EMAGE mouse gene expression spatial database

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EMAGE:17363

Phf1 PHD finger protein 1 ( MGI:98647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003823. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003823_01	euxassay_003823_02	euxassay_003823_03	euxassay_003823_04

euxassay_003823_05	euxassay_003823_06	euxassay_003823_07	euxassay_003823_08	euxassay_003823_09

euxassay_003823_10	euxassay_003823_11	euxassay_003823_12	euxassay_003823_13	euxassay_003823_14

euxassay_003823_15	euxassay_003823_16	euxassay_003823_17	euxassay_003823_18	euxassay_003823_19

euxassay_003823_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17363_wholemount_strong.wlz

17363_wholemount_moderate.wlz

17363_wholemount_weak.wlz

17363_wholemount_possible.wlz

17363_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17363_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7036
Entity Detected:	Phf1, PHD finger protein 1 ( MGI:98647)
Sequence:	sense strand is shown >T7036 CCACGCGTCCGCTTCCCGCACCGGAGACTCATCCAGGTCTCTTGAGTCTGACCCTACCTGGCTTCCAGCT GCCCCGGCTGTCACCGGCCCCCCCAGGATGCAATGGCGCAGCTCCCCCGGCTGAGCCGTTTGGGTGCCCC CTCTCTTTGGGATCCAGCTTCCCCTGCTCCCACCTCAGGCCCCAGACCTCGGCTTTGGGAAGGCCAAGAT GTGCTGGCCAGATGGACTGATGGGCTGCTGTACTTGGGTACCATCAAAAAGGTGGACAGTGCTCGAGAGG TGTGTCTGGTCCAGTTTGAGGACGATTCCCAGTTTCTGGTTCTATGGAAGGATATCAGCCCAGCTGCCCT CCCTGGGGAGGAGCTGCTCTGTTGTGTGTGTCGCTCTGAGACCGTGGTCCCTGGGAACCGGCTGGTCAGC TGTGAGAAGTGTCGCCATGCTTATCACCAGGACTGTCACGTTCCCAGGGCCCCGGCCCCTGGAGAAGGAG AGGGCGCATCCTGGGTATGCCGCCAGTGTGTCTTTGCAATCGCCACTAAGAGGGGAGGCGCACTGAAGAA AGGTCCCTATGCCCGGGCCATGCTGGGCATGAAGCTCTCCCTGCCATACGGATTAAAAGGGCTGGACTGG GATGCTGGACATTTGAGCAACAGGCAGCAGAGCTACTGCTACTGTGGAGGCCCTGGAGAGTGGAACCTGA AAATGCTGCAGTGCCGGAGCTGCCTCCAGTGGTTCCATGAGGCCTGCACCCAGTGTCTGAGCAAGCCCCT CCTCTACGGAGACAGATTCTATGAATTTGAGTGCTGTTGTGCCGGGGCGGCCCTGAGAAGGTCCGGAGGT TACAGCTTCGCTGGGTGGATGTGGCCCATCTTGTCCTCTACCACCTCAGCGTTTGCTGTAAGAAGAAATA TTTTGATTTTGACCGAGAGATCCTCCCCTTCACCTCTGAGAATTGGGACAGTCTGCTCCTGGGGGAGCTC TCAGATACTCCCAAGGGAGAGCGCTCTTCCCAGCTCCTTTCTGCTCTTAACAGCCACAAGGACCGTTTCA TTTCAGGGAGGGAGATTAAAAAGCGAAAATGTCTTTTTGGTCTCCATGCTCGGACCCCTCCTCCTGTGGA GCTTCTCACTGGAGACGGAGCCCCCACCAGCTTCCCTTCAGGGCAGGGCCCTGGGGGAGGGGTCTCACGT CCCCTGGGGAAACGATGGAGGTCGGAGCCAGAACCCTTAAGGAGGAGGCAGAAGGGGAAAGTGGAGGAAC TGGGGCCACCCACAGCAGCGCACAGTCGGCATGGGTCCCGGGAGCAGAGGGCCCTGCAGGCCTCGGTGTC TCCACCACCCCCCAGCCCTAACCAGAGCTACGAGGGCAGCAGCGGCTACAACTTCCGGCCCACAGACGCC CGCTGTCTGCCCAGCCCCATCCGGATGTTCGCCTCCTTCCACCCTTCTGCCAGCACTGCAGGGACCTCTG GGGACAGTGAACCCCCAGATAGGTCACCTCTGGGACTTCACATTGGCTTCCCCACAGACACCCCTAAAAG TTCACCCCACTCAGTGACGGCCTCATCTTCCTCGGTCCCAGCCCTGACCCCAGGCTTTTCCAGACATTCA CTACCCCCTTCTCCCTTGTGCCGTAGTCTGTCTCCGGGGACTGGGGGAGGAGTCCGAGGTGGGGTTAGCT ACCTGTCCCGAGGGGACCCTGTGAGGGTCCTTGCTCGAAGAGTGCGGCCTGACGGCTCTGTGCAGTACCT AGTTGAGTGGGGAGGGGGGGGCATCTTCTGACAGCCTGCTTCTGCTCCCCTCCCCATTCACACACTGGCA CTTTCATGCCCTGACCTCTGACCTCACCTACAGCTGGGATGTACCTGGAGAGATGGGGAGTGGTTCTCCC TCCTGCCCAGGCTGGAATCCAGGGGAGTGAAGAGGTCCTCTTCTCTGCCCCTTCATGACTCCTAATGCCC CCCCTCATTTCCTTTGATGTTATTTTGTTACAGCTTTTGAGATATTTTTTAAGACTATTTAACCCCTGGG ATTAGAGATTGGGGGGGGGGGGTTGGGTGGTCCAGGATTCCAGACTGTATGACTGTAATAAAGATAAATA AACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4007482 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4007482 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17360 same embryo
	EMAGE:17362 same embryo
	EMAGE:17359 same embryo
	EMAGE:17358 same embryo
	EMAGE:17361 same embryo
	EurExpress:euxassay_003823 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source