EMAGE mouse gene expression spatial database

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EMAGE:17391

Wdsub1 WD repeat, SAM and U-box domain containing 1 ( MGI:1919387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003839. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003839_01	euxassay_003839_02	euxassay_003839_03	euxassay_003839_04

euxassay_003839_05	euxassay_003839_06	euxassay_003839_07	euxassay_003839_08	euxassay_003839_09

euxassay_003839_10	euxassay_003839_11	euxassay_003839_12	euxassay_003839_13	euxassay_003839_14

euxassay_003839_15	euxassay_003839_16	euxassay_003839_17	euxassay_003839_18	euxassay_003839_19

euxassay_003839_20	euxassay_003839_21	euxassay_003839_22	euxassay_003839_23	euxassay_003839_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17391_wholemount_strong.wlz

17391_wholemount_moderate.wlz

17391_wholemount_weak.wlz

17391_wholemount_possible.wlz

17391_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17391_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7768
Entity Detected:	Wdsub1, WD repeat, SAM and U-box domain containing 1 ( MGI:1919387)
Sequence:	sense strand is shown >T7768 TGAGTCGGGCTCTGCCTAGTGAGCAGCCCAGCCCAGCAGCCCAGCTCGGGGCCCAGCCCGGGGCCGAGCC CGGGGCAGTGCAGACTTGGTGACCTCAGAACCCGGAGGTGCGTGACCTCGACCCCGGAGCCGCGCAGGGG AGGAGCCGCCCGGAGGCGGGACTCTCAGTCCGGGTGCGAGGGAAGCTGTTTACTTTGTGTGAGGAACATG GTGAGGCTGATTCACACGCTGGCTGATCACGGTGATGACGTCAGCTGCTGCGCCTTCTCGGCTGCCCTCC TGGCCACCTGCTCCTTGGACAAGACCATCCGCCTGTACTCCCTAAGTGACTTTGCTGAACTGCCGTACTC CCCGCTGAAGTTCCACACCTATGCTGTCCACTGCTGCTGTTTCTCACCCTCAGGACACGTTTTAGCATCG TGCTCGACAGACGGGACCACGGTGCTGTGGAGCTCGCACAGCGGACACACCCTGACCGTGTTGGAGCAGC CGGGTGGCAGCCCTGTGCGCGTCTGTTGCTTTTCCCCAGACTCTGCCTACCTAGCGTCAGGGGCTGCCGA TGGATCCATTGCTTTGTGGAATGCACAGACATACAAACTATATAGGTGTGGTAGTGTCAAGGATAGCTCA TTGGTGGCCTGTGCGTTTTCTCCCGATGGAGGCCTCTTTGTCACTGGCTCCTCGGGCGGGGACTTGACAG TGTGGGATGACAGAATGAGGTGTCTACACAGCGAGAAGGCGCACGATCTCGGGATCACCTGCTGCAGCTT TTCCTCACAGCCTCTCTCTGGCGGAGAAGGCCTCCAGTCTTACCAGTTGGCGTCATGTGGTCAAGACTGT GAAATCAAACTCTGGGCTGTTACTATTACCCGTGTCTTAGGCTTTGAATTAAAATATAAAAGCACACTAA GTGGGCACTGCGCCCCTGTTCTGGCCTGTGCTTTTTCACATGATGGAAAGATGCTTGCATCGGGGTCAGT GGATAAATCTGTCATCATACATGGTATCGGCCCTCAGAGTGTGCTACACACGCTGACTCAGCATACCAGG TATGTTACGACTTGTGCGTTTGCACCCAACACTCTCTTACTTGCTACTGGTTCAATGGACAAGACAGTGA ACATTTGGCAGTTTGACCTGGAAACACCTTGCCAAGGAAGCATGAACGACCCGCTGAAACATTTCACTGA AGAATGGTCAGAGGAGGATGTCTCCGTGTGGCTTCGTGCTCAAGGCTTGGAAGACCTCGTCGGTATTTTC AGGGCAAACAACATCGATGGGAAAGAACTATTGCATCTCACAAAGGAAAGTCTGGCTGGTGATTTGAAAA TCGAATCTCTAGGGCTGCGCAGCAAAGTCCTGAGGAGTATTGAAGAGCTCAGGGCCAAGATGGATTCCCT CTCTTCCGGAATCCCTGACGAGTTCATCTGCCCAATAACCAGAGAACTCATGAAGGACCCCGTCATCGCA TCAGATGGCTACTCCTACGAGAGAGAAGCAATGGAAAGCTGGATCCACAAGAAGAAGCGTACGAGCCCCA TGACAAATTTGGCTCTCCCTTCACTGGTACTGACCCCAAACAGGACACTGAAGATGGCCATCAACCGATG GCTGGAGACGCACGAGAAGTGAACGCGTTCACAGGCATCGGATCCACTTTCAGTGATGCCCTGCAAATGA TTCAAAATGCTAAGCAGCCATCACGAAAGCAAAATAAAAGGAAAAGACAAATGTTCAATTCAGTTACTTT TAAAAACTGTAAATTATGAGCAGGGCAGTGGTGGTGCCCACCTTTAATCCCAGCACTCAGGAGGCAGAGA CAGGTGGATCTCCAGGATCAGGAGTTCCAGGACAGCCCAGTTTATAGGGCAAGTCTCAGGACGGCCAAGG CTACACAGAGAAACCCTGTCTCAAAAAAACCCAAAACCAAAAAAAAAAAAAAAAAAAGTCAATTATCTTT AAAACACAGATTTATATATCTATTGTCATTGCTATTTCTGTAAAGGTGAAAATATTTTTTTTTTGCAATA ATGAGAAACTATGTAGAAATAAAACTTCACTATGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312489 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312489 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17392 same embryo
	EMAGE:17393 same embryo
	EMAGE:17390 same embryo
	EurExpress:euxassay_003839 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source