EMAGE mouse gene expression spatial database

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EMAGE:17229

Clybl citrate lyase beta like ( MGI:1916884)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008876. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008876_01	euxassay_008876_02	euxassay_008876_03	euxassay_008876_04

euxassay_008876_05	euxassay_008876_06	euxassay_008876_07	euxassay_008876_08	euxassay_008876_09

euxassay_008876_10	euxassay_008876_11	euxassay_008876_12	euxassay_008876_13	euxassay_008876_14

euxassay_008876_15	euxassay_008876_16	euxassay_008876_17	euxassay_008876_18	euxassay_008876_19

euxassay_008876_20	euxassay_008876_21	euxassay_008876_22	euxassay_008876_23	euxassay_008876_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17229_wholemount_strong.wlz

17229_wholemount_moderate.wlz

17229_wholemount_weak.wlz

17229_wholemount_possible.wlz

17229_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17229_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 09 10 11 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13

diencephalon roof plate

strong

regional

strong expression: see section 12

thalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 14

choroid invagination

strong

regional

strong expression: see section 05 06 07 08 09 10 17 18 19

telencephalon mantle layer

strong

regional

strong expression: see section 11 12 13 14 15

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 02 03 04 05 06 07 10 17 18 19 20 21

midbrain lateral wall

strong

regional

strong expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4830
Entity Detected:	Clybl, citrate lyase beta like ( MGI:1916884)
Sequence:	sense strand is shown >T4830 CGTAGGCGGGCCAGTCGGGAAGATGGCGTTGTGCGTGCTGCGGAACACGGTTCGTGGAGCGGCGGCGCTG CCCAGGCTGAAGGCATCCCACGTGGTCAGTGTCTACAAGCCTAGATACAGCTCCTTGTCCAATCACAAGT ATGTCCCCCGGAGAGCAGTGCTCTATGTCCCTGGGAATGACGAAAAGAAAATAAGGAAGATCCCGTCCTT GAAAGTAGATTGTGCCGTGCTGGACTGCGAGGATGGTGTTGCAGAAAACAAAAAGAATGAAGCTCGATTG AGAATAGCAAAAACTCTTGAAGACTTTGACCTGGGCACAACGGAAAAGTGTGTGAGAATCAACTCGGTGT CTAGTGGTCTGGCTGAAGTCGACCTGGAGACATTTCTACAGGCCAGAGTTCTTCCCTCTAGTCTGATGCT ACCAAAGGTAGAAGGTCCTGAAGAAATCCGATGGTTTTCAGACAAATTTTCACTCCACTTAAAAGGCCGA AAACTTGAACAACCAATGAATTTAATCCCCTTTGTGGAGACTGCAATGGGTTTGCTCAATTTTAAGGCAG TGTGTGAAGAAACACTAAAGACTGGACCTCAAGTGGGTCTTTGCCTAGATGCAGTCGTTTTTGGAGGAGA AGATTTTCGAGCCAGCATAGGTGCAACAAGCAACAAAGACACCCAAGACATCCTCTATGCCCGGCAGAAG GTTGTTGTCACGGCAAAAGCCTTTGGCCTACAAGCCATAGATCTTGTGTACATTGACTTCCGAGATGAAG ACGGACTTCTCAGACAGTCAAGGGAAGCAGCCGCCATGGGCTTCACTGGTAAACAGGTGATCCACCCTAA CCAGATCGCAGTGGTACAGGAACAGTTTACTCCAACCCCAGAAAAAATTCAGTGGGCTGAAGAGCTGATT GCTGCCTTCAAAGAACACCAGCAGCTGGGAAAGGGAGCCTTTACTTTCCGAGGAAGTATGATCGACATGC CATTACTGAAGCAGGCCCAGAACATTGTTACGCTTGCCACATCCATCAAGGAAAAATGATCTGTTAAATG AAGCCCTCCTCAGGCGGGCTGAATGGATACTGTGGGCTTCTTAAAGACAAACCGCAGTACCCAGGGCAGC TTGCTAGCATGCTGGATCAGGACCTCATTTCACGTTAGTCACAACGCCCCCTCCCCTTGTATCTTACTGA CCCGCTATGCTAAAAATAAACTTTCTTCCTCTGGAGTCTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5064709 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5064709 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17228 same embryo
	EMAGE:17230 same embryo
	EMAGE:17227 same embryo
	EMAGE:17231 same embryo
	EurExpress:euxassay_008876 same experiment
	MGI:4823941 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source