EMAGE mouse gene expression spatial database

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EMAGE:17711

Nkain3 Na+/K+ transporting ATPase interacting 3 ( MGI:2444830)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006033. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006033_01	euxassay_006033_02	euxassay_006033_03	euxassay_006033_04

euxassay_006033_05	euxassay_006033_06	euxassay_006033_07	euxassay_006033_08	euxassay_006033_09

euxassay_006033_10	euxassay_006033_11	euxassay_006033_12	euxassay_006033_13	euxassay_006033_14

euxassay_006033_15	euxassay_006033_16	euxassay_006033_17	euxassay_006033_18	euxassay_006033_19

euxassay_006033_20	euxassay_006033_21	euxassay_006033_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17711_wholemount_strong.wlz

17711_wholemount_moderate.wlz

17711_wholemount_weak.wlz

17711_wholemount_possible.wlz

17711_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17711_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9563
Entity Detected:	Nkain3, Na+/K+ transporting ATPase interacting 3 ( MGI:2444830)
Sequence:	sense strand is shown >T9563 CTGCATACTCCCCTCCCCTAAAAGACATTCACCCACTCACCAGCTGTAGCCTCTTCCATCCCCCGCCCTC CGCCCGGCTCCCCCTCCTCAAGAAGCCGACCCTCGCGCTAGGAGGTAGGGACCCCTGGCAGACGCACTGC ACGGGCGCGCCGCGCTGACCTCCGCCCGCCCTGCACGCAGATTAACAAAGGCGACAGCGAGCCCCAGTCC GAAAGCAGAAAGCCGGCCGCGGGGTCCGGAGCCTGCGCCCGGTCAGTCGGGGAGAGAGCCGCGCGGGCTG CTCTGAAGTCGCAGGATACAGCCGGAGCTGCCAGATCTCTCTCAAGCGGCGCTGCTGGGACGCTGGCCGC ACCATGGGCTGCTGCACCGGGCGCTGCTCGCTGGTCTGTCTCTGCGCACTGCAGCTGCTTTCAGCATTGG AGAGGCAGATCTTTGACTTCCTTGGCTTCCAGTGGGCACCCATTCTTGGAAATTTCCTGCATATAATAGT TGTCATATTGGGTCTGTTTGGAACCATTCAGTATAGACCACGTTACATCATGGTGTATACAGTGTGGACT GCTCTCTGGGTCACTTGGAATGTGTTCATCATCTGCTTTTATTTGGAAGTAGGAGGCCTCTCTAAGGACA CAGATCTGATGACCTTCAACATCTCTGTACACCGGTCATGGTGGAGGGAGCATGGACCTGGCTGTGTCCG GAGAGTGCTACCACCATCAGCCCATGGCATGATGGATGACTACACATACGTCTCAGTCACAGGCTGTGTT GTCGATTTCCAGTACCTAGAGGTCATCCACAGCGCTGTACAAATACTGCTATCGGTTAGAAGAACCAAGA CAGGTCTGCTATAGGAAGACAAAAGATTTGTATTTGTTAAGCATAGATCTCATGCAGTGGTGTAGAGTAT GGAATGTATAATGAGACCCAGACATGAGGGAAGTGAAATACAAAGCTATTTTAAAGGCCCCAAACAAGAG AAAAAAAAATGGCACAGGAGGTGGGAGGTGAGGGGTGGTGGGGATACAAGGAAAAATGCCTTGTCTGAGA AACAAGATCTGTAGGCTCCATTGAAATAGGTTGAAGATGAGCAACTAAGAGTTCTATGTCACCTTGGGAG ACTGTTCTGAGAGTTACATGGCCAGGTGATTTCATACTTATACTTCGGAAAAAGCCAAGCTACCTGTCAC TTCCTGCTGGCCTTAAACTACAAACCTGAATGTCTTATTATAAGCAATTGTGACATAATGATAATTGTTT GTGCATCTAAACATATCTGGCTATGAAAAAGATCCAGTACTAATTAGATGATATGAAATTGTTTTAGTTC TGTTTGTAATATTAGGGGACTACAATCTTCCTGTGGTTTATTCTTAACTAAAACACCATTATGAATAATA TAACTGTAGTTGATAGTAACATTGTGATCCATGATTGAGAATACAAAGTAGGATTAGGAGAAAACCGGCA TGAATGTGAGGTTTAAACGTCTTAAATTACCTACAATTACCAACTCAGAACTAAAGTATAATCTATTGCT TCTAGCCTGAGATTCCCGTGTGCCACAGAGTGATGAGAATATGACCAAACATCAAACTTTAAATTTAGTT AAAACCATATGAGGTATGTTTGCATCTTTTTTGTTGTTGTTGTTGTTTAATTGCACAGTTCTCAAGTGTC AACTTTCAAGTTTTCCTATAGTGATGTTGTGTCACAATGTCCAAAGCCTAGACATACTGGAAATAAGGAA AAAGACAAGACCACATGAGCCAATTCATTAAGGTATTAATTCAAAAGTCACTAGGTAAAACGCAATCAGC AGAGGCAAAAGAAGAGTCATCCAGAAGCCTAAGGAAACAGGGAGAACTCAGGGAGTCCTTGGTAATGAGA AACTGGTGTGTTGCATGACTCTAACATCCATGAAGACGAAGCCTGGATGAGCAAATACAAACACAAGTAT ACACAGTTAAATAATGAGAAGCAATCTGAGCTTACAAAGCTTTTATTTTTGCCTTGTTTCCTGCTTGTTT GTATTCTCCTGAACCTATAACGGGCATAATTATAAGCAAGAGTACTTCAAAGAATGCTCATTGTGTTAAA CTTTGAGATATTTACAAGATTCAAAGCCTAAATTATCTTTGTGGGAATGAGCTTTGAGCTTTTCATTTCT TTAAAATGAAAACAGAGTTCAGAATGATATTAAATATTTTCTTCTCTAACGTTTTTTCCTTCTTTATTGA TAGTATACAATTCTTTTTATCCTGTGCCTGGCAAATTCATTAACAAATATTTCACTGTCAAACAGATGAC AATCTGTAGCCCAGCTTAACCCTCGTCATTCATTAGAAGAGAGATGGGAAATGGGGAACTTCAATTTCTC TCTTACTGTATCAAGTCTGAAATCTTACCTCCACATCAAAGGTTAGCTTTAACACTCAAAAAAGAATGCC TTTCGTGTCATTAGGAAAGTGTCTGTCATGAAGATTCTCAACCTTGGAAAGAGAACAGGACATGGACTGT TCACCAGTGCTCCACATCAGTGAAATAGGTACATTCATAAGAAACAATAAGGACTTTTGCAACAAGAAAC AGAATTTTGGAAATAGCATCCTTAGTTCACTTTTAATTGTCAATTTCAGTGACTATAAATTCCTATATCA TATGCTAGCAGATACATATATGTGCCATGAGGTACATCTTTAGCTCAGGTAATCAAATATCAGTTGACAT TATAAAACTTGCTAAGAACTTTATATGCCCCAGTACAGGGGAACACCAGGGCCAAAAAGGGGGAGTGGGT GGGCAGGGGAGTGGGGGTGGGTGGATATGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAA TACCTAATAAAAAATGGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30613986 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30613986 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17708 same embryo
	EMAGE:17710 same embryo
	EMAGE:17706 same embryo
	EMAGE:17709 same embryo
	EMAGE:17707 same embryo
	EurExpress:euxassay_006033 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source