EMAGE mouse gene expression spatial database

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EMAGE:17542

Il7r interleukin 7 receptor ( MGI:96562)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005872. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005872_01	euxassay_005872_02	euxassay_005872_03	euxassay_005872_04

euxassay_005872_05	euxassay_005872_06	euxassay_005872_07	euxassay_005872_08	euxassay_005872_09

euxassay_005872_10	euxassay_005872_11	euxassay_005872_12	euxassay_005872_13	euxassay_005872_14

euxassay_005872_15	euxassay_005872_16	euxassay_005872_17	euxassay_005872_18	euxassay_005872_19

euxassay_005872_20	euxassay_005872_21	euxassay_005872_22	euxassay_005872_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17542_wholemount_strong.wlz

17542_wholemount_moderate.wlz

17542_wholemount_weak.wlz

17542_wholemount_possible.wlz

17542_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17542_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 06 07 08 14 15 16

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10020
Entity Detected:	Il7r, interleukin 7 receptor ( MGI:96562)
Sequence:	sense strand is shown >T10020 ATTCACTTGCACATACAAGCGTGCTTCTTCTCTATTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT CTCTCTCTCTCTCAGAATGATGGCTCTGGGTAGAGCTTTCGCTATAGTTTTCTGCTTAATTCAAGCTGTT TCTGGAGAAAGTGGAAATGCCCAGGATGGAGACCTAGAAGATGCAGACGCGGACGATCACTCCTTCTGGT GCCACAGCCAGTTGGAAGTGGATGGAAGTCAACATTTATTGACTTGTGCTTTTAATGACTCAGACATCAA CACAGCTAATCTGGAATTTCAAATATGTGGGGCTCTTTTACGAGTGAAATGCCTAACTCTTAACAAGCTG CAAGATATATATTTTATAAAGACATCAGAATTCTTACTGATTGGTAGCAGCAATATATGTGTGAAGCTTG GACAAAAGAATTTAACTTGCAAAAATATGGCTATAAACACAATAGTTAAAGCCGAGGCTCCCTCTGACCT GAAAGTCGTTTATCGCAAAGAAGCAAATGATTTTTTGGTGACATTTAATGCACCTCACTTGAAAAAGAAA TATTTAAAAAAAGTAAAGCATGATGTGGCCTACCGCCCAGCAAGGGGTGAAAGCAACTGGACGCATGTAT CTTTATTCCACACAAGAACAACAATCCCACAGAGAAAACTACGACCAAAAGCAATGTATGAAATCAAAGT CCGATCCATTCCCCATAACGATTACTTCAAAGGCTTCTGGAGCGAGTGGAGTCCAAGTTCTACCTTCGAA ACTCCAGAACCCAAGAATCAAGGAGGATGGGATCCTGTCTTGCCAAGTGTCACCATTCTGAGTTTGTTCT CTGCGTTTTTGTTGGTCATCTTAGCCCATGTGCTATGGAAAAAAAGGATTAAACCTGTCGTATGGCCTAG TCTCCCCGATCATAAGAAAACTCTGGAACAACTATAGTCTGAATGTGAGTTTCAATCCCGAAAGTTTCCT GGACTGCCAGATTCATGAGGTGAAAGGCGTTGAAGCCAGGGACGAGGTGGAAAGTTTTCTGCCCAATGAT CTTCCTGCACAGCCAGAGGAGTTGGAGACACAGGGACACAGAGCCGCTGTACACAGTGCAAACCGCTCGC CTGAGACTTCAGTCAGCCCACCAGAAACAGTTAGAAGAGAGTCACCCTTAAGATGCCTGGCTAGAAATCT GAGTACCTGCAATGCCCCTCCACTCCTTTCCTCTAGGTCCCCTGACTACAGAGATGGTGACAGAAATAGG CCTCCTGTGTATCAAGACTTGCTGCCAAACTCTGGAAACACAAATGTCCCTGTCCCTGTCCCTCAACCAT TGCCTTTCCAGTCGGGAATCCTGATACCAGTTTCTCAGAGACAGCCCATCTCCACTTCCTCAGTACTGAA TCAAGAAGAAGCGTATGTCGCCATGTCTAGTTTTTACCAAAACAAATGAATTATAAGAAAACCCTTCCAT CGACAACCAAATGATCACTGAGATGGAAGGTCTGGAATGCTTGCTCTCCCCCGTAGCTCACAGAAGAGAA AGTCAACGTGACCTTGCTACACATCTTCAGCATTCTAAGAAATCATTTTGCTCTTCTAGCTCAGAAGCAT TTGCACAAAGCAGGAAGAATCTGTTTTCCCTGTTGTTGGATTAGTCATAAGAGTCCATATGACCCAATTA AAATTGCAAAACTCAGTTAAGTGAAGAAAGAAAGATAGACAAAAGAAGATAGAAGGATGTGGTGAATGCA GGAAGAAGAAAATGAAAGATGTGAGTGGTGGGTCTATCATTCAAATTGACTATTTATCCAGCACTATACC ACTCTTCTCATTTCTTCCTCACAATAATATTACAATGTGGGCTTATCCATTATAACTTTTATTTTCTTTG TCATAGATGCTGAAGTTGAAAGTAGAGATTTTAAGTGATATCCAAATTTTTCTTTCAGCTACAGATGAGG CACACATTCCAACTTCAACCCTCTCTTGCCATGAACCTGTCCTATTGTTGAGTGTCAAACATCACCACTA AGTGGATGGTTATGTAGTCCATTATCCAAACTGAGTCGTTTTGGAAAGAAAAAGTTAGACATAATTAACA GTAAGCATAAACTGTATATGTCTAAGAGAGATGTGGATGGATGGTCATTTTACTTAAAGTGGCTATAGGG ATGAACATGAAGGACAAAGTACATTTATGGGTGTGGCATACCATGACCATGTGTCAAAGGAAGTGGGAAA AAGAAAAAAAAAGCACCAAGATCATTTGATTTTGTTTTGTTGTTTTGTTTGAAAACAAACTCAAGAAGCA ATGAGTTAGAAGCCGAGAAGTTCCAGAGTCAGTTATCAAGACCATGATTTTCCTGCTGCTATTATCCATT GGCTTCTCTGTGACATTGTAGGAGGAACTATGGCCAATCTACAGGAGTTCAACATTTAACAGTGAATGGA GTCCTCCTATGTGAGTCCTCCTATGTGTGGAGACACCATTAAGAACTACCCCAAGTTCTACATCTCTGGA TATTGCCTGAACTACAGAAAAAGGGGGCTGCGCACACCACAATGAGTGCCCTACCTGAAACTATGCTCAC AGAAACACAAAGAAGATGGGTAAGTTATTCAAATTCAAATGTTGATTTATGACTGCAAGTCACAATTTTG AATCCCTGCTGTGTATAACCAATCTCCTGAAGAAAACAACAAATAACTGAAAGATACTGTGGTTGGGTGC CTTAGCATTAAAATTCTGTTTAAGTGTTGACATTGTTTATTTGGATTGGAGTGTCTGTCCGGTCATGTAT TGTATCCATGCATTATATTCAGATAACCACAACAGCTGCTAATGCTTGATTATATTCTCAGGGACTGCAT GCAATGTAACATTACTGGTTGGTTCTGCCAATTTTCCTCTTGGTATTTATAAAGGAAAACCAAAACTCTT GGTCAGAGACAATATGCAAAACAGAGATGTCAAGTACTATGTCCAAATACTGTGAAATATAATGAGAAAT AGGTAACAAATTTATCAATCAACTATGTTTGGATCCAGGGAATCTCAAGTTATTCAATTCATTCTCTGTA AGCCTTTGTCTCTCTCTTCATCCAGACTTTTGCCTTCAAATACAAGCATGCGCTATTTTCTGGAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6758487 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6758487 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17546 same embryo
	EMAGE:17545 same embryo
	EMAGE:17544 same embryo
	EMAGE:17543 same embryo
	EMAGE:17541 same embryo
	EurExpress:euxassay_005872 same experiment
	MGI:4825583 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source