EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17093

Tesk2 testis-specific kinase 2 ( MGI:2385204)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001485. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001485_01	euxassay_001485_02	euxassay_001485_03	euxassay_001485_04

euxassay_001485_05	euxassay_001485_06	euxassay_001485_07	euxassay_001485_08	euxassay_001485_09

euxassay_001485_10	euxassay_001485_11	euxassay_001485_12	euxassay_001485_13	euxassay_001485_14

euxassay_001485_15

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17093_wholemount_strong.wlz

17093_wholemount_moderate.wlz

17093_wholemount_weak.wlz

17093_wholemount_possible.wlz

17093_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17093_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 09 10

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 08 10

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15

neural retina

moderate

regional

moderate expression: see section 01 02 15

esophagus

moderate

regional

moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4466
Entity Detected:	Tesk2, testis-specific kinase 2 ( MGI:2385204)
Sequence:	sense strand is shown >T4466 GAGAGTTTCCAGCTGCCTTCTCAGTCCCAGCGGTGATCCGCCTCCGGGCCCCGCCGAGTAGGCGGGGGAA AGCAGCTCAGACCGCAAGGCCATGGCAGTAGCCTCCAGCTCCTGCGCCGCCGCTGGCCCTGAGCAATCTC CGGCTTCTGCCCAAGGGACCCTCACCTCCTCCGCAGGCTAAACAGCCGCGGCCGGGAGCTGCTATGCTCG CTCTGGGCCGCTGCCTCCCTTACCCTCTGGCCGGCGCAGACGCCCCCTCCGCGGCTCAGTTGTGCCTCAG TGTCACATTGGAAAAGAAGCAAAATTGATCAGAAATTATGTGTGGAGTCTCTTCTTAAATGAAGAAGTGA TTATTGAGATTATGGATCGGAGCAAGCGGAACTCAATTGCAGGGTTTCCCCCACGTGTGGAGCGTCTGGA AGAATTTGAAGGAGGTGGTGGAGGGGATGGGAACACCGTGCAGGTGGGAAGAGTTTCATCATCTTCATAT CGTGCGATTATTAGTGCCTTTTCCAGACTGACGAGTTTGGATGACTTCACCCGTGAAAAAATAGGATCTG GGTTCTTCTCCGAAGTGTTCAAGGTGCGACACAGAGCTTCTGGTCAGGTGATGGCTCTCAAGATGAACAC GCTGAGCAGTAACCGAGCAAACCTGCTGAAAGAGATGCAGCTCATGAATAGACTCTCCCACCCCAACATC CTCAGGTTCATGGGTGTATGTGTACATCAAGGACAGTTGCATGCACTTACAGAGTATATCAACTCTGGGA ACCTGGAGCAGCTGCTGGACAGTGACTTGTATTTACCGTGGACTGTGAGAGTGAAACTGGCCTATGACAT AGCAGTGGGCCTCAGCTACCTTCACTTCAAAGGCATTTTCCATCGGGACCTCACATCAAAGAACTGCCTG ATAAAGAGAGATGAAAACGGTTACTCTGCTGTGGTAGCTGACTTCGGCCTGGCTGAGAAGATCCCTGACG CTAGTATAGGGAGGGAGAAGCTAGCTGTGGTGGGCTCGCCTTTCTGGATGGCACCTGAGGTTCTCCGAGA TGAGCCTTATAATGAGAAGGCAGATGTCTTCTCTTATGGTATCATCCTCTGCGAGATCATCGCCCGCATT CAGGCTGATCCGGACTATCTTCCCCGCACAGAGAATTTCGGGCTGGATTACGATGCTTTCCAGAACATGG TGGGAGACTGCCCCTCAGACTTCCTGCAGCTCACCTTTAACTGCTGTAATATGGACCCCAAACTGCGCCC ATCCTTTGAGGAGATTGGGAAGACCCTAAAGGAAATTATGAGCCGCTTACCGGAAGAAGAGTTGGAGAGA GATAGGAAGCTACAGCCCACAGCTAAAGGACCACTGGAGAAAGTGCCTGGAGGGAAGCGACTGAGTTCAC TGGATGACAAGATTCCCCACAAGTCTCCACGTCCAAGACGTACTATCTGGCTGTCTCGAAGCCAGTCAGA TATTTTTTCTCATAAGCCTCCACGTACAGTCAGTGTCTTGGACCCCTATTACCAGCCACGAGATGGTGCT ACACATACCCCAAAAGTCAACCCTTTCAGTGCACGCCAAGACCTCAAGGGCGGGAAGGTCAAATTCTTTG ACCTACCCAGCAAATCTGTCATTTCCCTTGTATTTGACCTGGATGCACCGGGGCCTGGAAGTACAACTCT GGCTGACTGTCAGGAGCCTTTGGCCATGTCTTCCAGACGGTGGCGGTCTTTGCCTGGTTCACCTGAGTTC TTGCATCAGGCTTGTCCATTTATGGGCTGTGAAGAATCACTCTCTGATGGGCCTCCACCACGACTCAGTA GTCTCAAGTATGGAGTAAGAGAGATCCCACCATTCCGGACATCTGCCCTATCAGCTGCTTCAGGCCACGA GGCCATGGACTGCTCTAACCCCCAAGAAGAGAACGGTTTTGGACCCAGGCTTAAGGGGACCAGCCTATGC ACTGGGGCTGCCTCTGAGGAGATGGAGGTGGAAGAGGAAAGGCCACGAAGAGCTTCTGTCTACTTCTCTA TCTCTGGAATAAGCTTGCAAACCCAGGCAAAGCAGGATGGGTGAGATGGGGGCAGGACTAGTCCCTGCCT GCCATATGAGAGGCGTTTCAGTAGTTAGGGCCAACCTAATTTTAAGCCTCTGAAATCCAGCAAGGAGGTT TGGTTCCCATGGACCACAGTGCACGTCCTAGACAGGACCAAAAAACTCTTCGTGCTAGGCTGGGCTGGCA GGTACCATGTACCAGTCCCTTTCCTACTCTTAAGTCTCTCATGCCGATTCTGGGGCATGTAAGCTACAGG ATGGAAACTGGAGTTGGCAGGAGGCCGCTAGGGTGTGGCTGTTTCTTAGACATGACGACTGGGATGCCTC TTGCCAGTGTGTCTCAGACACTTGAATAATTGCGGTTTGCACTTACTGTATGTCAGACCACATCACTACA TCTCTATGCAAGGGGGACAAGGCAATGTGGTGGTCGAGGATCTTTTAGCTAACCTAGTCAAAGACTTTTC CAGTTGATTAATCTATTTTCATATTTATAAAGGAGTCTTAATGTTTTGCCTCAAGACTTTCAACCTTGGC GTTGGGAGTGGGGCTGGTATTGTAGGCCCTAGGGCCTGCTCTATGTATGTGTCAACAGGTGATACAACCC TTTAGTTAATGGTTTATACTTGGACTGATTTTCTTTCCTCCTGTAGCTGGAGCTTTGGGAACAGTCTGTC CTTAAAGAGCCGCGATAAGAAATAACCAAAGAATGAAGTTGGTCAAATATTTTCATATTTCTGTTGATTT TTTTTTAAAACTTTTCCCAAAACACTTTCAGATTAAAAAAAATAATAAAGTTGATGTTGCAGGTGAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4947564 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4947564 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17094 same embryo
	EMAGE:17092 same embryo
	EMAGE:17096 same embryo
	EMAGE:17097 same embryo
	EMAGE:17095 same embryo
	EurExpress:euxassay_001485 same experiment
	MGI:4828664 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source