EMAGE mouse gene expression spatial database

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EMAGE:17141

Gpc3 glypican 3 ( MGI:104903)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001524_01	euxassay_001524_02	euxassay_001524_03	euxassay_001524_04	euxassay_001524_05

euxassay_001524_06	euxassay_001524_07	euxassay_001524_08	euxassay_001524_09	euxassay_001524_10

euxassay_001524_11	euxassay_001524_12	euxassay_001524_13	euxassay_001524_14	euxassay_001524_15

euxassay_001524_16	euxassay_001524_17	euxassay_001524_18	euxassay_001524_19	euxassay_001524_20

euxassay_001524_21	euxassay_001524_22	euxassay_001524_23	euxassay_001524_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17141_wholemount_strong.wlz

17141_wholemount_moderate.wlz

17141_wholemount_weak.wlz

17141_wholemount_possible.wlz

17141_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17141_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

gland

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

visceral organ system

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lateral ventricle choroid plexus

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

4th ventricle lateral recess

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

cardiovascular system

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

skeleton

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4529
Entity Detected:	Gpc3, glypican 3 ( MGI:104903)
Sequence:	sense strand is shown >T4529 CTGGGTAGCGGCTCCTCTCTTGCTCTGTCGGGCTACTGCCAGACTTGCTGAGTCTCGGGACCGCTCCGGC TCTTATTGCCACTCTCTCGTGCTCTCCTCGCTCCCCCAAGAAGCAGGATGGCCGGGACCGTGCGCACCGC GTGCTTGCTGGTGGCGATGCTGCTAGGCTTGGGCTGCCTGGGACAGGCGCAGCCCCCGCCGCCTCCAGAC GCCACCTGTCACCAGGTCCGTTCTTTCTTCCAGAGACTGCAGCCCGGACTCAAATGGGTTCCAGAAACCC CTGTACCAGGATCAGATTTGCAAGTATGTCTCCCCAAGGGCCCAACATGCTGCTCAAGAAAGATGGAAGA AAAATACCAACTAACAGCACGGCTGAACATGGAACAACTGCTCCAGTCTGCGAGTATGGAACTCAAGTTC TTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCTTTGAAATTGTTGTTCGCCATGCCAAGAACTACA CCAACGCCATGTTCAAGAATAACTACCCCAGCCTGACTCCACAAGCTTTTGAGTTTGTCGGTGAATTTTT CACAGATGTGTCTCTCTACATCTTGGGTTCTGATATCAACGTGGATGATATGGTCAATGAATTGTTCGAC AGCCTCTTTCCAGTCATCTACACCCAGATGATGAACCCAGGCCTGCCTGAGTCAGTCTTAGACATCAACG AGTGCCTCCGAGGAGCAAGACGTGACCTGAAAGTATTTGGCAGTTTCCCCAAGCTTATTATGACCCAGGT TTCCAAGTCACTGCAAGTCACTCGAATCTTCCTTCAAGCCCTGAATCTCGGAATTGAAGTCATCAACACT ACCGACCACCTCAAGTTTAGTAAGGACTGTGGCCGTATGCTCACCCGAATGTGGTATTGCTCTTACTGCC AGGGACTGATGATGGTTAAGCCTTGCGGTGGTTATTGCAATGTGGTCATGCAAGGCTGTATGGCTGGTGT GGTGGAGATCGACAAGTACTGGAGAGAATACATTCTGTCTCTTGAAGAGCTCGTGAATGGCATGTACAGA ATCTACGACATGGAGAATGTGCTGCTCGGCCTCTTTTCTACCATCCATGATTCCATCCAGTATGTGCAGA AGAACGGAGGCAAGCTGACCACCACCATTGGCAAGTTGTGTGCCCACTCCCAGCAACGCCAATATAGATC TGCTTATTACCCTGAAGATCTGTTTATTGACAAGAAGATATTAAAAGTCGCTCATGTCGAACATGAAGAA ACCTTATCCAGCCGAAGAAGGGAACTGATTCAGAAACTGAAGTCTTTCATCAACTTCTATAGCGCTTTGC CGGGCTACATCTGCAGCCATAGCCCCGTGGCCGAAAATGATACCCTGTGCTGGAACGGACAAGAACTTGT GGAGAGATACAGCCAGAAGGCGGCAAGGAACGGGATGAAGAATCAGTTTAACCTCCATGAGCTGAAAATG AAGGGCCCTGAGCCGGTGGTTAGCCAGATCATTGACAAACTGAAGCACATTAACCAGCTCCTGAGAACCA TGTCTGTGCCCAAGGGTAAAGTTCTGGATAAAAGCCTGGATGAAGAAGGACTTGAAAGTGGAGACTGCGG TGATGATGAAGATGAATGCATTGGAAGCTCTGGTGACGGGATGGTGAAAGTGAAGAATCAACTGCGCTTC CTTGCAGAACTGGCCTATGATCTGGATGTGGACGATGCTCCGGGGAACAAGCAGCATGGAAATCAGAAGG ACAACGAGATCACCACCTCTCACAGCGTGGGGAACATGCCGTCCCCACTGAAGATCCTCATCAGTGTGGC CATCTATGTGGCGTGCTTTTTTTTCCTGGTGCACTGACTTGCCAGCGTCCAGTGCCTGTGCTGCCCTGCA GCACCTGTGGTCCCTACAGAAAGGGAGCCACCTTCTTTTTTTTTTCTTTTTTTTTTTTTTTTTATCTTTT ATGCCTCCTCCCACCACCATTAAGTAGGAGACTAACCGCGTGTTATGTTTTCGAAAATCAAATGGTATCT TTATGAGGATGGTAAATTTTAGTGGTAGGATAGATTGTCTTTTTGCAAAGAAAAAAAAAACCTTCAAGTT GTGCCAAATTATTTTCTTACATTTGACTGTTGGAACATGGTTGTCATGTTTCCCTCTTTTCTCTTTCTCT GCATGGATTTCTTTGACAAAAAAAAAATAAATAAACATTCAAATAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4973409 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4973409 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17139 same embryo
	EMAGE:17137 same embryo
	EMAGE:17140 same embryo
	EMAGE:17138 same embryo
	EurExpress:euxassay_001524 same experiment
	MGI:4825156 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source