EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17393

Nxn nucleoredoxin ( MGI:109331)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003834. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003834_01	euxassay_003834_02	euxassay_003834_03	euxassay_003834_04

euxassay_003834_05	euxassay_003834_06	euxassay_003834_07	euxassay_003834_08	euxassay_003834_09

euxassay_003834_10	euxassay_003834_11	euxassay_003834_12	euxassay_003834_13	euxassay_003834_14

euxassay_003834_15	euxassay_003834_16	euxassay_003834_17	euxassay_003834_18	euxassay_003834_19

euxassay_003834_20	euxassay_003834_21	euxassay_003834_22	euxassay_003834_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17393_wholemount_strong.wlz

17393_wholemount_moderate.wlz

17393_wholemount_weak.wlz

17393_wholemount_possible.wlz

17393_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17393_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22 23

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

organ system

moderate

regional

moderate expression: see section 18 20 21

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 22 23

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

respiratory system

moderate

regional

moderate expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22 23

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tail

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 not examined expression: see section 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7772
Entity Detected:	Nxn, nucleoredoxin ( MGI:109331)
Sequence:	sense strand is shown >T7772 CGCCCGCCGCGCGGACCAGCGAGCCGGTAGCTGTTGCGAGCCCACCCGCCCGCCCGCGTGCCGGGGCTGC GGTGGGCCAGGATGTCGGGCTTCCTGGAGGAGCTGCTGGGAGACAAGCTGGTGACGGGCGGTGGCGAGGA GGTGGACGTGCATTCGCTGGGCGCCCGCGGCATCGCGCTGCTGGGGCTCTACTTCGGGTGCAGCCTGAGC GCCCCCTGCGCGCAGCTCAGCGCCAGCCTGGCCGCCTTCTACGGTCGCCTGCGAGGGGACGCGGCGGCCG GGCCGGGGGCCGGGGCGGGGGCCGGCGCAGCCGCGGAGCCAGAGCCCCGGCACCGCCTGGAGATCGTCTT CGTGTCTTCGGACCAAGACCAGCGGCAGTGGCAGGACTTCGTGCGGGACATGCCATGGCTGGCGCTGCCC TACAAGGAGAAGCACCGGAAGCTTAAACTTTGGAACAAATACCGAGTCTCCAACATTCCATCGCTAATAT TCCTAGACGCCACCACAGGGAAGGTTGTGTGCAGGAATGGGCTCCTGGTGATCCGAGATGACCCGGAAGG TCTGGAATTCCCTTGGGGACCTAAACCCTTCCGGGAAGTTATTGCGGGGCCCTTGCTTAGAAATAACGGG CAGTCGCTGGAGAGCAGCAGCCTGGAGGGGTCTCACGTGGGAGTCTATTTCTCTGCACACTGGTGTCCAC CCTGCCGAAGCCTTACCAGAGTGCTGGTGGAATCCTACCGGAAGATCAAGGAGGCAGGCCAGGAGTTTGA GATCATCTTCGTTAGTGCAGACAGGTCAGAGGAGTCTTTCAAACAGTACTTTAGTGAGATGCCATGGCTC GCTGTCCCCTACACTGATGAGGCCCGGCGGTCTCGTCTCAACCGACTGTATGGAATCCAAGGCATCCCCA CGCTCATCGTGCTGGACCCGCAGGGGGAGGTGATCACTCGGCAGGGTCGGGTGGAGGTGCTCAATGACGA GGACTGCAGGGAGTTCCCGTGGCACCCGAAGCCCGTGCTGGAGCTCTCTGACTCCAACGCTGTGCAGCTC AACGAGGGCCCCTGCCTCGTCCTTTTTGTAGACTCTGAGGACGACGGAGAGTCCGAGGCAGCCAAACAGC TGATCCAGCCAATAGCTGAGAAGATCATTGCCAAGTACAAAGCCAAAGAAGAGGAGGCGCCGCTGTTGTT CTTTGTGGCCGGGGAGGATGACATGACTGACTCCCTGAGAGATTACACCAACCTGCCTGAGGCGGCGCCT CTGCTCACCATTCTGGACATGTCAGCCCGGGCCAAGTACGTGATGGATGTAGAAGAGATCACCCCAGCCA TTGTGGAGACTTTTGTGAATGACTTCCTAGCAGAAAAACTCAAGCCTGAGCCCATCTAGTGGGCTCCGGC CTCTGGAGACAGTATTTAAGACACAGTCTTCTCCCCTTCCTCCCCTCTGCCCTTGGACTTTTCCAGCGTG TCCTGAATCACACAATCCAAGTGTCCAACCTCTGTGGTGCCTTGTTTCTACATTAGCAACTCAGCTAGCA CCCATGGGTGTGCATCCTCACAGTGGCAGCAGGTCCCACGGTGTTTGGAGATGGCTTGGGGATCCGTGAG GATGGCATGACCAGCTAGCCCCGAGACTGCCACCTCTCCTGGCTCATGATGGAGGACCTTCCCGGGTCTT CTGCAGAGTGAGCAAGCAAGGACCGTGGATTGAGGCCCTGACTCTGACTCTGGGATCAGTATACACACCC CAAGCCAGAGGGAACTCTCCTGTGACATCTGTACCCGTGGGAGTTACAGAGGAAGGGTCATGACCTCAGT GGCCATCAGCTGAGAGCTGTCGGCGAATTTGCTTTGTAAGGACGTGATCTTTCTTGGTCTGAGGGAAAAC CAGCGCATCAGTGGGTTCTTGGGCAGGGGACTTCCATATCTCTAAGGAACCTTGTGGGTGTTTGTTTTGG TGGGAAAATGAATAGCCGAGCTAACAGTTCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312910 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312910 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17392 same embryo
	EMAGE:17391 same embryo
	EMAGE:17390 same embryo
	EurExpress:euxassay_003834 same experiment
	MGI:4826839 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source