EMAGE mouse gene expression spatial database

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EMAGE:18943

LOC671981 (LOC671981)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015958. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015958_01	euxassay_015958_02	euxassay_015958_03	euxassay_015958_04

euxassay_015958_05	euxassay_015958_06	euxassay_015958_07	euxassay_015958_08	euxassay_015958_09

euxassay_015958_10	euxassay_015958_11	euxassay_015958_12	euxassay_015958_13	euxassay_015958_14

euxassay_015958_15	euxassay_015958_16	euxassay_015958_17	euxassay_015958_18	euxassay_015958_19

euxassay_015958_20	euxassay_015958_21	euxassay_015958_22	euxassay_015958_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18943_wholemount_strong.wlz

18943_wholemount_moderate.wlz

18943_wholemount_weak.wlz

18943_wholemount_possible.wlz

18943_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18943_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 05 06 14 15

facial vii ganglion

weak

regional

weak expression: see section 02 03 04 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 15 16

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 21

trigeminal v nerve

weak

regional

weak expression: see section 15

dorsal root ganglion

weak

regional

weak expression: see section 05 07 08 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40520
Entity Detected:	LOC671981, (LOC671981)
Sequence:	sense strand is shown >T40520 GGTCCTGCTTTCTGAAATCCTACACCTTCTATGCAGACAAATGAAGTTGGACTTCACGGAATCCGAAGGG CCCTGCTCCTCTGAGGCACTGTCAAAGAAGGAGCTGTCTGCCGAGGAGCTGTCTCAGCGCCTGGAAAAGC TCATCATGGAGGAGAAAGCGGATGACGAGCGGATCTTTGACTGGGTGGAGGCTAATCTCGACGAAAGCCA GATGAGTTCGCCTACATTCCTTAGAGCTTTAATGACAGCCGTTTGCAAAGCAGCTATCATAGCTGACTGT TCTACCTTCAGAGTGGACACTGCTGTGATCAAGCAGAGAGTGCCGATCTTACTCAAGTACCTAGACTCAG ACACGGAGAAGGAACTACAAGCACTTTATGCACTACAAGCATCCATAGTAAAACTTGACCAACCTGCCAA CTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 164157. Forward Primer - name:164157_F_cDNA_mCG14542.1, sequence:GGTCCTGCTTTCTGAAATCCTA; Reverse Primer - name:164157_N_SP6_cDNA_mCG14542.1, sequence:CAAGTTGGCAGGTTGGTCAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18944 same embryo
	EMAGE:18942 same embryo
	EMAGE:18945 same embryo
	EurExpress:euxassay_015958 same experiment
	MGI:4824516 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	BrainStars same gene
	ViBrism same gene
Data Source