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EMAGE:17066

H19 H19 fetal liver mRNA ( MGI:95891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17066 EMAGE:17066 EMAGE:17066 EMAGE:17066 EMAGE:17066
euxassay_001453_01 euxassay_001453_02 euxassay_001453_03 euxassay_001453_04 euxassay_001453_05
EMAGE:17066 EMAGE:17066 EMAGE:17066 EMAGE:17066 EMAGE:17066
euxassay_001453_06 euxassay_001453_07 euxassay_001453_08 euxassay_001453_09 euxassay_001453_10
EMAGE:17066 EMAGE:17066 EMAGE:17066 EMAGE:17066 EMAGE:17066
euxassay_001453_11 euxassay_001453_12 euxassay_001453_13 euxassay_001453_14 euxassay_001453_15
EMAGE:17066 EMAGE:17066
euxassay_001453_16 euxassay_001453_17

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17066Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17066_wholemount_strong.wlz
17066_wholemount_moderate.wlz
17066_wholemount_weak.wlz
17066_wholemount_possible.wlz
17066_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17066_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1258
Entity Detected:H19, H19 fetal liver mRNA ( MGI:95891)
Sequence:sense strand is shown

>T1258
AGACTGTTGGCCTACTGGAATCAAGAAGATGCTGCAATCAGAACCACTACACTACCTGCCTCAGGAATCT
GCTCCAAGGTGAAGCTGAAAGAACAGATGGTGTCAACATTTTGAAAGAGCAGACTCATAGCACCCACCCA
CCCCTGAGAATCCATCTTCATGGCCAACTCTGCCTGACCCGGGAGACCACCACCCACATCATCCTGGAGC
CAAGCCTCTACCCCGGGATGACTTCATCATCTCCCTCCTGTCTTTTTCTTCTTCCTCCTTTCCTGTAATT
CTGTTTCTTTCCTTTTGTTCCTTCCTTGCTTGAGAGACTCAAAGCACCCGTGACTCTGTTTCCCCATTTA
CCCCCTTTTGAATTTGCACTAAGTCGATTGCACTGGTTTGGAGTCCCGGAGATAGCTTTGAGTCTCTCCG
TATGAATGTATACAGCGAGTGTGTAAACCTCTTTGGCAATGCTGCCCCAGTACCCACCTGTCGTCCATCT
CCGTCTGAGGGCAACTGGGTGTGGCCGTGTGCTTGAGGCCTCGCCTTCCCCTCGCCTAGTCTGGAAGCAG
TTCCATCATAA
Notes:The probe template was PCR amplified from IMAGE:2225502 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225502 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17065 same embryo
 EMAGE:17064 same embryo
 EMAGE:17063 same embryo
 EMAGE:17062 same embryo
 EurExpress:euxassay_001453 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS