EMAGE mouse gene expression spatial database

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EMAGE:17422

Bpifa1 BPI fold containing family A, member 1 ( MGI:1338036)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003858. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003858_01	euxassay_003858_02	euxassay_003858_03	euxassay_003858_04

euxassay_003858_05	euxassay_003858_06	euxassay_003858_07	euxassay_003858_08	euxassay_003858_09

euxassay_003858_10	euxassay_003858_11	euxassay_003858_12	euxassay_003858_13	euxassay_003858_14

euxassay_003858_15	euxassay_003858_16	euxassay_003858_17	euxassay_003858_18	euxassay_003858_19

euxassay_003858_20	euxassay_003858_21	euxassay_003858_22	euxassay_003858_23	euxassay_003858_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17422_wholemount_strong.wlz

17422_wholemount_moderate.wlz

17422_wholemount_weak.wlz

17422_wholemount_possible.wlz

17422_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17422_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7787
Entity Detected:	Bpifa1, BPI fold containing family A, member 1 ( MGI:1338036)
Sequence:	sense strand is shown >T7787 GGACGACCACTGAGACCTTGAGACTCAGACACCAAGAGAGATGTTTCTAGTTGGGAGCCTCGTTGTCCTC TGTGGGCTGCTGGCCCACAGCACAGCACAGCTGGCAGGCTTGCCATTGCCCCTGGGCCAGGGTCCACCCT TGCCACTGAACCAGGGCCCACCGTTGCCACTGAACCAGGGCCAGCTGTTGCCCCTGGCTCAGGGTCTGCC TTTGGCTGTAAGCCCAGCACTGCCTTCAAATCCCACAGATCTTCTTGCTGGAAAATTCACAGATGCTCTC AGCGGTGGCCTGCTGTCTGGGGGGCTGCTGGGCATTTTGGAAAATATTCCACTCCTGGATGTTATAAAGT CTGGAGGAGGCAATTCTAATGGCCTTGTTGGGGGCCTGCTGGGAAAACTGACGTCATCAGTTCCTCTCCT GAACAACATCCTCGACATAAAAATCACTGATCCGCAGCTGCTAGAACTTGGTCTTGTGCAGAGTCCTGAT GGCCATCGTCTCTATGTCACCATCCCTCTGGGCTTGACACTCAACGTAAATATGCCCGTAGTTGGAAGTC TTTTGCAATTGGCTGTGAAGCTGAACATTACTGCAGAAGTCTTAGCCGTGAAAGACAATCAGGGGAGGAT TCATCTGGTTCTTGGTGACTGCACCCACTCCCCTGGCAGCCTGAAAATCAGCTTGCTCAATGGAGTCACT CCTGTTCAAAGCTTTTTAGACAACCTCACAGGGATATTGACTAAAGTCCTTCCTGAGCTGATCCAGGGCA AGGTATGTCCTCTGGTCAATGGGATTCTCAGCGGTTTGGATGTCACCCTGGTGCACAACATTGCTGAATT ACTGATCCATGGACTACAGTTTGTCATCAAAGTTTAGGCATCCCAGGAAGGAAGGCTATCTTGGCTGAGC TGAATCATTTCTTGCTGCTCAGTCTCCTGCCTCTTGCCCAGTCTCCCATGGCTCACAGAAAGGGGCCCAC ATCCTGGAAAATTATGTCTTCCTTCTCCTCACGGAGCCTGATCTCTTCCCATCAGGCACGATTAATCCTG TGATCCTCACTAAATAAAATAGCTCTTCATCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:6314015 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314015 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17424 same embryo
	EMAGE:17423 same embryo
	EMAGE:17427 same embryo
	EMAGE:17426 same embryo
	EMAGE:17425 same embryo
	EurExpress:euxassay_003858 same experiment
	MGI:4827288 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source