EMAGE mouse gene expression spatial database

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EMAGE:17138

Lemd2 LEM domain containing 2 ( MGI:2385045)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001525_01	euxassay_001525_02	euxassay_001525_03	euxassay_001525_04	euxassay_001525_05

euxassay_001525_06	euxassay_001525_07	euxassay_001525_08	euxassay_001525_09	euxassay_001525_10

euxassay_001525_11	euxassay_001525_12	euxassay_001525_13	euxassay_001525_14	euxassay_001525_15

euxassay_001525_16	euxassay_001525_17	euxassay_001525_18	euxassay_001525_19	euxassay_001525_20

euxassay_001525_21	euxassay_001525_22	euxassay_001525_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17138_wholemount_strong.wlz

17138_wholemount_moderate.wlz

17138_wholemount_weak.wlz

17138_wholemount_possible.wlz

17138_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17138_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4528
Entity Detected:	Lemd2, LEM domain containing 2 ( MGI:2385045)
Sequence:	sense strand is shown >T4528 GGGCCAGCCTGGGGCTGCTGCTCGGCTTCTTGGCCATCCTGTGGGTGAAGATGGGCAAGCCCTCGGCGCC GCAGGAGGCGGAGGACAACATGAAATTGTTGCCGGTCGACTGTGAGAGAAAAACAGATGAGTTCTGCCAG GCCAAGCAGAAGGCGGCCCTGTTGGAGCTGCTGCACGAACTGTACAACTTCCTGGCCATCCAGGCAGGTA ATTTTGAATGTGGCAACCCGGAGAAGCTGAAGAGCAAGTGCATCCCAGTCCTGGAGGCGCAGGAGTACAT AGCTAATGTGACCAGCAGTCCCTCTTCGAGGTTTAAGGCTGCGCTGACCTGGATCCTGAGCAGCAACAAG GACGTGGGCATCTGGCTGAAAGGGGAAGACCCATCTGAGCTGGCGACTACAGTGGACAAAGTGGTCTGCC TGGAGTCCGCCCGGCCCCGGATGGGCATAGGCTGCCGCCTCAGCCGCGCCCTGCTCACCGCTGTCACCCA CGTGCTCATCTTCTTCTGGTGCCTGGCCTTCCTGTGGGGGCTGCTGATCCTCCTGAAGTACCGCTGGCGG AAGCTGGAGGAGGAGGAGCAGGCCATGTATGAGATGGTGAAGAAGATCATAGATGTGGTTCAGGACCACT ATGTGGACTGGGAACAGGACATGGAGCGCTACCCCTACGTGGGCATCCTCCACGTGCGGGACAGTCTAAT CCCCCCACAGAGCCGGCGGCGCATGAAGCGAGTATGGGACCGTGCTGTGGAATTCCTGGCTTCCAATGAA TCTCGGATCCAGACTGAGTCCCACCGAGTGGCCGGGGAGGACATGCTGGTGTGGAGATGGACTAAGCCTT CCTCTTTCTCTGATTCAGAGCGATAGGGCAGGGCCTGTTACCGCCAGCCTGACCCAGCCACCCAGCTGGG GCAAGGACACAGACCTTTGGTGCTCATTCACACCTGCCTTGATTGCCCTCCGGGTCTCAGGTCTGGAACA TGTGGGCTTACTTAGGGTAGCCGGGAGAACAGGGGAGCCCTTCCTCTATGTCAGTATTACTGACTGACAG GGGAGAGTGGCTTGTTCTCCCTGGATAAAAAGATGGGCAGTGGTTGGAACCCTGGAGGTCCTGCACACAG CTAGACAGCACGGTGATCCTGAAGCCCCTCGCTTGGAAGGTGGGTTGGGTGGTGGGGCAAAGCTTGGTCC ATGTGCCTTTTCGCTTCCAGGGAGCTTAAGGGGAGGGGAGGAACCCAGCCCACTCCTGCATGGGCCTCTG TCCAGAAGGGGACGATGTTATAGAAGGTAGGAGCAGCTGGAGGGGACCAAGCAGAGGTGGCTGGGGTCTG GAAGGCGTGGGGAGTCCAGCCACAGCTGCCTGTGCCTCAGAGCTGCTCACAGGTCAGCAGTGCCACCTGA GCCAGGGTGGAGCTCAGCCTCCTCACCTACTTACTGTGCACAGGCTTCCTGGCTGCTTAGCCACACAGGT AGGGTGCAGCATCGTGGCTGCACACTGCCCTCTGCAGGCCACATGGAGTAGTGTGGGCCATTAGCCCTCG GCTATGGAGGACCTGCTCTAAATACACCCAGTGGGGGGCCTCATTAGCTGAGAGGGAGGCTTTCGAGCAA CTCTTCAGCCGGGTCTCACCTACAGCAGCAGGAGGACAGCCCCTGGAGGTGATGTGCAGCTGCCTCCAGA AGACACAAGACACCCCCTTTTGGACCTATTGCTGGTCTCTGTTTCTTAATAAAGTGTGGTCTCCATGTAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4972900 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4972900 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17139 same embryo
	EMAGE:17137 same embryo
	EMAGE:17140 same embryo
	EMAGE:17141 same embryo
	EurExpress:euxassay_001525 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source