EMAGE mouse gene expression spatial database

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EMAGE:17545

Slc43a3 solute carrier family 43, member 3 ( MGI:1931054)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005875. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005875_01	euxassay_005875_02	euxassay_005875_03	euxassay_005875_04

euxassay_005875_05	euxassay_005875_06	euxassay_005875_07	euxassay_005875_08	euxassay_005875_09

euxassay_005875_10	euxassay_005875_11	euxassay_005875_12	euxassay_005875_13	euxassay_005875_14

euxassay_005875_15	euxassay_005875_16	euxassay_005875_17	euxassay_005875_18	euxassay_005875_19

euxassay_005875_20	euxassay_005875_21	euxassay_005875_22	euxassay_005875_23	euxassay_005875_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17545_wholemount_strong.wlz

17545_wholemount_moderate.wlz

17545_wholemount_weak.wlz

17545_wholemount_possible.wlz

17545_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17545_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10021
Entity Detected:	Slc43a3, solute carrier family 43, member 3 ( MGI:1931054)
Sequence:	sense strand is shown >T10021 CACATCACTACCAGTGGTCCTCTCTGAAGCTCAGGACAGGATTTGTGGACTCACTTCACCTCAGACCATG GCAAGCAAGGGCTTGCCCCTTTACTTGGCCACCTTGTTGACTGGACTCTTGGAATGCATCGGTTTTGCTG GTGTCCTCTTTGGCTGGACTTCACTGTTGTTTGTGTTCAAAGCAGAAAACTACTTTTCAGAGCCCTGTGA ACAGGACTGCTTGCTCCAGAGCAATGTAACAGGGCCTTCTGATTTAAAAGCGCAGGATGAGAAGTTCTCA CTCATCTTTACCCTGGCATCCTTCATGAGTAACTTCATGACCTTTCCCACTGGCTACATCTTTGACCGCT TCAAGACTACTGTGGCCCGCCTGATAGCCATATTTTTCTACACCTGCGCCACGATCATCATTGCCTTCAC CTCTGCAAACACTGCCATGCTGCTCTTCCTAGCCATGCCCATGCTCGCAGTGGGAGGAATCCTGTTCCTT ATCACCAACCTACAGATTGGGAACCTCTTTGGGAAACACCGTTCAACCATCATCACCCTCTACAATGGAG CATTTGACTCCTCCTCAGCAGTGTTCCTCGTCATTAAGCTGCTTTACGAGCAGGGCATCAGCCTCAGGTC TTCCTTCATCTTCATGTCTGTCTGCAGTGTCTGGCACATTGCGCGTACTTTCCTTCTGATGCCCAAGGGA CATATCCCCTACCCACTGCCTCCCAACTACAGCTATGGCTTGTGCTCCAGGTTTGGTGCTAGCAAGAAAG AGAATAAAGCAGCTGAACACGAAACCAAGGAGCTGCGGTCAAAGGAATGTCTGCCACCCAAGGAAGAGAA CTCTGGACCAGAACAGCAGCAGCAGCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGAGCAACACGAG CAACACTCTTTTCGACGCTGCGCGCTCTCTCGTCGATTCATCTTGCACGTGGTGTGGCTGTCTATCATAC AGTTGTGGCATTACCTCTTCATTGGTACTCTCAACTCTCTACTCACCAAGTTATCCGGTGGGGACAAAGT GGAAGTCAGTGCCTACACAAATGCCTTTGCCATCACCCAGTTCTTTGGCGTGCTGTGTGCCCCCTGGAAT GGCTTACTCATGGACCGCCTTAAACAGAAGCATCAGAAGGCAGCCAAAAGGACAGGTTCCTCGTCTGAGG CTGTGGCTCTCTGCTCCATGGTGCCTTCACTGGCTCTGACGTCTCTGCTGTCCCTGGGCTTTGCCCTGTG TGCCTCCATTCCTGTCATGCAGCTACAGTACGCCACCTTCATCCTGCAAGTGGTCAGCCGCTCCTTCCTG TATGGATGCAATGCTGCCTTCCTCACGCTGGCATTTCCCTCAGAGCATTTTGGAAAGCTCTTTGGGCTGG TGATGGCCTTGTCAGCTATTGTGTCTCTGCTACAGTTCCCCCTGTTCAAAGTCTCCCCTGAGAGCAACGC TGTGTATGTATCGATGGGGCTTGCCATTTTCCTGACATTGGTCCATCCCTTCCTGGTGTACCGTGAGTGC CGTGCTGAGAAGACAAAATCGTCTGTGGATGCCTAGTCCACTCTCTAGCCTCGATGATGCCTTTTCTGTT CATTACTCGACTTTGATGATCCAGTATCTACAAAGACTGCCTCCCCAGGAGAACTGTAATCGGCAGTCAT AGTTTTTCTTAAACCACTGCCACCACAAGAAACAAACCAACAAAAACCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6758493 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6758493 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17542 same embryo
	EMAGE:17546 same embryo
	EMAGE:17544 same embryo
	EMAGE:17543 same embryo
	EMAGE:17541 same embryo
	EurExpress:euxassay_005875 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source