EMAGE mouse gene expression spatial database

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EMAGE:17400

Rfk riboflavin kinase ( MGI:1914688)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003837. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003837_01	euxassay_003837_02	euxassay_003837_03	euxassay_003837_04

euxassay_003837_05	euxassay_003837_06	euxassay_003837_07	euxassay_003837_08	euxassay_003837_09

euxassay_003837_10	euxassay_003837_11	euxassay_003837_12	euxassay_003837_13	euxassay_003837_14

euxassay_003837_15	euxassay_003837_16	euxassay_003837_17	euxassay_003837_18	euxassay_003837_19

euxassay_003837_20	euxassay_003837_21	euxassay_003837_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17400_wholemount_strong.wlz

17400_wholemount_moderate.wlz

17400_wholemount_weak.wlz

17400_wholemount_possible.wlz

17400_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17400_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7795
Entity Detected:	Rfk, riboflavin kinase ( MGI:1914688)
Sequence:	sense strand is shown >T7795 CTGGGATCTGACGGCTGGGTGCTATCCTGCGGGCAGGCACTGGTCGCGGCAGGTGTCTCGCTCCGCCGGC CCAGCTCCCGCGGGATCCCCCGGCTGGTCCGGGCCCCGGCGCCGCACTCACGGCGGCTCCGTCGCAGGCA CGGAGCGAGGAGCAGCCCGCGTCCTCCGAGGTCAGCGTCATGAGGAGCCTGCCGTTCTTCTGCCGCGGCC AGGTGGTGCGTGGCTTCGGCCGCGGCTCCAAGCAACTGGGCATCCCCACAGCCAATTTTCCTGAACAAGT GGTGGACAATCTTCCAGCTGATGTGTCTACTGGCATTTATTATGGTTGGGCCAGCGTTGGGAGCGGCGAT GTCCATAAAATGGTGGTGAGCATTGGATGGAACCCGTACTATAAGAATGTGAAAAAGTCCATGGAAACAC ACATCATCCATACCTTCAAAGAGGACTTCTATGGGGAAATTCTCAATGTGGCCATTGTTGGCTATCTCAG ACCTGAAAAGAACTTTGATTCTTTAGAGTCACTTATTTCTGCAATTCAAGGTGATATTGAAGAAGCTAAA AAGCAACTGGATTTACCCGAACATTTGAAACTCAAAGATGATAATTTCTTCCAAGTTTCTAAAGGCAAAA TTATGAATGGCCACTAATGAAAAGTCCCTTACCTGCCTACCACTAGTGTCACGCATAGTGTCACAGTGCT TCTGATACACTCTCATCTTGATCATATTTTAAATCAAGCGTTTTCCTGTAGCTGTCAAGTGGTCTCCAGC AGTTCACTCCATCATGTTGCACAGTACTAAGAGACTCATTCAGAAATCAAGACTTTAGATGTAATATGTT GATTAAAATGTAGCACTAGAAAGCTTGTACTGAAAATAAAGTCTGTGAGTGTGGGTGGAAAAGCAAGTTC CTGAGGAAAATGAGGACTGACCATAGTTCTTGTGACTGAGTGTGCTGGCACTGGTGAGCCCAGCCAGCCT GTGCTGAACAGAGGCTTTTCAGGAAGTGCACCACACACACACACACACACACACACACACACAGAGCTTA CCCATGAAGGAAAAATTAGAGTTACTTTTCTCATTTTGTGCATTGATATATTTTTACAATCCTGCAAACT TCCTTGAGTCACTCACGACTCCCCCTGAGGTCACTTCCTGGTAGTGTCCCAATGCTCTGTGTGCAGTTAC TTCAGGTTGAAGTTGCCTTGATCGCCTGCCCCTCATCAGGGCTTTTGCTTTTCTTCAAAGTACTGAGGAT CCAACCCAGTGCCTTACACGCCAGGCAAGTGTGACCACTGAGCTAGGCTCCCGCCCCCTTTACTTTTTGA AGCCATACAATGGATGTCTATATGTTGCCTTTTTGAAGATTAGGTGGTTTTGCTCTTTAACATTTTTGTG ACTTTGGTTTATTTTCTTCCCATGTCGCCCAGAGATTACTTGTGTGTGTGCCATTCTGCTCAAAACTAAA TGGTGGTCAGGTTCAAGTAAAGAAAAATAAAACAAGCTGCATTTATAGACTTGCTTGGAATTGAAATGAA CATAGCCAGTTCCTACCCAACCCTGCCCTGCCTTTTTTTTTTTTTTTAAAGTGTGGTCCTGTGCTTTCAA AGTAAGTACAGTTCTCTATGACCAGGCACTTAAACATAATTTAAAAGTCCTTGTAGCTTCAGAACCGGAT AAGTTCCAGTTGCATATCTCTATATCTCCTGGTTTCCCTCTTGGTTTTCCCCTGGTTCCTCCCCTTTCCC TCTTGGTGGCCTGAGTTGTTTATGTTGGCAGAAGGTTGACATTGGATGCTCGATTGCTTCAGCCTCACCG GGGCATTGAATCATCATTTGTTGAATGGCAACAGGTTTGCTGGGGCAGAGGCACTGCCTAAGTGGGAGCA TGCACGTGAAAATAGAAAGGTAATTTAACAACACACAGACACCCAGAGACTTGGTCTCTGCATGTGTGTA GAAGTAGTGGTGTGGGGAGGGATGGGACTCACTCAGCCCTGTGAATGTGTTCTATGGCTGAGCAACATCC CTAGCCTTCAGAATAGAATTTTTGATGTTGGAAATCCTGTATTTAAAGGAAACCTTAATTATCAATGGAT TTATGAAGGAAAGTTAAAAACAAATATGCACAGTACAAAATAGATCTGTGGCTTTTTTTTTTTAAAGTAA GAATAGCTTGTTAGTATATCAATGTTCATGGCTAATGTTATCTCATTTTGTTAGTCGTTATTTTGGAATT ACATGATGTGTGATTTAGGGCTTAGTTAATTCTCTAGAATTCAAAAACTATTATTGATGTATTAAAAGAA ATTACGGTACCACGTTGATTAGTCTGAATGTTTGCAGGCATTGGAATAAAATTTGACTTATTTTTTCCCT CTTCCCCTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6315425 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6315425 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17404 same embryo
	EMAGE:17401 same embryo
	EMAGE:17403 same embryo
	EMAGE:17402 same embryo
	EurExpress:euxassay_003837 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source