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EMAGE:17775

Trim66 tripartite motif-containing 66 ( MGI:2152406)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775
"Pseudo-wholemount" of euxassay_008949. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008949_01 euxassay_008949_02 euxassay_008949_03 euxassay_008949_04
EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775
euxassay_008949_05 euxassay_008949_06 euxassay_008949_07 euxassay_008949_08 euxassay_008949_09
EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775
euxassay_008949_10 euxassay_008949_11 euxassay_008949_12 euxassay_008949_13 euxassay_008949_14
EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775 EMAGE:17775
euxassay_008949_15 euxassay_008949_16 euxassay_008949_17 euxassay_008949_18 euxassay_008949_19
EMAGE:17775 EMAGE:17775 EMAGE:17775
euxassay_008949_20 euxassay_008949_21 euxassay_008949_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17775Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17775_wholemount_strong.wlz
17775_wholemount_moderate.wlz
17775_wholemount_weak.wlz
17775_wholemount_possible.wlz
17775_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17775_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35476
Entity Detected:Trim66, tripartite motif-containing 66 ( MGI:2152406)
Sequence:sense strand is shown

>T35476
GTCCCCTTTCCTGAGTAGGACTTATGTGGTCACTGTGAAATGCTGTGTGTGGTTTGGTACTCTTGGCTGA
GCTGGGTGGCAGTGCAAAGATCAGTTTCCACTCACACCCAGGAGATGCCAGCTTCAGAGTCTGTCCCTCT
GTGTCCTAACTCTGGGATAATAACACTCCAGAGCCTGCCTTCCCAGAAGTCACTGGTATGTGTGCCAGTG
GCTCTTCCTTGATTTCAGACATCTGCATCTTGAGTTCCACTTGGGACTAATGTGACATGATGTCTGCTGC
AGGAACTGAGGTCCCTGGCAAGGATGACCCACAGCCAGTGCAGAGTGTCAGGCCTCTCCTGCCTACGATT
CTAGATCTCTACTTTTTTCACTCAGCCAAAGATCAGAACTGTGGCCATGATGCTCACAGCCCGGAAGCAG
GCAACACTGTCACCCTTTAGTGTTGGAACTTGGAGAAGGCTACAGTGACTGGTAGGAGTCTGCTGAGGTG
TTAGTAGGCTAAGGGTGGAGAAGGGGAAAATTGGGAGGTGAGAAGCCTTAGGTGACTCTGAGGTTACATC
AGGGTTGTAGTCCTCACAGAATGCAGAAAGGCCCTTCATGGGAATGGGGTGGTGGCAACAGAGCTACAGG
CCACATAGTGGAAGGATCACTACAGTATAGGCAGGGCGTTTGTGAGGACGCCTCTGAGACACATACAGGG
ACAGGCTTCTTCGAAAGCAAGGGCTTGGCTGAGGAAGGACCTGTCGACTGTACTGCTGATCGAGCGACAG
GCCTGGCATTTCAGTGGCCTGGTCCTGAGGCATGGCATGGCTGATTTCAGGGGACGCTTGGGTCGTGGCT
TTGTTTTTGTGAAGAGGTGCAGTTTAAGGAAGTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101244. Forward Primer - name:101244_F_cDNA_Kiaa0298-hp, sequence:GTCCCCTTTCCTGAGTAGGACT; Reverse Primer - name:101244_N_SP6_cDNA_Kiaa0298-hp, sequence:GCACTTCCTTAAACTGCACCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17774 same embryo
 EMAGE:17776 same embryo
 EMAGE:17773 same embryo
 EMAGE:17772 same embryo
 EurExpress:euxassay_008949 same experiment
 MGI:4828919 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS