EMAGE mouse gene expression spatial database

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EMAGE:17525

Cct8l1 chaperonin containing TCP1, subunit 8 (theta)-like 1 ( MGI:2685289)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005847. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005847_01	euxassay_005847_02	euxassay_005847_03	euxassay_005847_04

euxassay_005847_05	euxassay_005847_06	euxassay_005847_07	euxassay_005847_08	euxassay_005847_09

euxassay_005847_10	euxassay_005847_11	euxassay_005847_12	euxassay_005847_13	euxassay_005847_14

euxassay_005847_15	euxassay_005847_16	euxassay_005847_17	euxassay_005847_18	euxassay_005847_19

euxassay_005847_20	euxassay_005847_21	euxassay_005847_22	euxassay_005847_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17525_wholemount_strong.wlz

17525_wholemount_moderate.wlz

17525_wholemount_weak.wlz

17525_wholemount_possible.wlz

17525_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17525_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9771
Entity Detected:	Cct8l1, chaperonin containing TCP1, subunit 8 (theta)-like 1 ( MGI:2685289)
Sequence:	sense strand is shown >T9771 CGCAAAGTGGAGGCCCACTTCCGGTTGGCTTCTGCAGGTAGCAAGTGATGTGCATTTGACTTCTGGAGCA GCATCAAAGACAGATGCCAAGGTGTGCCTACTGCCATTTCCAGAGTGCTTCTAACGGTTAGTCTAACCCA TGGCGGTCTCCCCACCCTCCCGGGCCACACAAACCAAAGTTCAATCAGACCTAGAACTTCCCCAGCGGCT GAAGCCAGGCCTAGAGAAAACCCCTGAATCTCAAGGAGAAGAGCCATCATACATACTGAGGGCCACAGCT GCAGCTCAGACCCTAGCCAGTATCATCCGGTCTTGTTATGGTCCCTTTGGCCGGCAGAAGTTTCTGGTGA CTGCCAAGGGAGAAACAGTTTGTACAGGTCATGCCGCTGCCATCCTCAAGGCTCTGGACCTGGAGCACCC AGCTGCCCAGTTTGTTCAAGAATTAGCCCAAACCCAGGTAGAGAACGCTGGGGATGGCACGGTCTTTGTT GTTCTACTGACAGAAGCCTTGCTGGAGCAGGCCCATTACCTTCTGTGGGCTGGCCTAACTCCAACCCAGC TCCGGGAGGCCTTTGCCACAGCCACAGCAGAGGTTCTGACCGCTCTGCCTTCCCTGGCCATCCGCTCTCT AGGGCCTTTGGAAGATCCTTCCTGGGCCCTCTATTCTGTGATAAGTACTCACACCCTGTCCAACTCAGAT TATTTAACCAAGCTTGTAGCTCAAGCGTGCTGGGTTAGCAGGGAGCCAAATGGCAGCTTCAAGCCTGAGA GTATTGTCGTGTGCGTACTTCAGGGTGGGAAACTGACTGATTCCCGAATTTTTCCTGGAGTAGCAATAGC GGGGAAGCTGTGTGGACAAAAGACTGAGGTCCTTGGTGATGCTAGGGTGGCCCTGTTCAATTGCCCCTTT GGTCCTACAAATCCATTTACACTGGCCACACCACGTCTCTCCAACCCGGAAGAACTACTAAGATTTCGGA AACAAACTGAACAAGTGGAAAAGGAAATAGCCCAACTAGCCATTATGGACATTAACGTGGCAGTGGTCTT GGGGGAAGTTAATGAGAAATCGGTGGACCAGGCTAACTACTGTGGCATCATGGTGATTCAAGCCAAGTCC CGAAAGGAGATTGTCTACCTGAGTGAGAAGCTGGGCACTCCTCTGCTCGGCCGGGTCCTTCCTCCCCTGG AGCCAGGGAAGTGCCACAAGGTTTACAGGAAGGAATTTGGAGATACTGCGGTGGTAATGTTTGAATGGGA ACATGAGATTGCACCTTTTCTCTCAGTGGTCCTTAGGGGACCCACCATCCAGGGACTTCGGGTTGCAGAG CAGGCTGTCTACTATGGCATTGATGCATTTTCTCAGCTGTGTCAAGATCCCAGACTACTGCCAGGAGCAG GTGCCACAGAGATGGCTCTGGCAAAAATGCTGGTAGACAAAGGAAGCCGACTGTCTGGCCCCAACGGTCT GGCCTTCCAAGCATTTGCTCAAGCCCTGAGTTCTCTGCCTAAAACCCTGGCAGAGAATGCAGGCTTAGCT GCCCAAAGCGTGATGGCAGAATTGAGTGGATTTCACCAAGCTGGGAACTTCTTCGTTGGAGTGGGAACAG ACGGCTTAGTAAATGTGACTCATGAAGGTATATGGGACATACTGAGGACCAAAGCCCAAGGGTTACAAGC AGTTGCTGAGCTGGTGCAGCAGCTGGTGACTGTGGACCAAATTATAGTGGCCAGAAAGACTCCTTTGTAC AGACAGATCACAGACCCCACCTTGAATGCAAAGGTATCCTCACCCCTGAGAGCAAAATTCTTTGGAAAAT ATGTATAGCGGCACACTAAATAAAGAATGGTCAGGATTGCAAAGTGACCTCCAAAATAAAAAAAGAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6743432 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6743432 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17526 same embryo
	EMAGE:17527 same embryo
	EMAGE:17528 same embryo
	EMAGE:17523 same embryo
	EMAGE:17524 same embryo
	EurExpress:euxassay_005847 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source