCTACCT GAATTTCCTTCTTGTAGGCAGCTAAAGGAATTACACCTAGCTGAAAACCAGATAGAGAAGTTAGGGGCAG AGCATCTTCAGCACCTGCAGGCCATCCTTGTCCTGGATCTAAGGGGCAATAAGCTGCGGTCTGTCCCTGA GGAAATGGCCCTGCTGCAGTCTTTGGAAAGGCTTGACCTGAGCAACAATGATATTAGTAGTCTGCCCTGC TCATTGGGAAATCTTCATTTGAAATTCCTGGCACTAGAAGGAAATCCTCTAAGAACCATTCGAAGAGAAA TTATAGCTAAAGGAACTCAAGAAGTTCTAAAATACCTGCGCAGCAAGATCAAAGATGATAGAACCAATCA AAATGATTCTGTTCCTGAGACTGCCATGACACTACCAAGTGAAGCCAGAGTCAATATCCATGCCATAGCC ACACTAAAGCTGCTAGATTATAGTGATAAACAAGCAACCTTGATTCCTGATGACCTATTTGATGCCACAA AAACGACCCTCATCACTTCCATCAACTTCAGCAAAAATCAGCTGTGCGAGATTCCACAAAGGATTGTTGA GCTGAAGGAAATGGTGTTGGATATCAACCTCAGTTTTAACAAGCTTTCATTTATATCCCACGAGTTATGC TTGCTTCAGAAATTGACATTTTTAGATTTAAGGAATAATTTTTTAAGTTCTTTGCCTGAAGAGATGTCAT CACTGACAAAACTGCAAACGATCAATCTTTCCTTTAACAGGTTCAAAGTGTTTCCTGAAGTTCTGTATCG AATCTCCACACTGGAAGCGGTTCTGATCAGTAACAATCAGGTTGGCTCTGTGGACCCTCAGAAGATGAAG CTGATGGAAAATCTGAATACTTTAGACCTTCAAAACAATGACCTTTTGCAGATTCCCCCAGAGCTTGGGA ACTGTGTGCAGCTACGGACACTACTGCTAGATGGAAATCCATTCCGAGTTCCTCGAGCGGCCATATTAAT GAAAGGCACAGCTGCTGTGCTGGAGTATTTGAGAGATCGAATCCCTGCCTGAGTACCGCTGGTTTCTTAC TCTGGGCATGGTCACTCGGGATAGGACAGAGCCAGCCTCCAGTGTCCAGGTGTCACTAGACAGTCACTGT TTGGGCTATGGTCGTTCCTACTAAATGTCTACAGTTTGTGAAGTGATGTGAACATTTATAATGTCAACTG CTACAGATTTTAAATGTTTTATAAATTCATTCCTTTATGACACTGCAAGAACTTTTCATATGATTTTTAT AATGAAAAAGCAACCATCTTTGTAAATCTGGGTTCCCCCTGGTATCTTTTCATTTTCCATCTTATATGAA CTGAGCAAGACTATATAAGGTGTAAAGTCAGCGAACTTTTACTAAATATTATTTTTTGGCATTTAAAAGA AAATCGGACTTTTCGTTGTCCAGGATAGGACTTGCTATGTGGTCAGTGTCAGTGCGTGTTTATGTTTAGG GTTTTGGTTTTGACTTTACATTTTTTTTTTTAATGTTTTCAGTGTCCTTTATTCCAATGTGATACATCTT ATTATTTCCACAACGTTGATTCTGAAATCTCCTTACAGTCTGATTAAAACTAGACTGGTAGTTTTACAGT AATTTTCTAAAAAGGAGTTAGATATAGCCAAGCTTGATTTAATAAAAGTCCAAATATAAACATTGTTGTT TACACTGAAATTATCAGATGCAGCTTTTCAGCCGTCCTGGTTCATCATGTGATTGAGTCGAGAGTAGCCA GCTCAGTAGAAGCATGTGTCAGCTCAGTGTCAGCCTGCGTGCGGAGGGTGGAGGTAGAGAACCTATAGGC GGTTGAGGTTGTTGATCTTAGGAAAATCAGTGCTAATAAAGATACATGCTTCGTGTTCATAATAAAAGCT ACATTTTTAAGGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497400 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497400 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16365 same embryo
	EMAGE:16366 same embryo
	EMAGE:16368 same embryo
	EMAGE:16367 same embryo
	EMAGE:16364 same embryo
	EurExpress:euxassay_017174 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source