EMAGE mouse gene expression spatial database

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EMAGE:17097

Rnf122 ring finger protein 122 ( MGI:1916117)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001484. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001484_01	euxassay_001484_02	euxassay_001484_03	euxassay_001484_04

euxassay_001484_05	euxassay_001484_06	euxassay_001484_07	euxassay_001484_08	euxassay_001484_09

euxassay_001484_10	euxassay_001484_11	euxassay_001484_12	euxassay_001484_13	euxassay_001484_14

euxassay_001484_15	euxassay_001484_16	euxassay_001484_17	euxassay_001484_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17097_wholemount_strong.wlz

17097_wholemount_moderate.wlz

17097_wholemount_weak.wlz

17097_wholemount_possible.wlz

17097_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17097_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4465
Entity Detected:	Rnf122, ring finger protein 122 ( MGI:1916117)
Sequence:	sense strand is shown >T4465 GCGCGCACTGCTCAGCGCAGTGGGACCCAAGCCCGGCTTCGTGTCCTGGCTACTGCCTTCTCGGCCGGGC ATCCCGGCTCTCTCGGCTGCCCTCCCGGCCCGGGCCCTCGGTAGGGCTTCGCTCCGAAAAGACTTTGCTT CCTCCTCTGCCCGCTTTGTTGCTCTGCAAACCGGCCTCTCTGTAACCCTTGACTCCACTCCTCCGGGAGT TGCATCTCCATCCCGGCGGTCTCAGTGCACAGCCCCTCCAGCTCCAACACCTTGCCTCGGTTTGCTCCCG GAAGCCGACCTTGACTTCATTGATGCACCCATTCCAGTGGTGTAACGGGTGTTTCTGTGGCCTGGGACTG GTTAGTACCAACAAGTCCTGCTCAATGCCACCGATCAGTTTCCAAGACCTTCCCCTCAATATCTACATGG TCATCTTCGGCACAGGCATCTTCGTCTTCATGCTTAGCCTCATCTTCTGCTGCTACTTCATCAGCAAACT CCGGAACCAGGCTCAGAGCGAGCGATACGGCTACAAGGAGGTGGTGCTTAAAGGTGATGCTAAGAAGTTA CAGCTCTATGGGACCTGTGCAGTTTGCCTGGAAGACTTCAAGGGGAAGGATGAACTAGGCGTGCTTCCAT GCCAGCATGCCTTTCACCGCAAGTGTCTGGTGAAGTGGCTAGAAGTACGTTGTGTCTGCCCCATGTGCAA CAAGCCCATCGCGGGCCCCACGGAGACCTCGCAGAGCATCGGGATCCTGCTGGATGAACTGGTGTAAGCG CTCCTGGCACGTGGACACTGGGAAAGACTGCCGACTTTGGGTACCTGGGTTTCTCTACACAATGAACAAG ACTCACACACACCTAAAGGCAAGGATGACAGAGCCGAGCTTCTCCCAGCACCGCAGGACCAGTACCAGAC TTGTCCAGTCTCCGCCTCCCAGGGCTTCTCTCCGTTATCCATTTCCAATGGCCTCATCCAGGTAGACTGC TCTATCCTGGTACTGGACTGACCGTCCATCCAGCCATCTCCACCCACCTCATCTTTGCCCTGGGAAGGAG TCCACTGGGTGACTGTGACCATGGACGCCTCCCTTTGGACAACAAGCGACTCTGACCCTGAAAGGCTCTG CCCCACCCATGTCCTTCACTCCTGCCAGAGCCTTCCATGGGATGGTTTCCAAGGGAAAGGAGTGAAAGAT CAAGAAACCAAGACGAAAGGCTCTGTGTGAAGTCAAGGAAAGCAGGGCCCTAAAAAAGGGAAAAATCAAT GTTTACATGGGATGCCGGAAACAAAGGCCAGCCTCGCCCTGGCTGACTGCAGGGTGATGGGACCAGCCCT CCCCTGTTAGGATCTGAGGTGCTATTCATTCTCTCCCTGGCCCTGCTTAATCTCAGAGCCTTCTAGTCAT CGTTGGTTTGGAAGAGACCCCTCTCTATAGGAAGGGGTCTAATCCTCAAGCCCATTGTCAGGGGCTTTTT TTTTTTTTCTTTTTGGAAGCAAAACGGGCCTCGGGCTGGGGATGGTTGGGAGGATACCGTAGTGCGGATG TCTGCTTCCCCTCTGTAAATAGTATTTTTATACTTCATCTTCACCATCACCATCTCAGGCTTTACACGTG CAGCCCCTCCCCCACCCAGGATGGAGGGGGGGGTGGAGTGTCCCCTTCCTTCCCTGAACAGGGCGGCTGA GAGTGGGAAAAGGTTATGTATTTAAAGAAAATTAAATTTAATAACATGTTTCTCTAATCAGATTTTTGGC TTGTGGTTGTGGCCTGTGCCAGGAGAGGGAACAGAGCTCTTTACCTGCCTTCCCCCAGTCAGACCATACT TGAGGTTTCTAATCCTTAACAAAGCCTGAGGGCTGGACATCTATCTCAACAATAGAGTGCTTGGGTAACA TGCTCAAGGCCCTGGGCTCAGTTTTTGGAATGGAAAAAAGAAAAAAAAAAAAGCGGGGAGGGAGGGACGA TTAACTTATTGAACACTGGTATAAAATAAGATCTGATGAAGCCAGGATCAATCATCGGAAGTTTTAGAAA CATTGTAAAGTTGATTTCAAGCACAGCATTCACTCAACTGACTTGAATTCTACATGGTGGTTGATTGTCA GTGATCTGGCGACCTCAGAGGCCCATGTAGCTACCCTGGCTCCCCATCTCTTTTACTACTCCTGTGTCGC TGACAGTCCCTCCCTGGCACATCCTAGCCTCCTACACATACATGTAGAATTCCATGTGGTAGCAACAGGT ATGACCCTGCATGCATCCAGGTCCTCCACCCACAGCCTCCAGCTTAGCTTTGCCAGTTTCCCAAGGGGCC TGTTGTCAACAGTGAGTTCGAGCATAATGGGATGGTGTTGGGAACACCTTTCTTAAGCATAAGATAACTA GACCCTGGTTGGCCTTTGCTTGGGAGCACAGATTGATGCCAGTCAAGCAGACAGCACGCCTTGGGTGCTG AGCTCAAGAAAGCCATTTGCTGAGCATGGTTTGGATTGATGTCAGCTGTGAAAAAGCTACCCATCATTGA GTACTAGATACCTGGAGTAGGTATGGTGCTGTCTTACTCAGCCATTGTCTTGTGCATCTTGGACAACACT AAGGTGTAATTGGGTACCTGACTATGGGCTCTTCTACAGGAAGGTGCCTTTACTCTCTACATTAAGTGAC CCCTGCTTGCTATTGTCAACCTTAAGAGAAACAGATCTCTCTCCACTGGGTCACCACTTCCTCTTCTAGC AGTGATAGTCTGGAAGTATTGAGGAATTTTGATGTATTTTCTCAGCCGATTTCAGCCTACCCCTCAGTTC CTGCTGCAGGTGGATTCACTGGTGGAGAAAGTAATCAGGTTGGCTTTGTATCTCCCACCTCATCATCTCT CCTTGCAACTGGGAGGGTTCATCAGTGTTAGCATCCTGATGAAAAGGTTAGCACGAAAAAGCTTGTTTCA CAGCTTTCAAAACAGTGTTACCGGGTCAAAATGATGTGGGGGCAGGAAATACCTTTTATTCCCCGTGCTT CTATTGAACCTGGGTGGTATATATTACAGAGTACACAGAAGGGAAAATGATACTGGTTAGCTGATCAGCT CAACTGGCAGCCGCTAAGCACAATTAGACACTATGTCCCCAATTCACACAGTCACCGGTGAAGTCTTGTG ACTTGGTTAAGCCTTTTTAGAATACCTGCTGCCCGGTTTGGCCACTTGACAGGCAAATCCTGACCTTGGC GAAATGGTGTGAGCACTGTGCTTGGGTGGCAAGCTCACATAATCGAGATCTTTACTCAGCTGTCTTTTCA GCAGCAGCCCGAGTGTATATTGCCAAGACATGATGCTCAATCAGCAGCCCGAGTGTATGCCAAGACATGC TCAGTCTAGGGAGCAGTAATGTGGACATTGACTTTGATTAACCAGACTGTGGGGACCAGAGAGTTCAAAC CATTCTGTATGAACTGAGGTGGGAAAGACAGTAGGTTAGAACATAAAGGGAGGAAGTACTGTGTGGAAGC CAGGAACAGGATTGGATAGAGTAAGCACCCTGAACTTTATTTACATAAAACTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4947596 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4947596 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17094 same embryo
	EMAGE:17092 same embryo
	EMAGE:17096 same embryo
	EMAGE:17093 same embryo
	EMAGE:17095 same embryo
	EurExpress:euxassay_001484 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source