EMAGE mouse gene expression spatial database

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EMAGE:17709

Zdhhc7 zinc finger, DHHC domain containing 7 ( MGI:2142662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006041. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006041_01	euxassay_006041_02	euxassay_006041_03	euxassay_006041_04

euxassay_006041_05	euxassay_006041_06	euxassay_006041_07	euxassay_006041_08	euxassay_006041_09

euxassay_006041_10	euxassay_006041_11	euxassay_006041_12	euxassay_006041_13	euxassay_006041_14

euxassay_006041_15	euxassay_006041_16	euxassay_006041_17	euxassay_006041_18	euxassay_006041_19

euxassay_006041_20	euxassay_006041_21	euxassay_006041_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17709_wholemount_strong.wlz

17709_wholemount_moderate.wlz

17709_wholemount_weak.wlz

17709_wholemount_possible.wlz

17709_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17709_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9566
Entity Detected:	Zdhhc7, zinc finger, DHHC domain containing 7 ( MGI:2142662)
Sequence:	sense strand is shown >T9566 CTGCTGCCTCTCCTCCAAGAACCGCCGAGTTCTTGGAAGCCCGGTCTTGGATGAAATCGCCAGAGCCTCC CCGCGATCCGCCTCCCGGAAGCCCCGGGCGGCGCGACCGGAAGGACGCAGGCGCAGCGTCTTGACCTCGG CCTGGTCCAGTTCCGGGGTCGTTCGGCTCGGCTGTGCCAGTTCGGCGTCGGGCTCCCGCTTCTGCTGGGG CTGCTGCCTCTCGCCCACCGGCCCGGCCGGCCCGAGCAGCGCGGAACCGAGCCGGATCCGGGTCCACCCG GCCCGGCCATGAGCGCTGCCGCATGATGCGTCGCCGCCCGCGCCGTTGCAGCCGCCGGCCACCAGGAGCC TCAGCACTGGGCGACCCCGCCCGGGCCTCGGGCCACATCACACAGGACAGCATGCGGCTTACAGTCCTGA AAAAAAGGCACAAGTGCTCCCATGATGCTTAAAAGCAACTTGTAAGGGTGTTTCAGGGAATCATGCAGCC GTCGGGACACAGGCTCCGGGACATCGAGCACCATCCTCTCCTGACTGACAATGACAATTACGACTCAGCA TCCTCTTCGTCCTCCGAGACTGACATGGCAGACAGGGTGTGGTTCATCCGAGATGGCTGTGGCATGGTCT GTGCTGTCATGACGTGGCTTCTCGTCGTCTATGCAGACTTCGTGGTGACCTTTGTCATGCTGCTGCCTTC CAAAGACTTCTGGTACTCCGTGGTGAACGGAGTCCTCTTCAACTGCTTGGCGGTGCTCGCGCTGTCCTCC CACCTGAGAACCATGCTCACTGACCCGGGTGCTGTCCCCAAAGGCAACGCCACGAAGGAGTACATGGAGA GCTTGCAGCTGAAGCCAGGCGAGGTGATCTACAAGTGCCCCAAGTGTTGCTGCATCAAGCCGGAACGTGC CCACCACTGCAGTATTTGCAAGAGATGCATTCGAAAGATGGACCATCACTGCCCGTGGGTGAACAACTGC GTCGGGGAGAAGAATCAGAGGTTCTTCGTGCTTTTCACCATGTACATAGCTCTGTCTTCGGTTCATGCTC TGATTCTCTGTGGGCTTCAGTTCATCTCCTGCGTCCGAGGGCAGTGGACAGAGTGCAGCGACTTCTCTCC TCCCATAACTGTAATCCTGTTGGTCTTCCTGTGCCTTGAGGGCCTCCTGTTCTTCACCTTCACCGCAGTC ATGTTCGGCACCCAGATCCACTCGATATGCAATGATGAAACAGAGATCGAGAGGCTGAAGAGCGAGAAGC CCACGTGGGAGCGCAGGCTGCGGTGGGAAGGAATGAAGTCTGTCTTCGGGGGCCCTCCCTCCCTCCTCTG GATGAACCCCTTCGTTGGCTTCCGACTCAGGCGGCTGCAGATGAGGACCAGGAAAGGAGGCCCCGAGTTC TCTGTCTGAGCCTTCAGGGTGGGAATGTCTGCAGACATCCGTGCTCAGTGGCCTGCGTGCACAGCAGCCT TGTGGGCAGATGGAGCGGGCCAGCTGACGGGGCCATGAGCGGCTTCATGGACCACAGGCCACAGGCACTT GTCATTTCTCTTGTGTACTGCCAGGAGAAGCATGCAGTCCCGGGAGGCCATCGTCTCACCATCCTGACCC TTGGGATGTCGCGTGGGCACCCACGGGTGGAGTTGCTTCTCAGTCATGAAGAAAGCCAGCACAGCAGCGC TGGGAGTCCTGCTGGCCCATCCCCAGCGCACCCCCTTACCCGAAAGAAGGGGTTCCACAGAGACTCTGCC TTGGAGGCCTCTGACACCCAGCACTGAACAGTGAAAGCAGCTTCCAGAACACTCCCTGCTGCCCCGGAAC GGGCATCCTTTGACCCTTCCAGCAAGTCTGTGTGTGCTCATCCTTGTGTGTCTGTGTGGTGTGTGTGTGT GTGTGTGTGTGAGCATGTGTCAGAGTCAGCAGTGTCGTTGAGGCACGTATGTGGTAGACAAGCTGTCTTT CAGACTCCAGTGTTTCAGGGAAGTGGGACGCACTGCTGGCCCTTGCTGGCCTACTATGAATGTATCCTAC AGTGTCCAGATCCAAAGAACAGGCATGCCTGTGCATGGGCCCGGCCTCCTGCTTTTATTTCACTGAAAAT TTAGTGTTTGAGGGCGTGGAGCTGAGGTTTTGTTGTTCCAGCCAAACTGCAACTGCAACAGTCTCTCAAC AGGGAGAGTGGTAGCCCATGCTGAGGCCCAGCGCTTTGGGGCAGGGGCCATGAGGAGCTCAGCGTGAGAA GCCACCTAGGGCCCCTTGGGGCAGGTGCTGCAGGGTGAGACAGCCTGGTGCCTGAGCTGTCAGAGTGGTG ATATGTGACTATTTCTCTACCCATGGCACCGCAGTCAGTGGAGCCTTGGTGCGGGCTTAGAATCCCGTCA GTACACAAACAGCTCCCCTCAGGGGATTTGCCTTAACCGCTGGTTGTTGCTTTGCTGCTGGGTGAGAGCA GTCTGACAGAGTCTGGGGACTTCCTGAGAGTCTTCTCCCATGCAGACACCAGTGTTTCCCTTCTGGGACC AGCTGCCTTTCTGTCCCTGGGACACCATGACTGTTCTTTCGGTGTCAGTTGCTTGTGCCCTGCCCTCATC TGGGCTCCTGTGCTACTGAGGTGACCCCCAGGGACCTGAGCCGAGCCCTCATGCAGCGAGCAATCTCTGG GGCCCAAGTCCCTGCAGAGGGACATCGAGGCCTCAGATTGTCTCTGCGTTCCTTTGCACACGTGTCTGGG CATGTAGGCTCTGGCGGTCTCACCCTCCAGTGTGGAGTCTGTGCGGTGAACCCAAGCTGAGTATACTTGA CTTTTCAATTCCTCAGTGTGATTTTATATGAAATAAAGTAACCCGTCCACAGTTAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:30605163 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30605163 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17711 same embryo
	EMAGE:17708 same embryo
	EMAGE:17710 same embryo
	EMAGE:17706 same embryo
	EMAGE:17707 same embryo
	EurExpress:euxassay_006041 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source