EMAGE mouse gene expression spatial database

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EMAGE:17404

Tmem184b transmembrane protein 184b ( MGI:2445179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003828. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003828_01	euxassay_003828_02	euxassay_003828_03	euxassay_003828_04

euxassay_003828_05	euxassay_003828_06	euxassay_003828_07	euxassay_003828_08	euxassay_003828_09

euxassay_003828_10	euxassay_003828_11	euxassay_003828_12	euxassay_003828_13	euxassay_003828_14

euxassay_003828_15	euxassay_003828_16	euxassay_003828_17	euxassay_003828_18	euxassay_003828_19

euxassay_003828_20	euxassay_003828_21	euxassay_003828_22	euxassay_003828_23	euxassay_003828_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17404_wholemount_strong.wlz

17404_wholemount_moderate.wlz

17404_wholemount_weak.wlz

17404_wholemount_possible.wlz

17404_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17404_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7788
Entity Detected:	Tmem184b, transmembrane protein 184b ( MGI:2445179)
Sequence:	sense strand is shown >T7788 CCGAACGGGGCGGGGAGACGTCGGTCCTCAGTGCAGTGGCTTTGACCTGGCCCGGTGGTCGGCCCTTGAG ACGGCCAGCCCGTCACTCACGGCACGAGATCCTCTCAGGGAGCCTGCTGCTGCTGGGAACCATGACAGTG AGGGGGGCCGCGCTGGCCCCAGACCCAGCGTCACCCACAACCACAACAGCTTCACCTAGCGTCTCTGCCA CCCCCGAGGGCAGCCCCACAGCCATGGAGCACCCTGTGTTCCTGATGACCACTGCCGCCCAGGCCATCTC CGGCTTCTTTGTGTGGACAGCTCTGCTCATCACTTGCCACCAGATCTACATGCATCTGCGCTGCTACAGC CGTCCCAATGAGCAGCGCCACATCGTGCGCATCCTCTTCATCGTGCCCATCTACGCCTTCGACTCCTGGC TCAGCCTCCTCTTCTTCACCAATGACCAGTACTACGTGTACTTCGGCACCGTCCGAGACTGCTATGAGGC CTTTGTCATCTATAACTTCCTGAGCTTGTGCTATGAGTACCTCGGGGGAGAGAGTGCCATCATGTCTGAG ATCAGAGGGAAGGCCATTGAGTCCAGCTGTATGTACGGCACGTGCTGCCTCTGGGGAAAGACCTACTCCA TCGGATTCCTGCGGTTCTGTAAACAGGCCACCCTGCAGTTCTGTGTGGTGAAGCCACTCATGGCCGTCAG CACCGTTATACTCCAGGCCTTTGGCAAGTACCGGGACGGAGACTTTGATGTCACCAGTGGGTACCTCTAC GTGACCATCATCTACAACATCTCCGTCAGCCTGGCCCTATATGCGCTCTTCCTCTTCTACTTTGCCACAA GGGAGCTGCTCAGCCCCTACAGCCCTGTCCTCAAGTTCTTCATGGTCAAGTCCGTCATATTCCTCTCCTT CTGGCAAGGCATGCTGCTGGCCATCTTAGAGAAGTGCGGGGCCATCCCCAAGATCAACTCAGCGCGAGTG TCAGTGGGTGAGGGCACCGTGGCTGCCGGCTACCAGGACTTCATCATCTGTGTGGAGATGTTCTTCGCGG CCTTGGCCCTGCGGCATGCCTTCACCTACAAGGTCTACGCTGACAAGAGACTGGACGCTCAAGGCCGCTG CGCCCCCATGAAGAGCATCTCCAGCAGCCTCAAAGAGACCATGAACCCACACGATATTGTGCAGGACGCC ATCCACAACTTCTCGCCAGCCTACCAGCAGTACACGCAGCAGTCCACTCTGGAGCCCGGGCCCACCTGGC GTGGCGGCACGCACAGCCTTTCCCGCTCCCACAGCCTTAGTGGTGCACGCGACAATGAGAAGACTCTGCT GCTCAGCTCTGATGATGAGTTCTAAGTGTGGGCCCTGGAGGGGAAGGAGAGGTGCTTCCCCCAGCCCCCA GCCAGGCTGGGGAGCAGAGCAACCAGCACAGCATGGCTGCTGCCCTTTTATTTATTGGACCAGAAACTCG CATATCACTTCCAGAGGAACAGGCCTCAGGAATATTTCTCCCTGGCCAGCCCTGCACTAGCTGGGGCAGG GGCAGAGGACAGCGGCTGTAGCTGTCACATCTCTGCGACTCATCCTGTACCGTGTGTTGGCCAGAAGCTG CATCCTAAGCCCCCTCCCCAACAAGATATCTGCCGAGCTGTCAGGCCCAGCCCACCCTACACCGTGCAAT ACCCAGACCCAGCCTGGTCTTTCCCCAGGCCTTTGCCCGCTTCTCCCTGGTCACCCTTGTCCAGTGTGAG TTTTGTCACATTCTGGGCAGGTAGAGACGTGAATGCTCTGTGGACCCTCCCCCTCTCCCAACCTAGGCCT GTCTGGCATGCTGCAAGGACCAGAGCCAGCCCCCAGGAGCCTCAGGGCCCACTTAGGAAGGATGCCTTGT GCCTATGGGCCCTTTGGTGACATGTGGGGCTCTGGATCCTTGGGTGTCCTCACTGTGGCCACTCTGCCTG CTGAGGACAGCCAGAAGCCCACACTGCCCTCTCACTTGGGTCCAGTTCTTGATAGTTGGGGGCAGAGCCC CCACACTCCCTTATACTTCCTGACTGTGCCAGGGCTTGGGTTCCCCACCACCTCCTCAATGGAGCAAAGA CTCCTGCTAGGCCCTGGTCTCCCTGGGGTTTATCCCTCCTGGGTCATGGGAAAAGTCAGTCTCCACATTA CAGATCACTTCCAGAGGGAGGCTGGAGGGATGGTCATACAGCGGGGCCAAGGTAGGGGTGGTCTCAGACC CTCCTGGGTCCCCTGACCATACAGGGCCTGGTGGTCTGCTCCATCTGGCCCCTGGCCCATCTCTTCTGTG CCTTAGTCACATATGAAAGCCCCTCCCCAGTTCCCGTCTGCTCCAGGCACTCCTCGTTGAGCTGCTGGCA CTCAGGGTTCCGCAGCGCTGGGTTGGGCATCCTTGGGACCACCCACCCTCTCCCTGGCTCCCTCTACACG CACATCCCAGAGCTGCCTCCCAGGGCTGGGATCTCAGAGGTAGGGTGGCAGGGGCCTGGTAAGCAGAATT CAAGCAGCACTTGGTCTTGGCTCCAGTAGCCTCTTGAGGTGGAGAGTGGTGGGGAGCCCTACCTTGTAGC CTTCAGTGGATGCTCTCCCCTTGGTCACCTCTGGAACAAACAGGTTCCTTCCCTGGGGTCCATAGGGAGG GACCTCATGGGGCTGTCACATCCTAGCAACCTTCTTAGCCTCCTTCCTTCCTTCCACACGGCCCAGAGGC CAGCTCGGCCCCACCCAGAGGGCCTGGCCAGCTGCTCCCAAGCCACCCAGGGAGGCAGCAGACAGGTGGG AGCTCCTGGGGCACGGCCGTGGGATTGCTTCCACCAGTGTGTTTCTGAGACTTCTCCTTATCTTCCAGGC CAAAGTATGTTGCTGCTTCCTGTCTCCACTGGTCCCCACCTACCCTGGCACTTGCCCTGTGACATGATGG GTGAGGCTGTGGGGGAACTCAGTGGCAGGAAGCTAGTGGTCCCTGGGAACAGGGGACAGGAATCCGTACC CAACTGCCCCGTTCTTTCTTCCCATTCTTTCTTCCTAGGATGCATAAACTATCTTGTCTCATTTATTTTT TAATTTTTTTTCAGATAGAGTAGCTCTTTGTACATAAAAAAAATACTTGAAAATTGTATGATGTATGAGA AGACAGAGCCCCCTAGTTTTGTATTTTGTCCTGTGACTGCCATGCGTTCCACCAGAAAGCCGCTCTATTT TGGTCTCTGTGATATTTTAAATGCGTGACAGAAGCAAGCAAATAAAGTCAGGAAGAAATATATATATGAG ATAATATAGATTGTATTGAAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6314456 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314456 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17401 same embryo
	EMAGE:17403 same embryo
	EMAGE:17402 same embryo
	EMAGE:17400 same embryo
	EurExpress:euxassay_003828 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source