EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17276

Thyn1 thymocyte nuclear protein 1 ( MGI:1925112)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003677_01	euxassay_003677_02	euxassay_003677_03	euxassay_003677_04	euxassay_003677_05

euxassay_003677_06	euxassay_003677_07	euxassay_003677_08	euxassay_003677_09	euxassay_003677_10

euxassay_003677_11	euxassay_003677_12	euxassay_003677_13	euxassay_003677_14	euxassay_003677_15

euxassay_003677_16	euxassay_003677_17	euxassay_003677_18	euxassay_003677_19	euxassay_003677_20

euxassay_003677_21	euxassay_003677_22	euxassay_003677_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5687
Entity Detected:	Thyn1, thymocyte nuclear protein 1 ( MGI:1925112)
Sequence:	sense strand is shown >T5687 GCTCCCAGAAGATTAGCCTGGCAGTGCTTCAGGCGTTTGGGTGGTTTTAGAAGACAAGAGCTGTTTCTTA CCCGTCCGGTTAGCTTCAGCCTCCTACAGAGAGGCCATGCCGAGACCCCGCAAGAGGCAGACTGGGACTG CTGGTCCAGACAGGAAGAAGCTATCCGGGAAACGTACTAAAACTGAAAACTCAGAAAGTACATCAGTCAA GCTAGAGAACTCCAGCCTTGAGATGACGACAACCTTTAAAAATGGTGGCAAGAATTTAAGCAACTACTGG CTGATGAAGTCAGAGCCAGAAAGCCGGCTAGAGAAAGGTATAGACATGAAGTTCAGCATCGAGGATCTCA AAGCACAGCCCAAGCAGACAGCTTGCTGGGATGGTGTTCGCAACTATCAGGCTCGGAACTTCCTCAGGGC CATGAAATTGGAGGATGAAGCCTTCTTCTACCATAGCAACTGCAAACAGCCTGGCATTGTTGGACTTATG AAGATTGTAAAGGAGGCTTACCCAGACCATACACAGTTTGAGAAAAGTAACCCCCATTACGATCCATCCA GCAAAGAGGACGACCCTAAATGGTCGATGGTGGATGTACAGTTTGTTCGAATGATGAAGCGCTTCATCCC TCTGGAGGAGCTTAAAACCTATCACCAAGCCCACAAAGCTACTGGCGGCCCCTTAAAAAGCATGACTCTC TTCACCCGCCAGAGACTTTCCGTTCAGCCTCTGACTCAAGAAGAGTTTGATTTTATTTTGAGCCTGGAGG AAACAGAACCAAGTTAATCAGAAGGCTCTACTGATGGAGTGAACAAGCCAAGATTTTATAAGACAGAAGG AGAAAGCTTGCTGTATCCACCTGGGGTCGTGTGCACGGATGGTTTGTTAATTTTCCTAATAAAAGATTTA ATGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2631627 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2631627 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17273 same embryo
	EMAGE:17272 same embryo
	EMAGE:17271 same embryo
	EMAGE:17274 same embryo
	EMAGE:17275 same embryo
	EurExpress:euxassay_003677 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source