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EMAGE:18642

Gpr17 G protein-coupled receptor 17 ( MGI:3584514)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642
"Pseudo-wholemount" of euxassay_016541. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016541_01 euxassay_016541_02 euxassay_016541_03 euxassay_016541_04
EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642
euxassay_016541_05 euxassay_016541_06 euxassay_016541_07 euxassay_016541_08 euxassay_016541_09
EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642
euxassay_016541_10 euxassay_016541_11 euxassay_016541_12 euxassay_016541_13 euxassay_016541_14
EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642
euxassay_016541_15 euxassay_016541_16 euxassay_016541_17 euxassay_016541_18 euxassay_016541_19
EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642 EMAGE:18642
euxassay_016541_20 euxassay_016541_21 euxassay_016541_22 euxassay_016541_23 euxassay_016541_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18642Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18642_wholemount_strong.wlz
18642_wholemount_moderate.wlz
18642_wholemount_weak.wlz
18642_wholemount_possible.wlz
18642_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18642_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
strong strong
single cellstrong expression: see section 10 12 moderate expression: see section 09 weak expression: see section 07 08
medulla oblongata basal plate ventricular layer
strong strong
single cellstrong expression: see section 11
ventral grey horn
strong strong
single cellstrong expression: see section 10 11 moderate expression: see section 09 weak expression: see section 08
stomach
weak weak
regionalweak expression: see section 01 02 03 04 05
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38975
Entity Detected:Gpr17, G protein-coupled receptor 17 ( MGI:3584514)
Sequence:sense strand is shown

>T38975
GACCAAGTAGGGCAACATCTTCCTGGTTGTGGTTCTGGTGGCCTTGTGACATGTCTGTCCAGGCATGTGC
GTTAAGGGAGCTCTCAAACGGATCCCTCCAGCTGCATCCTGCCTTTCACCAGAGAAACCCTAAGATGGCC
CTGATGCTCAGCACTGCTTCTATCTGGTGCTGATCTGTCCACCCCCATGGCCACAACAACCTGCGGAGTA
CAAGACTGTGCCAGCCAGGACCAGCACGGGACCATGTGTCTCTTCGACAGAAGAGCAAAGGGACAAGAGT
ACCGCAGTCTGTGACAGGAGGGACAGAGGGGGCAAAGACCCAGACAGGAAGGACAGTCTCCTGGGAAACT
GGAGGGTCTCTGGAAATTAGCACTGCCTTTCCTGACACAGGGGTTCAGAAGCAGACTTGGGTCAGAGGAG
GAGAAATTTGCAGCTCGATTTGGGACCTGTTACATTAGAATCTTAAACAGAGATGCCACACTGGTAGGAG
ACTTGAGCAAAGGAGAGATCTGGAGGGGATACCTGGATTGTCCAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 265952. Forward Primer - name:265952_F_cDNA_Gpr17, sequence:GACCAAGTAGGGCAACATCTTC; Reverse Primer - name:265952_N_SP6_cDNA_Gpr17, sequence:TCTGGACAATCCAGGTATCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18646 same embryo
 EMAGE:18641 same embryo
 EMAGE:18643 same embryo
 EMAGE:18645 same embryo
 EMAGE:18644 same embryo
 EurExpress:euxassay_016541 same experiment
 MGI:4825180 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS