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EMAGE:18846

1700036A12Rik RIKEN cDNA 1700036A12 gene ( MGI:1920548)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846
"Pseudo-wholemount" of euxassay_014066. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014066_01 euxassay_014066_02 euxassay_014066_03 euxassay_014066_04
EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846
euxassay_014066_05 euxassay_014066_06 euxassay_014066_07 euxassay_014066_08 euxassay_014066_09
EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846
euxassay_014066_10 euxassay_014066_11 euxassay_014066_12 euxassay_014066_13 euxassay_014066_14
EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846
euxassay_014066_15 euxassay_014066_16 euxassay_014066_17 euxassay_014066_18 euxassay_014066_19
EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846 EMAGE:18846
euxassay_014066_20 euxassay_014066_21 euxassay_014066_22 euxassay_014066_23 euxassay_014066_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18846Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18846_wholemount_strong.wlz
18846_wholemount_moderate.wlz
18846_wholemount_weak.wlz
18846_wholemount_possible.wlz
18846_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18846_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40276
Entity Detected:1700036A12Rik, RIKEN cDNA 1700036A12 gene ( MGI:1920548)
Sequence:sense strand is shown

>T40276
ACCCTCCTACTCTTCCATCCTCAGCCGTTTGTGTGGGCTTCCACGTTCTGTTGGCCGCCCCCTGGAAAAT
GCCTCGCTTGTGCTGTTATTTTTTCTTTTATGTTGAAGTCTCCTGGGGTCCGCTTGACTCTTGTAGTTGG
TTAGAGTCCAAGTATAGACTCCTCTGCTTGTGACTAGAGGCAGGGCAGTAGGGTAAGGCTGGTGGGAATC
AGGGAGAGGGGGCTTTTCCACATTTTAGAAGATAATATAATAACATGGTTGAGGTTGAGATAGAAATACT
TCAGAGGAAGCGTGACTATGAGACAGAGGGAAGAAGACAGATCCCACCATCCCACCAACTGATGGCTGGT
GGGAACTCTGCTCCCACCACCCGCACTGCTCACCCTAGCCCCAACACCAGACACCCTTCCAGACTGAGTA
AGGCTAAGCCGCGGACTGGATCAAGGATTGGAACCTGCCTACCAGAAGAAAGTCAGAAAAGTGTCTATAA
GGGCAAGATTGGGTTTCCTTTAACATGAGAGGGATATGGTGTGGGGCAGGGAATGCCACCCTGTCTCTTC
CTAGGAGGTGCTCCTGTGAGCCTTCCCCCGGGCCCTGTCAATCATGCAACCTTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81973. Forward Primer - name:081973_F_cDNA_1700036A12Rik, sequence:ACCCTCCTACTCTTCCATCCTC; Reverse Primer - name:081973_N_SP6_cDNA_1700036A12Rik, sequence:TAAGGTTGCATGATTGACAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18849 same embryo
 EMAGE:18848 same embryo
 EMAGE:18850 same embryo
 EMAGE:18847 same embryo
 EurExpress:euxassay_014066 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS