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EMAGE:17916

Stx11 syntaxin 11 ( MGI:1921982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916
"Pseudo-wholemount" of euxassay_011673. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011673_01 euxassay_011673_02 euxassay_011673_03 euxassay_011673_04
EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916
euxassay_011673_05 euxassay_011673_06 euxassay_011673_07 euxassay_011673_08 euxassay_011673_09
EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916
euxassay_011673_10 euxassay_011673_11 euxassay_011673_12 euxassay_011673_13 euxassay_011673_14
EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916
euxassay_011673_15 euxassay_011673_16 euxassay_011673_17 euxassay_011673_18 euxassay_011673_19
EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916 EMAGE:17916
euxassay_011673_20 euxassay_011673_21 euxassay_011673_22 euxassay_011673_23 euxassay_011673_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17916Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17916_wholemount_strong.wlz
17916_wholemount_moderate.wlz
17916_wholemount_weak.wlz
17916_wholemount_possible.wlz
17916_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17916_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
humerus
moderate moderate
regionalmoderate expression: see section 02 weak expression: see section 01 03
fibula
moderate moderate
regionalmoderate expression: see section 02
tibia
moderate moderate
regionalmoderate expression: see section 01 02
femur
moderate moderate
regionalmoderate expression: see section 03 06 weak expression: see section 02 04 05
scapula
moderate moderate
regionalmoderate expression: see section 02 weak expression: see section 03
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37095
Entity Detected:Stx11, syntaxin 11 ( MGI:1921982)
Sequence:sense strand is shown

>T37095
AAGCCATGTACGAGTACAACCAGGCCGAGATGAAACAGCGCGACAACTGCAAGATCCGCATCCAGCGGCA
GCTGGAGATCATGGGCAAGGACATGTCGGGCGAGCAGATTGAGGACATGTTCGAGCAGGGCAAGTGGGAT
GTATTCTCCGAGAACCTGCTGGCCGACTTGAAGGGCGCGCGGGCTGCGCTCAACGAGATCGAGAGCCGCC
ACCGCGAGCTCCTGCGCCTGGAGGGTCGCATTCGCGATGTGCACGAGCTCTTCCTACAGATGGCCGTGCT
AGTGGAGAAGCAGGAGGACACGCTGAACGTCATCGAGCTTAACGTGCAGAAGACCCTCGATTACACGGGC
GAGGCCAAGGCGCAGGTGCGCAAGGCGGTGCAGTACAAGAAGAAGAACCCCTGCAGGACCATCTGCTGCT
TCTGTTGTCCCTGTGTCAACTAGCCGCTGGCTTGAGTCTCCACTGCACGTGCCATGTGGGAAACATGTCC
AGGATGCCCAGCCAAAGGCATCTTGCACTGGGCCGAGGGAGAGGGTCCCAATACCTTGCAGAATTCAGAG
CATAGAGAAAGTGGGGGTTGGGATGCAAGATTTGGAGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82451. Forward Primer - name:082451_F_cDNA_Stx11, sequence:AAGCCATGTACGAGTACAACCA; Reverse Primer - name:082451_N_SP6_cDNA_Stx11, sequence:GACTCCAAATCTTGCATCCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17914 same embryo
 EMAGE:17917 same embryo
 EMAGE:17915 same embryo
 EMAGE:17913 same embryo
 EurExpress:euxassay_011673 same experiment
 MGI:4828521 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS