EMAGE mouse gene expression spatial database

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EMAGE:17679

Shcbp1 Shc SH2-domain binding protein 1 ( MGI:1338802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006012. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006012_01	euxassay_006012_02	euxassay_006012_03	euxassay_006012_04

euxassay_006012_05	euxassay_006012_06	euxassay_006012_07	euxassay_006012_08	euxassay_006012_09

euxassay_006012_10	euxassay_006012_11	euxassay_006012_12	euxassay_006012_13	euxassay_006012_14

euxassay_006012_15	euxassay_006012_16	euxassay_006012_17	euxassay_006012_18	euxassay_006012_19

euxassay_006012_20	euxassay_006012_21	euxassay_006012_22	euxassay_006012_23	euxassay_006012_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17679_wholemount_strong.wlz

17679_wholemount_moderate.wlz

17679_wholemount_weak.wlz

17679_wholemount_possible.wlz

17679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 12 16 17 18

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T257
Entity Detected:	Shcbp1, Shc SH2-domain binding protein 1 ( MGI:1338802)
Sequence:	sense strand is shown >T257 GTAAATTTGAAATGGCTGATGATTTGCGGGCTGGTGGAGTTCTGGAACCTATAGCTATGGTTCCACCGAG ACCTGACTTGGCGGCGGAGAAGGAACCGGCGTCCTGGAAGGAAGGTTTATTCTTGGATGCAGATCCATGC AGTGATCAAGGCTATCATGCTAATCCAGGTGCTACTGTAAAAACTCTCATACCAGAAGGAAAAACTCCTT TTCCACGAATTATCCAAACAAATGAACTTCTGTTTTATGAACGATTCAGAGCCTATCAAGATTACATTTT AGCTGACTGTAAGGCCTCTGAGGTAAAGGAATTCACAGTCAGCTTCTTGGAAAAGGTCCTTGAACCATCT GGATGGTGGGCAGTCTGGCACACTAATGTGTTTGAGGTGTTGGTTGAGGTTACAAATGTGGACTTTCCAT CCCTGAAGGCGGTCGTAAGGCTTGCAGAGCCATGCATCTATGAATCTA
Notes:	The probe template was PCR amplified from IMAGE:2654132 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2654132 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17680 same embryo
	EMAGE:17677 same embryo
	EMAGE:17676 same embryo
	EMAGE:17678 same embryo
	EMAGE:17681 same embryo
	EurExpress:euxassay_006012 same experiment
	MGI:4828051 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source