EMAGE mouse gene expression spatial database

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EMAGE:18641

Cdh12 cadherin 12 ( MGI:109503)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016537_01	euxassay_016537_02	euxassay_016537_03	euxassay_016537_04

euxassay_016537_05	euxassay_016537_06	euxassay_016537_07	euxassay_016537_08	euxassay_016537_09

euxassay_016537_10	euxassay_016537_11	euxassay_016537_12	euxassay_016537_13	euxassay_016537_14

euxassay_016537_15	euxassay_016537_16	euxassay_016537_17	euxassay_016537_18	euxassay_016537_19

euxassay_016537_20	euxassay_016537_21	euxassay_016537_22	euxassay_016537_23	euxassay_016537_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18641_wholemount_strong.wlz

18641_wholemount_moderate.wlz

18641_wholemount_weak.wlz

18641_wholemount_possible.wlz

18641_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18641_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 02 03 weak expression: see section 22

femur

moderate

regional

moderate expression: see section 02 03

hindgut

weak

regional

weak expression: see section 12

midgut

weak

regional

weak expression: see section 10 11 12 13 17 18

bladder

weak

regional

weak expression: see section 10 11 13

scapula

moderate

regional

moderate expression: see section 02 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38965
Entity Detected:	Cdh12, cadherin 12 ( MGI:109503)
Sequence:	sense strand is shown >T38965 AGACCTTGATGTGGGCAGTAGTGCTGTTAGGTACTTCATAGATTGGAAGAGTGATGGGGAGAGCTACTTT ACAATAGATGGAACGGAAGGAACCATCGCCACTAATGAATTACTAGACAGAGAGAACATGGCGCAGTATA ATTTCTCCATAATTGCAAGTAAAGTTAGTAACCCATTATTAACTAGCAAAGTTAACATACTAATTAATGT CCTAGATGTCAATGAATTTCCTCCAGAAATATCTGTGCCATATGAGACAGCTGTATGTGAGAATGCCAAG CCAGGACAGATAATTCAGATAGTTGGTGCTGTAGACCGAGATCTCTCACCTGCTGGGCAGCAATTCTCCT TTAGATTAGCACCAGAGGCTGTCATCAAACCAAATTTTACAGTCCATGACTTCAGAAACAACACAGCTGG AATCGAAACCCGAAGAAATGGCTATAGCCGCAGGCAGCAGGAGTTGTATTTCCTTCCCATTGTAATAGAA GACAGCAGTTATCCTGTCCAGAGCAGCACGAATACAATGACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 265627. Forward Primer - name:265627_F_cDNA_Cdh12, sequence:AGACCTTGATGTGGGCAGTAGT; Reverse Primer - name:265627_N_SP6_cDNA_Cdh12, sequence:AGTCATTGTATTCGTGCTGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18646 same embryo
	EMAGE:18643 same embryo
	EMAGE:18645 same embryo
	EMAGE:18644 same embryo
	EMAGE:18642 same embryo
	EurExpress:euxassay_016537 same experiment
	MGI:4823763 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source