EMAGE mouse gene expression spatial database

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EMAGE:17062

Ppm1h protein phosphatase 1H (PP2C domain containing) ( MGI:2442087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001457. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001457_01	euxassay_001457_02	euxassay_001457_03	euxassay_001457_04

euxassay_001457_05	euxassay_001457_06	euxassay_001457_07	euxassay_001457_08	euxassay_001457_09

euxassay_001457_10	euxassay_001457_11	euxassay_001457_12	euxassay_001457_13	euxassay_001457_14

euxassay_001457_15	euxassay_001457_16	euxassay_001457_17	euxassay_001457_18	euxassay_001457_19

euxassay_001457_20	euxassay_001457_21	euxassay_001457_22	euxassay_001457_23	euxassay_001457_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17062_wholemount_strong.wlz

17062_wholemount_moderate.wlz

17062_wholemount_weak.wlz

17062_wholemount_possible.wlz

17062_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17062_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 17 18 19 20 21

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15 16

cervical ganglion

moderate

regional

moderate expression: see section 07 08 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3246
Entity Detected:	Ppm1h, protein phosphatase 1H (PP2C domain containing) ( MGI:2442087)
Sequence:	sense strand is shown >T3246 TGGCCTCGAGCCAGATTCGGCACGAGGAGGATGATGACTTGAAGTTCCCCCTTATATATGGAGAAGGCAA GAAGGCCCGAGTAATGGCAACTATTGGAGTGACGAGGGGACTTGGGGACCATGACTTGAAGGTTCATGAC TCCAACATCTACATAAAACCATTCCTGTCTTCAGCTCCAGAGGTCAGAGTCTACGATCTCTCCAGATATG AGCACGGAGCAGATGACGTGCTGATCCTGGCTACTGATGGACTCTGGGATGTCTTATCCAACGAAGAAGT AGCGGAAGCAATCACTCAGTTTCTTCCTAACTGTGATCCAGACGACCCTCACAGATACACACTGGCGGCT CAGGACCTGGNGATGCGTGCCCGAGGCGTCCTGAAGGACCGAGGGTGGCGGATATCGAATGACCGACTGG GCTCAGGAGATGACATTTCTGTATATGTCATTCCTTTAATACACGGAAACAAACTGTCATGAAAGTGACC CAGGGGACTAGGAGGACAGAAGGGAAGAAAACTGGGGTGCCTACGAGCAGGGCGGG
Notes:	The probe template was PCR amplified from IMAGE:2779724 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2779724 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17065 same embryo
	EMAGE:17064 same embryo
	EMAGE:17066 same embryo
	EMAGE:17063 same embryo
	EurExpress:euxassay_001457 same experiment
	MGI:4827364 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source