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EMAGE:17042

Map4k1 mitogen-activated protein kinase kinase kinase kinase 1 ( MGI:1346882)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17042 EMAGE:17042 EMAGE:17042 EMAGE:17042 EMAGE:17042
"Pseudo-wholemount" of euxassay_008854. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008854_01 euxassay_008854_02 euxassay_008854_03 euxassay_008854_04
EMAGE:17042 EMAGE:17042 EMAGE:17042 EMAGE:17042 EMAGE:17042
euxassay_008854_05 euxassay_008854_06 euxassay_008854_07 euxassay_008854_08 euxassay_008854_09
EMAGE:17042 EMAGE:17042 EMAGE:17042 EMAGE:17042 EMAGE:17042
euxassay_008854_10 euxassay_008854_11 euxassay_008854_12 euxassay_008854_13 euxassay_008854_14
EMAGE:17042 EMAGE:17042 EMAGE:17042
euxassay_008854_15 euxassay_008854_16 euxassay_008854_17

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17042Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17042_wholemount_strong.wlz
17042_wholemount_moderate.wlz
17042_wholemount_weak.wlz
17042_wholemount_possible.wlz
17042_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17042_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3222
Entity Detected:Map4k1, mitogen-activated protein kinase kinase kinase kinase 1 ( MGI:1346882)
Sequence:sense strand is shown

>T3222
TGGCCTCGAGCAGATTCGGACGAGGCCTACCTGTATTCTCATAGCATCCTGGGCCTGCTTGAAAGGAAAG
ACGGCAGAACGGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTGGCAAGAAAGAACATGGTCTC
CAGTAAGATCCAGGACACCAANAGGCTGCCGGGCTTGCTGTGTGGCTGAGAGTGCTAGCTCTGGGGGCCC
ATTCTTATGTGGGGCATTGGAGACGTCTGTGGTTTTGCTCCAGTGGTACCAACCAATGAACAAATTCTTG
CTTGTCCGGCAGGTGCTGTTCCCGCTGCCCACGCCGCTGCCGGTGTTCACGCTGCTGACAACGCCGGGCT
CCGAGCTGCCAGCTGTGTGCATCGGCGTGAGCCCGGGGCAGGCGGCAAAGTCGGTGCTCTTCCACACCGT
GCGCTTCGGTGCGCTGTCCTGCTGGCTGGACGACTCCAGCACGGAGCACAAGGGACCAGTGCAGGGTGAT
CCAAGTAAAGGAANACATGGTGATGGTGN
Notes:The probe template was PCR amplified from IMAGE:2699103 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2699103 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17044 same embryo
 EMAGE:17040 same embryo
 EMAGE:17045 same embryo
 EMAGE:17041 same embryo
 EMAGE:17043 same embryo
 EurExpress:euxassay_008854 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS